Restriction map for
pFN21AB1290
(Linear) (Six-base) MAPSORT of: KIBB1290.seq Check: 7873 from: 1 to: 2247
KIBB1290.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1257 2247
Size: 1257 990
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1580 2247
Size: 1580 667
AflIII A'CryG_T
Cuts at: 0 183 1551 1974 2247
Size: 183 1368 423 273
Fragments arranged by size:
1368 423 273 183
AhdI GACnn_n'nnGTC
Cuts at: 0 537 2247
Size: 537 1710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 771 2247
Size: 771 1476
AlwNI CAG_nnn'CTG
Cuts at: 0 204 2247
Size: 204 2043
ApaI G_GGCC'C
Cuts at: 0 2198 2247
Size: 2198 49
ApoI r'AATT_y
Cuts at: 0 621 798 2070 2147 2247
Size: 621 177 1272 77 100
Fragments arranged by size:
1272 621 177 100 77
AvaI C'yCGr_G
Cuts at: 0 209 1469 1616 2247
Size: 209 1260 147 631
Fragments arranged by size:
1260 631 209 147
BanI G'GyrC_C
Cuts at: 0 925 1414 2247
Size: 925 489 833
Fragments arranged by size:
925 833 489
BanII G_rGCy'C
Cuts at: 0 508 639 844 1187 1896 2143 2198 2247
Size: 508 131 205 343 709 247 55 49
Fragments arranged by size:
709 508 343 247 205 131 55 49
BclI T'GATC_A
Cuts at: 0 1863 2247
Size: 1863 384
BglI GCCn_nnn'nGGC
Cuts at: 0 35 1612 2247
Size: 35 1577 635
Fragments arranged by size:
1577 635 35
BlpI GC'TnA_GC
Cuts at: 0 652 2247
Size: 652 1595
Bme1580I G_kGCm'C
Cuts at: 0 2198 2247
Size: 2198 49
BmgBI CAC'GTC
Cuts at: 0 220 2247
Size: 220 2027
BmtI G_CTAG'C
Cuts at: 0 244 2247
Size: 244 2003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 511 1589 2247
Size: 511 1078 658
Fragments arranged by size:
1078 658 511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 611 761 1262 1280 1298 1316 1810
Size: 90 521 150 501 18 18 18 494
Cuts at: 1810 1922 2247
Size: 112 325
Fragments arranged by size:
521 501 494 325 150 112 90 18
18 18
Bpu10I CC'TnA_GC
Cuts at: 0 1076 2247
Size: 1076 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1828 2247
Size: 415 1413 419
Fragments arranged by size:
1413 419 415
BsaI GGTCTCn'nnnn_
Cuts at: 0 1365 2247
Size: 1365 882
BsaAI yAC'GTr
Cuts at: 0 184 2247
Size: 184 2063
BsaHI Gr'CG_yC
Cuts at: 0 691 2247
Size: 691 1556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1926 1956 2247
Size: 1926 30 291
Fragments arranged by size:
1926 291 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 364 372 388 412 588 1444 1515
Size: 346 18 8 16 24 176 856 71
Cuts at: 1515 1597 1600 1642 1666 1690 1825 2247
Size: 82 3 42 24 24 135 422
Fragments arranged by size:
856 422 346 176 135 82 71 42
24 24 24 18 16 8 3
BseYI C'CCAG_C
Cuts at: 0 1290 1308 1326 1350 2247
Size: 1290 18 18 24 897
Fragments arranged by size:
1290 897 24 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 770 1904 2247
Size: 542 228 1134 343
Fragments arranged by size:
1134 542 343 228
BsiHKAI G_wGCw'C
Cuts at: 0 639 1206 1896 2017 2247
Size: 639 567 690 121 230
Fragments arranged by size:
690 639 567 230 121
BsmI GAATG_Cn'
Cuts at: 0 1389 2247
Size: 1389 858
Bsp1286I G_dGCh'C
Cuts at: 0 508 639 844 1187 1206 1896 2017 2143
Size: 508 131 205 343 19 690 121 126
Cuts at: 2143 2198 2247
Size: 55 49
Fragments arranged by size:
690 508 343 205 131 126 121 55
49 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1257 2247
Size: 1257 990
BsrBI CCG'CTC
Cuts at: 0 315 354 1242 1656 1671 2247
Size: 315 39 888 414 15 576
Fragments arranged by size:
888 576 414 315 39 15
BsrFI r'CCGG_y
Cuts at: 0 194 2247
Size: 194 2053
BsrGI T'GTAC_A
Cuts at: 0 1930 2247
Size: 1930 317
BssHII G'CGCG_C
Cuts at: 0 1686 2247
Size: 1686 561
BstBI TT'CG_AA
Cuts at: 0 562 2247
Size: 562 1685
BstEII G'GTnAC_C
Cuts at: 0 1980 2247
Size: 1980 267
BstYI r'GATC_y
Cuts at: 0 525 1149 2101 2247
Size: 525 624 952 146
Fragments arranged by size:
952 624 525 146
Bsu36I CC'TnA_GG
Cuts at: 0 836 2204 2247
Size: 836 1368 43
Fragments arranged by size:
1368 836 43
BtgI C'CryG_G
Cuts at: 0 498 658 1283 1355 2247
Size: 498 160 625 72 892
Fragments arranged by size:
892 625 498 160 72
BtsI GCAGTG_nn'
Cuts at: 0 56 2247
Size: 56 2191
EaeI y'GGCC_r
Cuts at: 0 27 710 2247
Size: 27 683 1537
Fragments arranged by size:
1537 683 27
EarI CTCTTCn'nnn_
Cuts at: 0 269 305 316 606 849 916 1010 1232
Size: 269 36 11 290 243 67 94 222
Cuts at: 1232 1450 1508 1556 1769 2247
Size: 218 58 48 213 478
Fragments arranged by size:
478 290 269 243 222 218 213 94
67 58 48 36 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 161 1096 1663 2247
Size: 19 142 935 567 584
Fragments arranged by size:
935 584 567 142 19
EcoICRI GAG'CTC
Cuts at: 0 637 1894 2247
Size: 637 1257 353
Fragments arranged by size:
1257 637 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 611 761 1262 1280 1298 1316 1580
Size: 90 521 150 501 18 18 18 264
Cuts at: 1580 1810 1922 2247
Size: 230 112 325
Fragments arranged by size:
521 501 325 264 230 150 112 90
18 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2203 2247
Size: 2203 44
EcoO109I rG'GnC_Cy
Cuts at: 0 2195 2247
Size: 2195 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2206 2247
Size: 2206 41
HaeII r_GCGC'y
Cuts at: 0 1663 1788 2247
Size: 1663 125 459
Fragments arranged by size:
1663 459 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 803 835 1573 1605 2108 2140
Size: 523 32 248 32 738 32 503 32
Cuts at: 2140 2247
Size: 107
Fragments arranged by size:
738 523 503 248 107 32 32 32
32
HincII GTy'rAC
Cuts at: 0 678 2247
Size: 678 1569
HpaI GTT'AAC
Cuts at: 0 678 2247
Size: 678 1569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 2247
Size: 308 1939
MscI TGG'CCA
Cuts at: 0 712 2247
Size: 712 1535
MslI CAynn'nnrTG
Cuts at: 0 769 1926 2247
Size: 769 1157 321
Fragments arranged by size:
1157 769 321
MspA1I CmG'CkG
Cuts at: 0 201 730 1635 1641 2247
Size: 201 529 905 6 606
Fragments arranged by size:
905 606 529 201 6
NcoI C'CATG_G
Cuts at: 0 498 658 1283 1355 2247
Size: 498 160 625 72 892
Fragments arranged by size:
892 625 498 160 72
NheI G'CTAG_C
Cuts at: 0 240 2247
Size: 240 2007
NspI r_CATG'y
Cuts at: 0 1978 2247
Size: 1978 269
PciI A'CATG_T
Cuts at: 0 1974 2247
Size: 1974 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 1264 2247
Size: 1264 983
PfoI T'CCnGG_A
Cuts at: 0 1944 2247
Size: 1944 303
PsiI TTA'TAA
Cuts at: 0 2086 2247
Size: 2086 161
PspOMI G'GGCC_C
Cuts at: 0 2194 2247
Size: 2194 53
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1827 1859 2201 2233 2247
Size: 1827 32 342 32 14
Fragments arranged by size:
1827 342 32 32 14
PstI C_TGCA'G
Cuts at: 0 650 1413 1640 2238 2247
Size: 650 763 227 598 9
Fragments arranged by size:
763 650 598 227 9
PvuII CAG'CTG
Cuts at: 0 730 1635 2247
Size: 730 905 612
Fragments arranged by size:
905 730 612
SacI G_AGCT'C
Cuts at: 0 639 1896 2247
Size: 639 1257 351
Fragments arranged by size:
1257 639 351
SapI GCTCTTCn'nnn_
Cuts at: 0 269 305 849 1232 1508 1769 2247
Size: 269 36 544 383 276 261 478
Fragments arranged by size:
544 478 383 276 269 261 36
SfcI C'TryA_G
Cuts at: 0 646 1409 1636 1844 2234 2247
Size: 646 763 227 208 390 13
Fragments arranged by size:
763 646 390 227 208 13
SmaI CCC'GGG
Cuts at: 0 211 1618 2247
Size: 211 1407 629
Fragments arranged by size:
1407 629 211
SmlI C'TyrA_G
Cuts at: 0 394 1807 2247
Size: 394 1413 440
Fragments arranged by size:
1413 440 394
StyI C'CwwG_G
Cuts at: 0 498 658 1283 1355 1886 2247
Size: 498 160 625 72 531 361
Fragments arranged by size:
625 531 498 361 160 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1041 1047 2247
Size: 1041 6 1200
Fragments arranged by size:
1200 1041 6
TatI w'GTAC_w
Cuts at: 0 906 1930 2247
Size: 906 1024 317
Fragments arranged by size:
1024 906 317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 689 721 1898 1930 2247
Size: 689 32 1177 32 317
Fragments arranged by size:
1177 689 317 32 32
XbaI T'CTAG_A
Cuts at: 0 793 2247
Size: 793 1454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1353 2247
Size: 1353 894
XmaI C'CCGG_G
Cuts at: 0 209 1616 2247
Size: 209 1407 631
Fragments arranged by size:
1407 631 209
XmnI GAAnn'nnTTC
Cuts at: 0 1762 2247
Size: 1762 485
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BclI BglI BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HpaI MmeI MscI
MslI MspA1I NcoI NheI NspI PciI PflMI PfoI
PsiI PspOMI PsrI PstI PvuII SacI SapI SfcI
SmaI SmlI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglII BmrI
BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BssSI BstAPI BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI
HindIII KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PpuMI PshAI PspXI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XhoI ZraI