################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AclI AgeI Alw44I ApaLI AscI Asp718I AviII AvrII BbeI BbrPI BlnI BsiEI BsiWI BsmBI BspHI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI EagI EclXI Eco52I FseI FspI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcyI 6754 GACGCC G[GA]^CG[CT]C Aor51HI 6374 AGCGCT AGC^GCT ApaI 1488 GGGCCC GGGCC^C AseI 4317 ATTAAT AT^TAAT AsnI 4317 ATTAAT AT^TAAT BsaHI 6754 GACGCC G[GA]^CG[CT]C BsmI 4565 GAATGC GAATGC(1/-1) Bsp1407I 1335 TGTACA T^GTACA BspDI 4679 ATCGAT AT^CGAT BsrFI 175 ACCGGC [GA]^CCGG[CT] BsrGI 1335 TGTACA T^GTACA BstAPI 1233 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 5744 CCACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 175 ACCGGC [GA]^CCGG[CT] ClaI 4679 ATCGAT AT^CGAT DraIII 1092 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1000 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 6374 AGCGCT AGC^GCT EcoRI 6765 GAATTC G^AATTC EcoT22I 5499 ATGCAT ATGCA^T Hin1I 6754 GACGCC G[GA]^CG[CT]C KspI 25 CCGCGG CCGC^GG NcoI 6578 CCATGG C^CATGG NdeI 4040 CATATG CA^TATG NsiI 5499 ATGCAT ATGCA^T PaeR7I 476 CTCGAG C^TCGAG PshAI 1860 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4317 ATTAAT AT^TAAT SacII 25 CCGCGG CCGC^GG SalI 4408 GTCGAC G^TCGAC SapI 1632 GAAGAGC GCTCTTC(1/4) SexAI 5055 ACCTGGT A^CC[AT]GGT SspBI 1335 TGTACA T^GTACA SspI 3812 AATATT AAT^ATT XhoI 476 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 6455 TCCGGA T^CCGGA 6706 TCCGGA AflII 2125 CTTAAG C^TTAAG 3048 CTTAAG AspI 3972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6220 GACGCAGTC BamHI 4722 GGATCC G^GATCC 5109 GGATCC BcgI 729 CGAGAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2016 GCAGAAGATTCG BciVI 1445 GTATCC GTATCC(6/5) 2535 GTATCC BfrI 2125 CTTAAG C^TTAAG 3048 CTTAAG BglII 3025 AGATCT A^GATCT 3075 AGATCT BlpI 3256 GCTGAGC GC^T[ACGT]AGC 3277 GCTAAGC Bpu1102I 3256 GCTGAGC GC^T[ACGT]AGC 3277 GCTAAGC BsaAI 1480 TACGTG [CT]AC^GT[GA] 4750 TACGTG BseAI 6455 TCCGGA T^CCGGA 6706 TCCGGA BspEI 6455 TCCGGA T^CCGGA 6706 TCCGGA BstEII 87 GGTGACC G^GT[ACGT]ACC 5129 GGTTACC BstPI 87 GGTGACC G^GT[ACGT]ACC 5129 GGTTACC CelII 3256 GCTGAGC GC^T[ACGT]AGC 3277 GCTAAGC EcoNI 785 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5227 CCTGAAACAGG EcoO65I 87 GGTGACC G^GT[ACGT]ACC 5129 GGTTACC EcoRV 4004 GATATC GAT^ATC 6589 GATATC MfeI 2153 CAATTG C^AATTG 2659 CAATTG MroI 6455 TCCGGA T^CCGGA 6706 TCCGGA MunI 2153 CAATTG C^AATTG 2659 CAATTG PflFI 3972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6220 GACGCAGTC ScaI 1583 AGTACT AGT^ACT 2213 AGTACT SmaI 1491 CCCGGG CCC^GGG 4652 CCCGGG Tth111I 3972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6220 GACGCAGTC XbaI 3288 TCTAGA T^CTAGA 3729 TCTAGA XmaCI 1491 CCCGGG C^CCGGG 4652 CCCGGG XmaI 1491 CCCGGG C^CCGGG 4652 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI