Restriction map for pFN21AB6277
 (Linear) (Six-base) MAPSORT of: KIBB6277.seq  Check: 9741  from: 1  to: 2343



KIBB6277.orf

 With 182 enzymes: SgfI * 

                        August 6, 2008 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    2031    2343 
   Size:       1051     980     312

  Fragments arranged by size: 

                1051     980     312
 
AflIII A'CryG_T

Cuts at:      0     448    2343 
   Size:        448    1895
 
AleI CACnn'nnGTG

Cuts at:      0    2162    2231    2343 
   Size:       2162      69     112

  Fragments arranged by size: 

                2162     112      69
 
AlwNI CAG_nnn'CTG

Cuts at:      0     393    1171    1224    1969    2343 
   Size:        393     778      53     745     374

  Fragments arranged by size: 

                 778     745     393     374      53
 
ApaLI G'TGCA_C

Cuts at:      0    1625    2343 
   Size:       1625     718
 
ApoI r'AATT_y

Cuts at:      0     874     905    1265    2343 
   Size:        874      31     360    1078

  Fragments arranged by size: 

                1078     874     360      31
 
AvaI C'yCGr_G

Cuts at:      0     266    1742    2343 
   Size:        266    1476     601

  Fragments arranged by size: 

                1476     601     266
 
BanI G'GyrC_C

Cuts at:      0     112    1591    2101    2343 
   Size:        112    1479     510     242

  Fragments arranged by size: 

                1479     510     242     112
 
BanII G_rGCy'C

Cuts at:      0     273    1728    2343 
   Size:        273    1455     615

  Fragments arranged by size: 

                1455     615     273
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1379    2343 
   Size:       1379     964
 
BbvCI CC'TCA_GC

Cuts at:      0    1898    2343 
   Size:       1898     445
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     165    1629    2343 
   Size:        165    1464     714

  Fragments arranged by size: 

                1464     714     165
 
BglII A'GATC_T

Cuts at:      0    2262    2343 
   Size:       2262      81
 
Bme1580I G_kGCm'C

Cuts at:      0    1629    2106    2343 
   Size:       1629     477     237

  Fragments arranged by size: 

                1629     477     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    2285    2343 
   Size:        722    1563      58

  Fragments arranged by size: 

                1563     722      58
 
Bpu10I CC'TnA_GC

Cuts at:      0    1865    1898    2343 
   Size:       1865      33     445

  Fragments arranged by size: 

                1865     445      33
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    2343 
   Size:        209    2134
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1204    2343 
   Size:       1204    1139
 
BsaAI yAC'GTr

Cuts at:      0     451     598    2343 
   Size:        451     147    1745

  Fragments arranged by size: 

                1745     451     147
 
BsaWI w'CCGG_w

Cuts at:      0    1720    2343 
   Size:       1720     623
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     111     141    2343 
   Size:        111      30    2202

  Fragments arranged by size: 

                2202     111      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563    1904    2343 
   Size:        563    1341     439

  Fragments arranged by size: 

                1341     563     439
 
BseYI C'CCAG_C

Cuts at:      0     643    1640    2343 
   Size:        643     997     703

  Fragments arranged by size: 

                 997     703     643
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1277    1819    2343 
   Size:       1277     542     524

  Fragments arranged by size: 

                1277     542     524
 
BsiHKAI G_wGCw'C

Cuts at:      0     273    1401    1629    2343 
   Size:        273    1128     228     714

  Fragments arranged by size: 

                1128     714     273     228
 
BsiWI C'GTAC_G

Cuts at:      0    1648    2343 
   Size:       1648     695
 
BsmI GAATG_Cn'

Cuts at:      0     707    1091    1452    2343 
   Size:        707     384     361     891

  Fragments arranged by size: 

                 891     707     384     361
 
Bsp1286I G_dGCh'C

Cuts at:      0     273    1401    1629    1728    2106    2343 
   Size:        273    1128     228      99     378     237

  Fragments arranged by size: 

                1128     378     273     237     228      99
 
BsrDI GCAATG_nn'

Cuts at:      0     266    2343 
   Size:        266    2077
 
BsrFI r'CCGG_y

Cuts at:      0    1062    2343 
   Size:       1062    1281
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     442    1570    2343 
   Size:        442    1128     773

  Fragments arranged by size: 

                1128     773     442
 
BstBI TT'CG_AA

Cuts at:      0    1542    2343 
   Size:       1542     801
 
BstEII G'GTnAC_C

Cuts at:      0    1248    2343 
   Size:       1248    1095
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1324    1508    2066    2230    2343 
   Size:       1324     184     558     164     113

  Fragments arranged by size: 

                1324     558     184     164     113
 
BstYI r'GATC_y

Cuts at:      0     323    1113    2262    2343 
   Size:        323     790    1149      81

  Fragments arranged by size: 

                1149     790     323      81
 
Bsu36I CC'TnA_GG

Cuts at:      0     155    2343 
   Size:        155    2188
 
BtgI C'CryG_G

Cuts at:      0      38    1655    2343 
   Size:         38    1617     688

  Fragments arranged by size: 

                1617     688      38
 
BtsI GCAGTG_nn'

Cuts at:      0     821    2343 
   Size:        821    1522
 
EaeI y'GGCC_r

Cuts at:      0      35     686    2172    2220    2343 
   Size:         35     651    1486      48     123

  Fragments arranged by size: 

                1486     651     123      48      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     155    1406    2050    2343 
   Size:        155    1251     644     293

  Fragments arranged by size: 

                1251     644     293     155
 
EcoICRI GAG'CTC

Cuts at:      0     271    2343 
   Size:        271    2072
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    1051    2031    2285    2343 
   Size:        722     329     980     254      58

  Fragments arranged by size: 

                 980     722     329     254      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     647     986    1848    2280    2343 
   Size:        647     339     862     432      63

  Fragments arranged by size: 

                 862     647     432     339      63
 
EcoRI G'AATT_C

Cuts at:      0    1265    2343 
   Size:       1265    1078
 
EcoRV GAT'ATC

Cuts at:      0    2125    2343 
   Size:       2125     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1219    2343 
   Size:       1219    1124
 
HaeII r_GCGC'y

Cuts at:      0    1647    2343 
   Size:       1647     696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     255     287     408     440     545     577    1182    1214 
   Size:        255      32     121      32     105      32     605      32

Cuts at:   1214    1530    1562    2025    2057    2067    2099    2343 
   Size:        316      32     463      32      10      32     244

  Fragments arranged by size: 

                 605     463     316     255     244     121     105      32
                  32      32      32      32      32      32      10
 
HincII GTy'rAC

Cuts at:      0    1123    2343 
   Size:       1123    1220
 
HindIII A'AGCT_T

Cuts at:      0     545     965    1091    2343 
   Size:        545     420     126    1252

  Fragments arranged by size: 

                1252     545     420     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       8    1512    2343 
   Size:          8    1504     831

  Fragments arranged by size: 

                1504     831       8
 
MscI TGG'CCA

Cuts at:      0      37     688    2174    2222    2343 
   Size:         37     651    1486      48     121

  Fragments arranged by size: 

                1486     651     121      48      37
 
MslI CAynn'nnrTG

Cuts at:      0      37     110     671    2162    2231    2343 
   Size:         37      73     561    1491      69     112

  Fragments arranged by size: 

                1491     561     112      73      69      37
 
MspA1I CmG'CkG

Cuts at:      0     643    1261    1803    1966    2319    2343 
   Size:        643     618     542     163     353      24

  Fragments arranged by size: 

                 643     618     542     353     163      24
 
NaeI GCC'GGC

Cuts at:      0    1064    2343 
   Size:       1064    1279
 
NcoI C'CATG_G

Cuts at:      0      38    1655    2343 
   Size:         38    1617     688

  Fragments arranged by size: 

                1617     688      38
 
NdeI CA'TA_TG

Cuts at:      0    1959    2343 
   Size:       1959     384
 
NgoMIV G'CCGG_C

Cuts at:      0    1062    2343 
   Size:       1062    1281
 
NspI r_CATG'y

Cuts at:      0     321     403    2275    2343 
   Size:        321      82    1872      68

  Fragments arranged by size: 

                1872     321      82      68
 
PasI CC'CwG_GG

Cuts at:      0    2106    2343 
   Size:       2106     237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372     498    2251    2343 
   Size:        372     126    1753      92

  Fragments arranged by size: 

                1753     372     126      92
 
PmlI CAC'GTG

Cuts at:      0     451    2343 
   Size:        451    1892
 
PpuMI rG'GwC_Cy

Cuts at:      0     647     986    1848    2280    2343 
   Size:        647     339     862     432      63

  Fragments arranged by size: 

                 862     647     432     339      63
 
PsiI TTA'TAA

Cuts at:      0    1295    2343 
   Size:       1295    1048
 
PspXI vC'TCGA_Gb

Cuts at:      0     266    2343 
   Size:        266    2077
 
PstI C_TGCA'G

Cuts at:      0     516     839    1845    1884    2343 
   Size:        516     323    1006      39     459

  Fragments arranged by size: 

                1006     516     459     323      39
 
PvuII CAG'CTG

Cuts at:      0    1261    1803    1966    2319    2343 
   Size:       1261     542     163     353      24

  Fragments arranged by size: 

                1261     542     353     163      24
 
SacI G_AGCT'C

Cuts at:      0     273    2343 
   Size:        273    2070
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1406    2343 
   Size:       1406     937
 
SexAI A'CCwGG_T

Cuts at:      0     491    2343 
   Size:        491    1852
 
SfcI C'TryA_G

Cuts at:      0     512     835    1841    1880    2343 
   Size:        512     323    1006      39     463

  Fragments arranged by size: 

                1006     512     463     323      39
 
SmlI C'TyrA_G

Cuts at:      0       8     224     266    2343 
   Size:          8     216      42    2077

  Fragments arranged by size: 

                2077     216      42       8
 
SnaBI TAC'GTA

Cuts at:      0     598    2343 
   Size:        598    1745
 
SpeI A'CTAG_T

Cuts at:      0    1605    2343 
   Size:       1605     738
 
SphI G_CATG'C

Cuts at:      0     321    2275    2343 
   Size:        321    1954      68

  Fragments arranged by size: 

                1954     321      68
 
StyI C'CwwG_G

Cuts at:      0      38    1203    1655    2118    2183    2343 
   Size:         38    1165     452     463      65     160

  Fragments arranged by size: 

                1165     463     452     160      65      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1312    1511    1648    2206    2343 
   Size:       1312     199     137     558     137

  Fragments arranged by size: 

                1312     558     199     137     137
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     440     472    1486    1518    1932    1964    2343 
   Size:        440      32    1014      32     414      32     379

  Fragments arranged by size: 

                1014     440     414     379      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1505    2343 
   Size:       1505     838
 
XhoI C'TCGA_G

Cuts at:      0     266    2343 
   Size:        266    2077
 
XmnI GAAnn'nnTTC

Cuts at:      0     105    1269    2343 
   Size:        105    1164    1074

  Fragments arranged by size: 

                1164    1074     105
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbsI    BbvCI    BciVI    BglII Bme1580I     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BsrDI    BsrFI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NspI     PasI    PflMI     PmlI    PpuMI     PsiI
    PspXI     PstI    PvuII     SacI     SapI    SexAI     SfcI     SmlI
    SnaBI     SpeI     SphI     StyI    TaqII     TstI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI    BsaBI    BsaHI    BsiEI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PpiI    PshAI   PspOMI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XmaI     ZraI
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