(Linear) (Six-base) MAPSORT of: KIEE9394.seq Check: 2629 from: 1 to: 3489 KIEE9394.orf With 182 enzymes: SgfI * December 27, 2011 16:38 .. AccI GT'mk_AC Cuts at: 0 1769 2819 3489 Size: 1769 1050 670 Fragments arranged by size: 1769 1050 670 Acc65I G'GTAC_C Cuts at: 0 2132 3489 Size: 2132 1357 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1454 1745 1765 3067 3085 3115 3227 Size: 880 574 291 20 1302 18 30 112 Cuts at: 3227 3489 Size: 262 Fragments arranged by size: 1302 880 574 291 262 112 30 20 18 AhdI GACnn_n'nnGTC Cuts at: 0 459 2504 3489 Size: 459 2045 985 Fragments arranged by size: 2045 985 459 AleI CACnn'nnGTG Cuts at: 0 455 3489 Size: 455 3034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2429 3489 Size: 2429 1060 AlwNI CAG_nnn'CTG Cuts at: 0 928 1723 3453 3489 Size: 928 795 1730 36 Fragments arranged by size: 1730 928 795 36 ApaI G_GGCC'C Cuts at: 0 3397 3489 Size: 3397 92 ApoI r'AATT_y Cuts at: 0 59 276 1030 2361 3099 3323 3364 3489 Size: 59 217 754 1331 738 224 41 125 Fragments arranged by size: 1331 754 738 224 217 125 59 41 AvaI C'yCGr_G Cuts at: 0 638 1384 2005 3489 Size: 638 746 621 1484 Fragments arranged by size: 1484 746 638 621 BanI G'GyrC_C Cuts at: 0 1118 2132 2961 3489 Size: 1118 1014 829 528 Fragments arranged by size: 1118 1014 829 528 BanII G_rGCy'C Cuts at: 0 3397 3489 Size: 3397 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 17 80 1175 1847 2504 2602 2614 3489 Size: 17 63 1095 672 657 98 12 875 Fragments arranged by size: 1095 875 672 657 98 63 17 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2470 2504 3445 3479 3489 Size: 2470 34 941 34 10 Fragments arranged by size: 2470 941 34 34 10 BclI T'GATC_A Cuts at: 0 600 3489 Size: 600 2889 BfrBI ATG'CAT Cuts at: 0 919 2791 2863 3489 Size: 919 1872 72 626 Fragments arranged by size: 1872 919 626 72 BglII A'GATC_T Cuts at: 0 205 3426 3489 Size: 205 3221 63 Fragments arranged by size: 3221 205 63 Bme1580I G_kGCm'C Cuts at: 0 1121 1531 3397 3489 Size: 1121 410 1866 92 Fragments arranged by size: 1866 1121 410 92 BmrI ACTGGGnnnn_n' Cuts at: 0 666 1541 3489 Size: 666 875 1948 Fragments arranged by size: 1948 875 666 BmtI G_CTAG'C Cuts at: 0 1781 3489 Size: 1781 1708 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1397 1994 2270 2502 3489 Size: 1397 597 276 232 987 Fragments arranged by size: 1397 987 597 276 232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1097 1254 1776 3489 Size: 311 786 157 522 1713 Fragments arranged by size: 1713 786 522 311 157 Bpu10I CC'TnA_GC Cuts at: 0 2879 3489 Size: 2879 610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1481 1806 3101 3489 Size: 1481 325 1295 388 Fragments arranged by size: 1481 1295 388 325 BsaI GGTCTCn'nnnn_ Cuts at: 0 593 3489 Size: 593 2896 BsaHI Gr'CG_yC Cuts at: 0 77 3489 Size: 77 3412 BsaWI w'CCGG_w Cuts at: 0 14 3489 Size: 14 3475 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1837 1867 2560 2590 3489 Size: 1837 30 693 30 899 Fragments arranged by size: 1837 899 693 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 86 1224 2025 2646 3489 Size: 86 1138 801 621 843 Fragments arranged by size: 1138 843 801 621 86 BseYI C'CCAG_C Cuts at: 0 343 865 2446 3489 Size: 343 522 1581 1043 Fragments arranged by size: 1581 1043 522 343 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1634 2660 3439 3489 Size: 1634 1026 779 50 Fragments arranged by size: 1634 1026 779 50 BsiEI CG_ry'CG Cuts at: 0 456 3489 Size: 456 3033 BsiHKAI G_wGCw'C Cuts at: 0 821 3092 3489 Size: 821 2271 397 Fragments arranged by size: 2271 821 397 BsmI GAATG_Cn' Cuts at: 0 972 2865 2880 3489 Size: 972 1893 15 609 Fragments arranged by size: 1893 972 609 15 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2542 2908 3489 Size: 2542 366 581 Fragments arranged by size: 2542 581 366 Bsp1286I G_dGCh'C Cuts at: 0 821 1121 1531 3092 3397 3489 Size: 821 300 410 1561 305 92 Fragments arranged by size: 1561 821 410 305 300 92 BspHI T'CATG_A Cuts at: 0 3419 3489 Size: 3419 70 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 403 3489 Size: 403 3086 BssSI C'ACGA_G Cuts at: 0 1733 2244 3489 Size: 1733 511 1245 Fragments arranged by size: 1733 1245 511 BstAPI GCAn_nnn'nTGC Cuts at: 0 2968 3453 3489 Size: 2968 485 36 Fragments arranged by size: 2968 485 36 BstXI CCAn_nnnn'nTGG Cuts at: 0 838 3489 Size: 838 2651 BstYI r'GATC_y Cuts at: 0 205 1297 1315 2466 2922 3263 3426 3489 Size: 205 1092 18 1151 456 341 163 63 Fragments arranged by size: 1151 1092 456 341 205 163 63 18 BstZ17I GTA'TAC Cuts at: 0 2820 3489 Size: 2820 669 Bsu36I CC'TnA_GG Cuts at: 0 372 3031 3489 Size: 372 2659 458 Fragments arranged by size: 2659 458 372 BtgI C'CryG_G Cuts at: 0 776 794 837 3489 Size: 776 18 43 2652 Fragments arranged by size: 2652 776 43 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3024 3110 3489 Size: 3024 86 379 Fragments arranged by size: 3024 379 86 BtsI GCAGTG_nn' Cuts at: 0 758 1170 3489 Size: 758 412 2319 Fragments arranged by size: 2319 758 412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 704 739 3489 Size: 704 35 2750 Fragments arranged by size: 2750 704 35 DraIII CAC_nnn'GTG Cuts at: 0 1657 3489 Size: 1657 1832 EarI CTCTTCn'nnn_ Cuts at: 0 1473 1721 2084 2488 3489 Size: 1473 248 363 404 1001 Fragments arranged by size: 1473 1001 404 363 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 63 3489 Size: 63 3426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 880 1097 1254 1454 1745 1765 1776 Size: 311 569 217 157 200 291 20 11 Cuts at: 1776 3067 3085 3115 3227 3489 Size: 1291 18 30 112 262 Fragments arranged by size: 1291 569 311 291 262 217 200 157 112 30 20 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1736 3489 Size: 1736 1753 EcoO109I rG'GnC_Cy Cuts at: 0 334 642 3394 3489 Size: 334 308 2752 95 Fragments arranged by size: 2752 334 308 95 EcoRI G'AATT_C Cuts at: 0 2361 3099 3364 3489 Size: 2361 738 265 125 Fragments arranged by size: 2361 738 265 125 EcoRV GAT'ATC Cuts at: 0 3485 3489 Size: 3485 4 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1922 2736 2973 3257 3489 Size: 1922 814 237 284 232 Fragments arranged by size: 1922 814 284 237 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 127 159 250 282 445 477 1837 1869 Size: 127 32 91 32 163 32 1360 32 Cuts at: 1869 1930 1962 2490 2522 2779 2811 2907 2939 Size: 61 32 528 32 257 32 96 32 Cuts at: 2939 3489 Size: 550 Fragments arranged by size: 1360 550 528 257 163 127 96 91 61 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1895 3489 Size: 1895 1594 HindIII A'AGCT_T Cuts at: 0 217 2713 3489 Size: 217 2496 776 Fragments arranged by size: 2496 776 217 KpnI G_GTAC'C Cuts at: 0 2136 3489 Size: 2136 1353 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 2306 3326 3489 Size: 1066 1240 1020 163 Fragments arranged by size: 1240 1066 1020 163 MslI CAynn'nnrTG Cuts at: 0 455 596 1250 2306 3489 Size: 455 141 654 1056 1183 Fragments arranged by size: 1183 1056 654 455 141 MspA1I CmG'CkG Cuts at: 0 402 1720 2446 2977 3489 Size: 402 1318 726 531 512 Fragments arranged by size: 1318 726 531 512 402 NcoI C'CATG_G Cuts at: 0 776 794 837 3489 Size: 776 18 43 2652 Fragments arranged by size: 2652 776 43 18 NheI G'CTAG_C Cuts at: 0 1777 3489 Size: 1777 1712 NsiI A_TGCA'T Cuts at: 0 921 2793 2865 3489 Size: 921 1872 72 624 Fragments arranged by size: 1872 921 624 72 NspI r_CATG'y Cuts at: 0 1788 3489 Size: 1788 1701 PflMI CCAn_nnn'nTGG Cuts at: 0 912 3130 3273 3489 Size: 912 2218 143 216 Fragments arranged by size: 2218 912 216 143 PfoI T'CCnGG_A Cuts at: 0 1584 3489 Size: 1584 1905 PpuMI rG'GwC_Cy Cuts at: 0 334 642 3489 Size: 334 308 2847 Fragments arranged by size: 2847 334 308 PshAI GACnn'nnGTC Cuts at: 0 789 3489 Size: 789 2700 PsiI TTA'TAA Cuts at: 0 3107 3489 Size: 3107 382 PspOMI G'GGCC_C Cuts at: 0 3393 3489 Size: 3393 96 PstI C_TGCA'G Cuts at: 0 2982 3489 Size: 2982 507 PvuII CAG'CTG Cuts at: 0 1720 2977 3489 Size: 1720 1257 512 Fragments arranged by size: 1720 1257 512 SanDI GG'GwC_CC Cuts at: 0 642 3489 Size: 642 2847 SapI GCTCTTCn'nnn_ Cuts at: 0 1721 3489 Size: 1721 1768 ScaI AGT'ACT Cuts at: 0 2999 3070 3489 Size: 2999 71 419 Fragments arranged by size: 2999 419 71 SfcI C'TryA_G Cuts at: 0 239 822 1682 1766 1868 2972 2978 3489 Size: 239 583 860 84 102 1104 6 511 Fragments arranged by size: 1104 860 583 511 239 102 84 6 SmlI C'TyrA_G Cuts at: 0 1496 1821 3116 3489 Size: 1496 325 1295 373 Fragments arranged by size: 1496 1295 373 325 SphI G_CATG'C Cuts at: 0 1788 3489 Size: 1788 1701 StuI AGG'CCT Cuts at: 0 1742 3489 Size: 1742 1747 StyI C'CwwG_G Cuts at: 0 776 794 837 2210 2624 3489 Size: 776 18 43 1373 414 865 Fragments arranged by size: 1373 865 776 414 43 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 357 2614 3489 Size: 357 2257 875 Fragments arranged by size: 2257 875 357 TatI w'GTAC_w Cuts at: 0 1213 2997 3068 3489 Size: 1213 1784 71 421 Fragments arranged by size: 1784 1213 421 71 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3248 3280 3489 Size: 3248 32 209 Fragments arranged by size: 3248 209 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1409 3489 Size: 1409 2080 XmnI GAAnn'nnTTC Cuts at: 0 190 2736 3204 3489 Size: 190 2546 468 285 Fragments arranged by size: 2546 468 285 190 Enzymes that do cut: AccI Acc65I AcuI AhdI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BclI BfrBI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MmeI MslI MspA1I NcoI NheI NsiI NspI PflMI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SanDI SapI ScaI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BglI BlpI BmgBI BsaAI BsaBI BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII ClaI DraI DrdI EaeI EagI EcoICRI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI PacI PasI PciI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI