(Circular) (Six-base) MAPSORT of: pFN21AB9773.seq Check: 8764 from: 1 to: 6628 pFN21AB9773 6628 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2305 3796 2305 Size: 1491 5137 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 3949 3949 Size: 6628 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4691 6075 6343 1632 Size: 3059 1384 268 1917 Fragments arranged by size: 3059 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 2622 2886 5527 6504 1203 Size: 1419 264 2641 977 1327 Fragments arranged by size: 2641 1419 1327 977 264 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 2688 1166 Size: 1522 5106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6628 AlwNI CAG_nnn'CTG Cuts at: 3049 3175 4371 5943 3049 Size: 126 1196 1572 3734 Fragments arranged by size: 3734 1572 1196 126 ApaLI G'TGCA_C Cuts at: 4685 5841 4685 Size: 1156 5472 ApoI r'AATT_y Cuts at: 1493 3937 4163 4315 6424 1493 Size: 2444 226 152 2109 1697 Fragments arranged by size: 2444 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 2351 3311 3854 3953 1870 Size: 73 408 960 543 99 4545 Fragments arranged by size: 4545 960 543 408 99 73 BamHI G'GATC_C Cuts at: 3958 4325 3958 Size: 367 6261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2612 3842 3949 5404 Size: 325 217 627 252 573 1230 107 1455 Cuts at: 5404 618 Size: 1842 Fragments arranged by size: 1842 1455 1230 627 573 325 252 217 107 BanII G_rGCy'C Cuts at: 1391 2505 2532 2952 3023 3678 3877 3947 1391 Size: 1114 27 420 71 655 199 70 4072 Fragments arranged by size: 4072 1114 655 420 199 71 70 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3428 3462 4819 4853 1903 Size: 34 1491 34 1357 34 3678 Fragments arranged by size: 3678 1491 1357 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 2286 2433 3243 1548 Size: 738 147 810 4933 Fragments arranged by size: 4933 810 738 147 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3119 5239 136 Size: 107 122 71 631 605 1447 2120 1525 Fragments arranged by size: 2120 1525 1447 631 605 122 107 71 BglII A'GATC_T Cuts at: 2358 3282 6623 2358 Size: 924 3341 2363 Fragments arranged by size: 3341 2363 924 BlpI GC'TnA_GC Cuts at: 3386 4045 3386 Size: 659 5969 Bme1580I G_kGCm'C Cuts at: 1790 2042 2615 3420 4689 5845 1790 Size: 252 573 805 1269 1156 2573 Fragments arranged by size: 2573 1269 1156 805 573 252 BmgBI CAC'GTC Cuts at: 1318 2623 2889 3190 1318 Size: 1305 266 301 4756 Fragments arranged by size: 4756 1305 301 266 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4359 5317 442 Size: 416 53 83 399 24 2942 958 1753 Fragments arranged by size: 2942 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2377 2875 2938 3754 2377 Size: 498 63 816 5251 Fragments arranged by size: 5251 816 498 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2870 2966 3233 3650 3821 5288 1239 Size: 1631 96 267 417 171 1467 2579 Fragments arranged by size: 2579 1631 1467 417 267 171 96 Bpu10I CC'TnA_GC Cuts at: 3533 3876 3533 Size: 343 6285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3662 4753 5618 5916 3662 Size: 1091 865 298 4374 Fragments arranged by size: 4374 1091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5291 882 Size: 617 3792 2219 Fragments arranged by size: 3792 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2128 4419 493 Size: 711 924 2291 2702 Fragments arranged by size: 2702 2291 924 711 BsaBI GATnn'nnATC Cuts at: 2027 3509 6622 2027 Size: 1482 3113 2033 Fragments arranged by size: 3113 2033 1482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2973 3750 4435 4817 Size: 53 83 186 936 1440 777 685 382 Cuts at: 4817 5452 275 Size: 635 1451 Fragments arranged by size: 1451 1440 936 777 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 3794 3824 1178 Size: 30 165 30 945 30 1416 30 3982 Fragments arranged by size: 3982 1416 945 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2747 2927 3557 3932 1186 Size: 1183 378 180 630 375 3882 Fragments arranged by size: 3882 1183 630 378 375 180 BseYI C'CCAG_C Cuts at: 2120 3205 5831 6327 2120 Size: 1085 2626 496 2421 Fragments arranged by size: 2626 2421 1085 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 2333 3062 3149 3188 3581 3617 Size: 573 537 60 729 87 39 393 36 Cuts at: 3617 1163 Size: 4174 Fragments arranged by size: 4174 729 573 537 393 87 60 39 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2230 2538 2640 2673 3222 Size: 275 186 24 205 308 102 33 549 Cuts at: 3222 4078 4839 5867 1540 Size: 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 549 308 275 205 186 102 33 24 BsiHKAI G_wGCw'C Cuts at: 2592 2924 3488 3786 4689 4774 5845 2592 Size: 332 564 298 903 85 1071 3375 Fragments arranged by size: 3375 1071 903 564 332 298 85 BsmI GAATG_Cn' Cuts at: 2342 4139 4232 2342 Size: 1797 93 4738 Fragments arranged by size: 4738 1797 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3209 3209 Size: 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2505 2532 2592 2615 2924 2952 Size: 399 252 463 27 60 23 309 28 Cuts at: 2952 3023 3420 3488 3678 3786 3877 3947 4689 Size: 71 397 68 190 108 91 70 742 Cuts at: 4689 4774 5845 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 399 397 309 252 190 108 91 85 71 70 68 60 28 27 23 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 4517 6247 4517 Size: 1730 4898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 3183 3796 3983 844 Size: 1461 878 613 187 3489 Fragments arranged by size: 3489 1461 878 613 187 BsrBI CCG'CTC Cuts at: 1394 1823 2149 3390 4515 1394 Size: 429 326 1241 1125 3507 Fragments arranged by size: 3507 1241 1125 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 5123 5297 65 Size: 1183 3875 174 1396 Fragments arranged by size: 3875 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2443 3488 3845 5272 5459 1700 Size: 110 633 1045 357 1427 187 2869 Fragments arranged by size: 2869 1427 1045 633 357 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 3739 4688 5700 3739 Size: 949 1012 4667 Fragments arranged by size: 4667 1012 949 BstAPI GCAn_nnn'nTGC Cuts at: 794 2802 3049 3175 794 Size: 2008 247 126 4247 Fragments arranged by size: 4247 2008 247 126 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 2419 3430 1184 Size: 1235 1011 4382 Fragments arranged by size: 4382 1235 1011 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2690 3145 3760 2128 Size: 562 455 615 4996 Fragments arranged by size: 4996 615 562 455 BstYI r'GATC_y Cuts at: 1972 2358 3282 3958 4325 4710 4727 6179 6265 Size: 386 924 676 367 385 17 1452 86 Cuts at: 6265 6277 6623 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 924 676 386 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2367 2532 2667 2952 3474 Size: 552 939 12 351 165 135 285 522 Cuts at: 3474 3660 513 Size: 186 3481 Fragments arranged by size: 3481 939 552 522 351 285 186 165 135 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2074 2530 487 Size: 683 56 848 456 4585 Fragments arranged by size: 4585 848 683 456 56 BtsI GCAGTG_nn' Cuts at: 4144 4938 4958 4144 Size: 794 20 5814 Fragments arranged by size: 5814 794 20 ClaI AT'CG_AT Cuts at: 1554 3444 1554 Size: 1890 4738 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DraIII CAC_nnn'GTG Cuts at: 2728 2728 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2827 5635 809 Size: 507 1511 2808 1802 Fragments arranged by size: 2808 1802 1511 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2166 2227 Size: 54 1425 50 275 18 177 159 61 Cuts at: 2227 2535 2670 3135 3219 3471 3762 4075 4964 Size: 308 135 465 84 252 291 313 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 465 313 308 291 275 252 177 159 135 84 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2227 2535 2670 3219 4075 1537 Size: 275 415 308 135 549 856 4090 Fragments arranged by size: 4090 856 549 415 308 275 135 EarI CTCTTCn'nnn_ Cuts at: 1353 3647 4558 1353 Size: 2294 911 3423 Fragments arranged by size: 3423 2294 911 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3584 5215 5589 5735 387 Size: 1064 138 1995 1631 374 146 1280 Fragments arranged by size: 1995 1631 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2870 2966 3233 3650 3821 4691 5288 Size: 393 1238 96 267 417 171 870 597 Cuts at: 5288 6075 6343 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1238 870 787 597 417 393 268 267 171 96 EcoNI CCTnn'n_nnAGG Cuts at: 2806 2867 3536 2806 Size: 61 669 5898 Fragments arranged by size: 5898 669 61 EcoO109I rG'GnC_Cy Cuts at: 2820 3172 3881 3905 2820 Size: 352 709 24 5543 Fragments arranged by size: 5543 709 352 24 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FspI TGC'GCA Cuts at: 2795 5134 2795 Size: 2339 4289 HaeII r_GCGC'y Cuts at: 1678 2186 2470 3379 3660 5775 1678 Size: 508 284 909 281 2115 2531 Fragments arranged by size: 2531 2115 909 508 284 281 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 3596 3628 5269 Size: 32 135 32 776 32 1216 32 1641 Cuts at: 5269 5301 5343 5375 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1641 1216 776 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3972 4218 669 Size: 988 281 2034 246 3079 Fragments arranged by size: 3079 2034 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 3953 3953 Size: 6628 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2092 2238 2949 3324 5742 5926 1305 Size: 787 146 711 375 2418 184 2007 Fragments arranged by size: 2418 2007 787 711 375 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4629 Fragments arranged by size: 4629 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 3143 4586 4945 5104 518 Size: 648 1522 455 1443 359 159 2042 Fragments arranged by size: 2042 1522 1443 648 455 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 3828 4044 4721 5869 6114 1606 Size: 412 1810 216 677 1148 245 2120 Fragments arranged by size: 2120 1810 1148 677 412 245 216 NaeI GCC'GGC Cuts at: 2445 2445 Size: 6628 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 4774 Fragments arranged by size: 4774 939 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NgoMIV G'CCGG_C Cuts at: 2443 2443 Size: 6628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 3986 5531 1132 Size: 97 2757 1545 2229 Fragments arranged by size: 2757 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3408 1348 Size: 2060 4568 PciI A'CATG_T Cuts at: 5527 5527 Size: 6628 PflMI CCAn_nnn'nTGG Cuts at: 1108 2728 1108 Size: 1620 5008 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2820 3172 3881 2820 Size: 352 709 5567 Fragments arranged by size: 5567 709 352 PshAI GACnn'nnGTC Cuts at: 1613 3723 1613 Size: 2110 4518 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PstI C_TGCA'G Cuts at: 830 3057 3980 830 Size: 2227 923 3478 Fragments arranged by size: 3478 2227 923 PvuI CG_AT'CG Cuts at: 2001 2025 2640 2001 Size: 24 615 5989 Fragments arranged by size: 5989 615 24 SacII CC_GC'GG Cuts at: 2019 2019 Size: 6628 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SanDI GG'GwC_CC Cuts at: 3881 3881 Size: 6628 SapI GCTCTTCn'nnn_ Cuts at: 3647 3647 Size: 6628 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SexAI A'CCwGG_T Cuts at: 2175 3003 2175 Size: 828 5800 SfcI C'TryA_G Cuts at: 826 1045 3053 3976 5111 5792 5983 826 Size: 219 2008 923 1135 681 191 1471 Fragments arranged by size: 2008 1471 1135 923 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3313 3856 3955 1872 Size: 1441 543 99 4545 Fragments arranged by size: 4545 1441 543 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 3677 4732 5633 5895 820 Size: 197 926 1734 1055 901 262 1553 Fragments arranged by size: 1734 1553 1055 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6628 StyI C'CwwG_G Cuts at: 513 1065 2004 2307 2337 2367 2478 3036 3759 Size: 552 939 303 30 30 111 558 723 Cuts at: 3759 3885 4067 513 Size: 126 182 3074 Fragments arranged by size: 3074 939 723 558 552 303 182 126 111 30 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2678 2696 2852 4655 4672 4825 5010 Size: 1255 1098 18 156 1803 17 153 185 Cuts at: 5010 5455 325 Size: 445 1498 Fragments arranged by size: 1803 1498 1255 1098 445 185 156 153 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3576 4874 6586 Size: 274 80 33 51 1443 1599 1298 1712 Cuts at: 6586 96 Size: 138 Fragments arranged by size: 1712 1599 1443 1298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3794 3826 6588 6620 3794 Size: 32 2762 32 3802 Fragments arranged by size: 3802 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2824 3005 1316 Size: 1508 181 4939 Fragments arranged by size: 4939 1508 181 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 3625 3845 2176 Size: 1449 220 4959 Fragments arranged by size: 4959 1449 220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3311 3854 3953 1870 Size: 1441 543 99 4545 Fragments arranged by size: 4545 1441 543 99 XmnI GAAnn'nnTTC Cuts at: 2723 4757 2723 Size: 2034 4594 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SfiI SfoI SrfI SwaI