Restriction map for
pFN21AE3123
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 868 GTAC GT^AC
AflIII 858 ACATGT A^C[GA][CT]GT
AseI 573 ATTAAT AT^TAAT
AsnI 573 ATTAAT AT^TAAT
AvaII 834 GGACC G^G[AT]CC
BamHI 435 GGATCC G^GATCC
BbvI 901 GCAGC GCAGC(8/12)
BciVI 912 GGATAC GTATCC(6/5)
BclI 485 TGATCA T^GATCA
BpmI 140 CTGGAG CTGGAG(16/14)
BseRI 23 GAGGAG GAGGAG(10/8)
BspLU11I 858 ACATGT A^CATGT
BstBI 534 TTCGAA TT^CGAA
BstYI 435 GGATCC [GA]^GATC[CT]
DraI 925 TTTAAA TTT^AAA
DraII 833 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 132 TGGCCG [CT]^GGCC[GA]
EcoO109I 833 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 376 GATATC GAT^ATC
FbaI 485 TGATCA T^GATCA
Fnu4HI 901 GCAGC GC^[ACGT]GC
HapII 313 CCGG C^CGG
HpaII 313 CCGG C^CGG
HphI 342 TCACC GGTGA(8/7)
ItaI 901 GCAGC GC^[ACGT]GC
MaeII 843 ACGT A^CGT
MflI 435 GGATCC [GA]^GATC[CT]
MspA1I 805 CAGCTG C[AC]G^C[GT]G
MspI 313 CCGG C^CGG
MwoI 651 GCTAAATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 858 ACATGT [GA]CATG^[CT]
NspV 534 TTCGAA TT^CGAA
PpuMI 833 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 573 ATTAAT AT^TAAT
PstI 68 CTGCAG CTGCA^G
PvuII 805 CAGCTG CAG^CTG
RsaI 868 GTAC GT^AC
SfuI 534 TTCGAA TT^CGAA
SspI 883 AATATT AAT^ATT
TaqI 535 TCGA T^CGA
TseI 901 GCAGC G^C[AT]GC
TthHB8I 535 TCGA T^CGA
XcmI 839 CCAAACGTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 435 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 178 GAATTT [GA]^AATT[CT]
654 AAATTT
ApoI 178 GAATTT [GA]^AATT[CT]
654 AAATTT
BstF5I 674 GGATG GGATG(2/0)
864 GGATG
Cac8I 807 GCTGGC GC[ACGT]^[ACGT]GC
827 GCAAGC
EarI 99 CTCTTC CTCTTC(1/4)
666 GAAGAG
FokI 674 GGATG GGATG(9/13)
864 GGATG
HinfI 537 GAATC G^A[ACGT]TC
724 GAATC
Ksp632I 99 CTCTTC CTCTTC(1/4)
666 GAAGAG
MaeIII 341 GTCAC ^GT[ACGT]AC
545 GTCAC
MslI 130 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
859 CATGTGGATG
TfiI 537 GAATC G^A[AT]TC
724 GAATC
Tsp45I 341 GTCAC ^GT[GC]AC
545 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsrI BssKI BstNI Cfr13I DdeI
DpnII EcoRII HaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I Tsp509I TspRI