Restriction map for
pFN21AE0629
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 470 ACACGT A^C[GA][CT]GT
AgeI 523 ACCGGT A^CCGGT
AspHI 124 GAGCTC G[AT]GC[AT]^C
BbvI 489 GCAGC GCAGC(8/12)
BcgI 110 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 523 ACCGGT [AT]^CCGG[AT]
BseRI 79 GAGGAG GAGGAG(10/8)
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
BsmI 228 GCATTC GAATGC(1/-1)
BsrBI 463 CCGCTC CCGCTC(-3/-3)
BsrFI 523 ACCGGT [GA]^CCGG[CT]
BstBI 284 TTCGAA TT^CGAA
BstNI 588 CCAGG CC^[AT]GG
CfoI 100 GCGC GCG^C
Cfr10I 523 ACCGGT [GA]^CCGG[CT]
DpnII 375 GATC ^GATC
DraII 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 63 GACCGCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 65 CCGCGG C^C[GA][CT]GG
EcoO109I 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 588 CCAGG ^CC[AT]GG
HaeIII 114 GGCC GG^CC
HhaI 100 GCGC GCG^C
HinP1I 100 GCGC G^CGC
HindIII 343 AAGCTT A^AGCTT
KspI 65 CCGCGG CCGC^GG
MaeII 472 ACGT A^CGT
MboI 375 GATC ^GATC
MvaI 588 CCAGG CC^[AT]GG
NdeI 327 CATATG CA^TATG
NdeII 375 GATC ^GATC
NspV 284 TTCGAA TT^CGAA
PinAI 523 ACCGGT A^CCGGT
PleI 319 GACTC GAGTC(4/5)
PstI 487 CTGCAG CTGCA^G
PvuII 266 CAGCTG CAG^CTG
SacI 124 GAGCTC GAGCT^C
SacII 65 CCGCGG CCGC^GG
Sau3AI 375 GATC ^GATC
SfaNI 492 GCATC GCATC(5/9)
SfuI 284 TTCGAA TT^CGAA
SmlI 16 CTTGAG C^T[CT][GA]AG
TfiI 287 GAATC G^A[AT]TC
TseI 489 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 307 AAATTC [GA]^AATT[CT]
583 AAATTC
AfaI 144 GTAC GT^AC
521 GTAC
ApoI 307 AAATTC [GA]^AATT[CT]
583 AAATTC
BanII 19 GAGCCC G[GA]GC[CT]^C
124 GAGCTC
BcnI 26 CCGGG CC^[GC]GG
428 CCCGG
BmyI 19 GAGCCC G[AGT]GC[ACT]^C
124 GAGCTC
BsaJI 65 CCGCGG C^C[ACGT][ACGT]GG
427 CCCCGG
BsiYI 90 CCCGCAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
427 CCCCGGAACGG
BslI 90 CCCGCAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
427 CCCCGGAACGG
Bsp1286I 19 GAGCCC G[AGT]GC[ACT]^C
124 GAGCTC
BsrI 146 ACTGG ACTGG(1/-1)
54 CCAGT
BstF5I 86 CATCC GGATG(2/0)
139 GGATG
Cac8I 93 GCAGGC GC[ACGT]^[ACGT]GC
224 GCTCGC
Fnu4HI 97 GCGGC GC^[ACGT]GC
489 GCAGC
FokI 86 CATCC GGATG(9/13)
139 GGATG
HgaI 45 GACGC GACGC(5/10)
102 GCGTC
HinfI 287 GAATC G^A[ACGT]TC
319 GACTC
HphI 444 TCACC GGTGA(8/7)
506 TCACC
ItaI 97 GCGGC GC^[ACGT]GC
489 GCAGC
MboII 574 GAAGA GAAGA(8/7)
282 TCTTC
MspA1I 65 CCGCGG C[AC]G^C[GT]G
266 CAGCTG
NciI 26 CCGGG CC^[GC]GG
428 CCCGG
RsaI 144 GTAC GT^AC
521 GTAC
SfcI 487 CTGCAG C^T[GA][CT]AG
498 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BsmFI BssKI BstUI Cfr13I FauI
HapII HpaII MnlI MseI MspI
MvnI MwoI NlaIV Sau96I ScrFI
TaqI Tru9I Tsp509I TspRI TthHB8I