Restriction map for
pFN21AB9773
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbeI BbrPI BbsI
BbvCI BciVI BfaI BfrI BlnI
BpuAI BsaI BsaWI BseAI BsiWI
Bsp1407I BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MaeI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuII RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XbaI
XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Asp700I 714 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 788 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BcgI 1434 CGACATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1108 GCCTACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1380 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1380 GCTGAGC GC^T[ACGT]AGC
BsaAI 121 TACGTG [CT]AC^GT[GA]
BsmBI 1210 GAGACG CGTCTC(1/5)
BsmI 337 GCATTC GAATGC(1/-1)
BspDI 1438 ATCGAT AT^CGAT
BsrI 266 ACTGG ACTGG(1/-1)
BssSI 1734 CTCGTG CACGAG(-5/-1)
CelII 1380 GCTGAGC GC^T[ACGT]AGC
ClaI 1438 ATCGAT AT^CGAT
DraIII 718 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 816 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1647 GAAGAG CTCTTC(1/4)
FspI 788 TGCGCA TGC^GCA
Ksp632I 1647 GAAGAG CTCTTC(1/4)
KspI 11 CCGCGG CCGC^GG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 438 GCCGGC GCC^GGC
NcoI 362 CCATGG C^CATGG
NgoMIV 438 GCCGGC G^CCGGC
PflMI 717 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1714 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1048 CTGCAG CTGCA^G
SacII 11 CCGCGG CCGC^GG
SapI 1647 GAAGAGC GCTCTTC(1/4)
SfcI 1048 CTGCAG C^T[GA][CT]AG
SmlI 1672 CTCAAG C^T[CT][GA]AG
TfiI 990 GATTC G^A[AT]TC
TspRI 265 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 717 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 714 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 967 GACGCC G[GA]^CG[CT]C
1744 GACGCC
AflIII 617 ACGTGT A^C[GA][CT]GT
881 ACACGT
AlwI 1317 GATCC GGATC(4/5)
512 GGATC
AlwNI 1039 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1165 CAGGACCTG
AspI 816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
997 GACCTGGTC
BglII 353 AGATCT A^GATCT
1277 AGATCT
BsaBI 18 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1500 GATCACCATC
BsaHI 967 GACGCC G[GA]^CG[CT]C
1744 GACGCC
BsmAI 293 GAGAC GTCTC(1/5)
1210 GAGAC
BsrBI 142 GAGCGG CCGCTC(-3/-3)
1383 GAGCGG
BstEII 414 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
BstPI 414 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
BstYI 353 AGATCT [GA]^GATC[CT]
1277 AGATCT
EcoO65I 414 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
Hin1I 967 GACGCC G[GA]^CG[CT]C
1744 GACGCC
MamI 18 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1500 GATCACCATC
MflI 353 AGATCT [GA]^GATC[CT]
1277 AGATCT
MslI 679 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1134 CATCCTGATG
MspA1I 11 CCGCGG C[AC]G^C[GT]G
1821 CAGCGG
PflFI 816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
997 GACCTGGTC
PvuI 17 CGATCG CGAT^CG
632 CGATCG
SexAI 170 ACCAGGT A^CC[AT]GGT
998 ACCTGGT
SmaI 1306 CCCGGG CCC^GGG
1849 CCCGGG
Tth111I 816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
997 GACCTGGTC
XhoII 353 AGATCT [GA]^GATC[CT]
1277 AGATCT
XmaCI 1306 CCCGGG C^CCGGG
1849 CCCGGG
XmaI 1306 CCCGGG C^CCGGG
1849 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BclI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TthHB8I XcmI