(Circular) (Six-base) MAPSORT of: pFN21AB1290.seq Check: 3581 from: 1 to: 6952 pFN21AB1290 6952 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3262 3262 Size: 6952 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4295 1937 Size: 2358 4594 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3585 5015 6399 6667 1632 Size: 1953 1430 1384 268 1917 Fragments arranged by size: 1953 1917 1430 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 2188 3556 3979 5851 6828 1203 Size: 985 1368 423 1872 977 1327 Fragments arranged by size: 1872 1368 1327 985 977 423 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 2542 5681 2542 Size: 3139 3813 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2776 1184 Size: 1592 5360 AlwNI CAG_nnn'CTG Cuts at: 2209 4695 6267 2209 Size: 2486 1572 2894 Fragments arranged by size: 2894 2486 1572 ApaI G_GGCC'C Cuts at: 4203 4203 Size: 6952 ApaLI G'TGCA_C Cuts at: 5009 6165 5009 Size: 1156 5796 ApoI r'AATT_y Cuts at: 1493 2626 2803 4075 4152 4261 4487 4639 6748 Size: 1133 177 1272 77 109 226 152 2109 Cuts at: 6748 1493 Size: 1697 Fragments arranged by size: 2109 1697 1272 1133 226 177 152 109 77 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2214 3474 3621 4277 1870 Size: 73 271 1260 147 656 4545 Fragments arranged by size: 4545 1260 656 271 147 73 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2930 3419 4273 5728 618 Size: 325 217 627 1143 489 854 1455 1842 Fragments arranged by size: 1842 1455 1143 854 627 489 325 217 BanII G_rGCy'C Cuts at: 1391 2513 2644 2849 3192 3901 4148 4203 4271 Size: 1122 131 205 343 709 247 55 68 Cuts at: 4271 1391 Size: 4072 Fragments arranged by size: 4072 1122 709 343 247 205 131 68 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5981 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5143 5177 1903 Size: 34 3206 34 3678 Fragments arranged by size: 3678 3206 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4844 6054 4844 Size: 1210 5742 BclI T'GATC_A Cuts at: 1548 3868 1548 Size: 2320 4632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 3617 5563 Size: 107 122 71 631 605 368 1577 1946 Cuts at: 5563 136 Size: 1525 Fragments arranged by size: 1946 1577 1525 631 605 368 122 107 71 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 2657 4369 2657 Size: 1712 5240 Bme1580I G_kGCm'C Cuts at: 1790 4203 5013 6169 1790 Size: 2413 810 1156 2573 Fragments arranged by size: 2573 2413 1156 810 BmgBI CAC'GTC Cuts at: 1318 2225 1318 Size: 907 6045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4683 5641 442 Size: 416 53 83 399 24 3266 958 1753 Fragments arranged by size: 3266 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2249 1056 Size: 1193 5759 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2516 3594 2516 Size: 1078 5874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2616 2766 3267 3285 3303 3321 3815 Size: 856 521 150 501 18 18 18 494 Cuts at: 3815 3927 5612 1239 Size: 112 1685 2579 Fragments arranged by size: 2579 1685 856 521 501 494 150 112 18 18 18 Bpu10I CC'TnA_GC Cuts at: 3081 3081 Size: 6952 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 3833 5077 5942 6240 2420 Size: 1413 1244 865 298 3132 Fragments arranged by size: 3132 1413 1244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3370 5615 882 Size: 617 1871 2245 2219 Fragments arranged by size: 2245 2219 1871 617 BsaAI yAC'GTr Cuts at: 493 1204 2189 4743 493 Size: 711 985 2554 2702 Fragments arranged by size: 2702 2554 985 711 BsaBI GATnn'nnATC Cuts at: 6946 6946 Size: 6952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2696 4759 5141 5776 Size: 53 83 186 936 1163 2063 382 635 Cuts at: 5776 275 Size: 1451 Fragments arranged by size: 2063 1451 1163 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5385 5783 6057 6204 6615 5385 Size: 398 274 147 411 5722 Fragments arranged by size: 5722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3931 3961 1178 Size: 30 165 30 2528 30 4169 Fragments arranged by size: 4169 2528 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2369 2377 2393 2417 2593 3449 3520 Size: 1165 18 8 16 24 176 856 71 Cuts at: 3520 3602 3605 3647 3671 3695 3830 1186 Size: 82 3 42 24 24 135 4308 Fragments arranged by size: 4308 1165 856 176 135 82 71 42 24 24 24 18 16 8 3 BseYI C'CCAG_C Cuts at: 3295 3313 3331 3355 6155 6651 3295 Size: 18 18 24 2800 496 3596 Fragments arranged by size: 3596 2800 496 24 18 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2547 2775 3909 1163 Size: 573 811 228 1134 4206 Fragments arranged by size: 4206 1134 811 573 228 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2644 3211 3901 4022 5013 5098 6169 2644 Size: 567 690 121 991 85 1071 3427 Fragments arranged by size: 3427 1071 991 690 567 121 85 BsmI GAATG_Cn' Cuts at: 3394 4463 4556 3394 Size: 1069 93 5790 Fragments arranged by size: 5790 1069 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 2644 2849 3192 3211 3901 4022 Size: 399 723 131 205 343 19 690 121 Cuts at: 4022 4148 4203 4271 5013 5098 6169 1391 Size: 126 55 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 690 399 343 205 131 126 121 85 68 55 19 BspEI T'CCGG_A Cuts at: 6615 6615 Size: 6952 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3262 4307 844 Size: 2418 1045 3489 Fragments arranged by size: 3489 2418 1045 BsrBI CCG'CTC Cuts at: 1394 1823 2320 2359 3247 3661 3676 4839 1394 Size: 429 497 39 888 414 15 1163 3507 Fragments arranged by size: 3507 1163 888 497 429 414 39 15 BsrDI GCAATG_nn' Cuts at: 65 1248 5447 5621 65 Size: 1183 4199 174 1396 Fragments arranged by size: 4199 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 5596 5783 1700 Size: 110 389 3397 187 2869 Fragments arranged by size: 3397 2869 389 187 110 BsrGI T'GTAC_A Cuts at: 96 3935 96 Size: 3839 3113 BssHII G'CGCG_C Cuts at: 1925 3691 1925 Size: 1766 5186 BssSI C'ACGA_G Cuts at: 5012 6024 5012 Size: 1012 5940 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6952 BstBI TT'CG_AA Cuts at: 2567 5756 2567 Size: 3189 3763 BstEII G'GTnAC_C Cuts at: 1184 3985 1184 Size: 2801 4151 BstYI r'GATC_y Cuts at: 1972 2530 3154 4106 4282 4649 5034 5051 6503 Size: 558 624 952 176 367 385 17 1452 Cuts at: 6503 6589 6601 6947 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 952 624 558 385 367 346 176 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2841 4209 2841 Size: 1368 5584 BtgI C'CryG_G Cuts at: 513 1065 2004 2503 2663 3288 3360 513 Size: 552 939 499 160 625 72 4105 Fragments arranged by size: 4105 939 625 552 499 160 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 2061 4468 5262 5282 2061 Size: 2407 794 20 3731 Fragments arranged by size: 3731 2407 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 4256 4256 Size: 6952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5959 809 Size: 507 4643 1802 Fragments arranged by size: 4643 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2715 4399 Size: 54 1425 50 275 18 202 683 1684 Cuts at: 4399 5288 6917 8 Size: 889 1629 43 Fragments arranged by size: 1684 1629 1425 889 683 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2310 2321 2611 2854 2921 3015 3237 Size: 921 36 11 290 243 67 94 222 Cuts at: 3237 3455 3513 3561 3774 4882 1353 Size: 218 58 48 213 1108 3423 Fragments arranged by size: 3423 1108 921 290 243 222 218 213 94 67 58 48 36 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2166 3101 3668 5539 5913 Size: 1064 138 435 142 935 567 1871 374 Cuts at: 5913 6059 387 Size: 146 1280 Fragments arranged by size: 1871 1280 1064 935 567 435 374 146 142 138 EcoICRI GAG'CTC Cuts at: 2642 3899 2642 Size: 1257 5695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2616 2766 3267 3285 3303 3321 Size: 393 463 521 150 501 18 18 18 Cuts at: 3321 3585 3815 3927 5015 5612 6399 6667 1239 Size: 264 230 112 1088 597 787 268 1524 Fragments arranged by size: 1524 1088 787 597 521 501 463 393 268 264 230 150 112 18 18 18 EcoNI CCTnn'n_nnAGG Cuts at: 4208 4208 Size: 6952 EcoO109I rG'GnC_Cy Cuts at: 4200 4200 Size: 6952 EcoRI G'AATT_C Cuts at: 4261 4639 4261 Size: 378 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4211 4211 Size: 6952 FspI TGC'GCA Cuts at: 5458 5458 Size: 6952 HaeII r_GCGC'y Cuts at: 1678 3668 3793 6099 1678 Size: 1990 125 2306 2531 Fragments arranged by size: 2531 2306 1990 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 2808 2840 3578 Size: 32 135 32 956 32 248 32 738 Cuts at: 3578 3610 4113 4145 5593 5625 5667 5699 1373 Size: 32 503 32 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 956 738 503 248 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2683 4296 4542 669 Size: 988 281 745 1613 246 3079 Fragments arranged by size: 3079 1613 988 745 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 2683 4542 2683 Size: 1859 5093 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 6066 6250 1305 Size: 1008 3753 184 2007 Fragments arranged by size: 3753 2007 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2717 10 Size: 54 1425 343 885 4245 Fragments arranged by size: 4245 1425 885 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2774 3931 4910 5269 5428 518 Size: 648 1608 1157 979 359 159 2042 Fragments arranged by size: 2042 1608 1157 979 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2735 3640 3646 4368 5045 6193 6438 Size: 600 529 905 6 722 677 1148 245 Cuts at: 6438 1606 Size: 2120 Fragments arranged by size: 2120 1148 905 722 677 600 529 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 2503 2663 3288 3360 513 Size: 552 939 499 160 625 72 4105 Fragments arranged by size: 4105 939 625 552 499 160 72 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NheI G'CTAG_C Cuts at: 1052 2245 1052 Size: 1193 5759 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 3983 4310 5855 1132 Size: 97 2754 327 1545 2229 Fragments arranged by size: 2754 2229 1545 327 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6952 PciI A'CATG_T Cuts at: 3979 5851 3979 Size: 1872 5080 PflMI CCAn_nnn'nTGG Cuts at: 1108 3269 1108 Size: 2161 4791 PfoI T'CCnGG_A Cuts at: 3949 3949 Size: 6952 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5021 5053 6534 6566 5021 Size: 32 1481 32 5407 Fragments arranged by size: 5407 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4091 4522 4091 Size: 431 6521 PspOMI G'GGCC_C Cuts at: 4199 4199 Size: 6952 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3832 3864 4206 4238 3832 Size: 32 342 32 6546 Fragments arranged by size: 6546 342 32 32 PstI C_TGCA'G Cuts at: 830 2655 3418 3645 4243 4304 830 Size: 1825 763 227 598 61 3478 Fragments arranged by size: 3478 1825 763 598 227 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 2735 3640 2735 Size: 905 6047 SacI G_AGCT'C Cuts at: 2644 3901 2644 Size: 1257 5695 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SapI GCTCTTCn'nnn_ Cuts at: 2274 2310 2854 3237 3513 3774 2274 Size: 36 544 383 276 261 5452 Fragments arranged by size: 5452 544 383 276 261 36 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SfcI C'TryA_G Cuts at: 826 1045 2651 3414 3641 3849 4239 4300 5435 Size: 219 1606 763 227 208 390 61 1135 Cuts at: 5435 6116 6307 826 Size: 681 191 1471 Fragments arranged by size: 1606 1471 1135 763 681 390 227 219 208 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 2216 3623 4279 1872 Size: 344 1407 656 4545 Fragments arranged by size: 4545 1407 656 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3812 5056 5957 6219 820 Size: 197 926 456 1413 1244 901 262 1553 Fragments arranged by size: 1553 1413 1244 926 901 456 262 197 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4310 1132 Size: 3178 3774 SspI AAT'ATT Cuts at: 5 52 4876 5 Size: 47 4824 2081 Fragments arranged by size: 4824 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6952 StyI C'CwwG_G Cuts at: 513 1065 2004 2503 2663 3288 3360 3891 4391 Size: 552 939 499 160 625 72 531 500 Cuts at: 4391 513 Size: 3074 Fragments arranged by size: 3074 939 625 552 531 500 499 160 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3046 3052 4979 4996 5149 5334 5779 Size: 1255 1466 6 1927 17 153 185 445 Cuts at: 5779 325 Size: 1498 Fragments arranged by size: 1927 1498 1466 1255 445 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2911 3935 5198 Size: 274 80 33 51 1443 934 1024 1263 Cuts at: 5198 6910 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1263 1024 934 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2694 2726 3903 3935 6912 6944 2694 Size: 32 1177 32 2977 32 2702 Fragments arranged by size: 2977 2702 1177 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6952 XbaI T'CTAG_A Cuts at: 2798 4288 2798 Size: 1490 5462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3358 3358 Size: 6952 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 2214 3621 4277 1870 Size: 344 1407 656 4545 Fragments arranged by size: 4545 1407 656 344 XmnI GAAnn'nnTTC Cuts at: 3767 5081 3767 Size: 1314 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI