(Circular) (Six-base) MAPSORT of: pFN21AE0554.seq Check: 8142 from: 1 to: 5425 pFN21AE0554 5425 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2262 2262 Size: 5425 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2746 2746 Size: 5425 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3488 4872 5140 1632 Size: 1856 1384 268 1917 Fragments arranged by size: 1917 1856 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 2479 4324 5301 1203 Size: 1276 1845 977 1327 Fragments arranged by size: 1845 1327 1276 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 2418 1166 Size: 1252 4173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2105 2513 1184 Size: 921 408 4096 Fragments arranged by size: 4096 921 408 AlwNI CAG_nnn'CTG Cuts at: 2031 2311 2376 3168 4740 2031 Size: 280 65 792 1572 2716 Fragments arranged by size: 2716 1572 792 280 65 ApaI G_GGCC'C Cuts at: 2233 2233 Size: 5425 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2346 2750 1870 Size: 73 112 291 404 4545 Fragments arranged by size: 4545 404 291 112 73 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2746 4201 618 Size: 325 217 627 959 1455 1842 Fragments arranged by size: 1842 1455 959 627 325 217 BanII G_rGCy'C Cuts at: 1391 2233 2572 2744 1391 Size: 842 339 172 4072 Fragments arranged by size: 4072 842 339 172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2373 928 Size: 971 474 3980 Fragments arranged by size: 3980 971 474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3616 3650 1903 Size: 34 1679 34 3678 Fragments arranged by size: 3678 1679 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2722 3317 4527 2722 Size: 595 1210 3620 Fragments arranged by size: 3620 1210 595 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 2684 4036 Size: 107 122 71 631 605 405 607 1352 Cuts at: 4036 136 Size: 1525 Fragments arranged by size: 1525 1352 631 607 605 405 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2233 3486 4642 1790 Size: 443 1253 1156 2573 Fragments arranged by size: 2573 1253 1156 443 BmgBI CAC'GTC Cuts at: 1318 2480 1318 Size: 1162 4263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2566 2708 3156 Size: 416 53 83 399 24 1149 142 448 Cuts at: 3156 4114 442 Size: 958 1753 Fragments arranged by size: 1753 1149 958 448 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2118 2333 2354 4085 1239 Size: 879 215 21 1731 2579 Fragments arranged by size: 2579 1731 879 215 21 Bpu10I CC'TnA_GC Cuts at: 2571 2571 Size: 5425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4088 882 Size: 617 2589 2219 Fragments arranged by size: 2589 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3216 493 Size: 711 2012 2702 Fragments arranged by size: 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3232 3614 4249 275 Size: 53 83 186 936 1699 382 635 1451 Fragments arranged by size: 1699 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2580 2610 1178 Size: 30 165 30 1177 30 3993 Fragments arranged by size: 3993 1177 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2734 1186 Size: 1252 296 3877 Fragments arranged by size: 3877 1252 296 BseYI C'CCAG_C Cuts at: 2199 2278 2301 2473 4628 5124 2199 Size: 79 23 172 2155 496 2500 Fragments arranged by size: 2500 2155 496 172 79 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2310 1163 Size: 573 574 4278 Fragments arranged by size: 4278 574 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2343 2875 3636 4664 1540 Size: 275 186 342 532 761 1028 2301 Fragments arranged by size: 2301 1028 761 532 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2572 3486 3571 4642 2572 Size: 914 85 1071 3355 Fragments arranged by size: 3355 1071 914 85 BsmI GAATG_Cn' Cuts at: 2228 2936 3029 2228 Size: 708 93 4624 Fragments arranged by size: 4624 708 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2572 2744 3486 3571 4642 1391 Size: 399 443 339 172 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 339 172 85 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 2218 3314 5044 2218 Size: 1096 1730 2599 Fragments arranged by size: 2599 1730 1096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2102 2262 2780 844 Size: 1258 160 518 3489 Fragments arranged by size: 3489 1258 518 160 BsrBI CCG'CTC Cuts at: 1394 1823 2671 3312 1394 Size: 429 848 641 3507 Fragments arranged by size: 3507 848 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2490 3920 4094 65 Size: 1183 1242 1430 174 1396 Fragments arranged by size: 1430 1396 1242 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 4069 4256 1700 Size: 110 528 1731 187 2869 Fragments arranged by size: 2869 1731 528 187 110 BsrGI T'GTAC_A Cuts at: 96 2482 96 Size: 2386 3039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5425 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5425 BstYI r'GATC_y Cuts at: 1972 2205 2755 3122 3507 3524 4976 5062 5074 Size: 233 550 367 385 17 1452 86 12 Cuts at: 5074 5420 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 550 385 367 346 233 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2534 2664 2534 Size: 130 5295 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4873 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2327 2689 2941 3735 3755 2327 Size: 362 252 794 20 3997 Fragments arranged by size: 3997 794 362 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2872 3761 Size: 54 1425 50 275 18 192 850 889 Cuts at: 3761 5390 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 850 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3355 1353 Size: 2002 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4012 4386 4532 387 Size: 1064 138 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2570 2570 Size: 5425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2333 2354 3488 4085 4872 5140 Size: 393 486 215 21 1134 597 787 268 Cuts at: 5140 1239 Size: 1524 Fragments arranged by size: 1524 1134 787 597 486 393 268 215 21 EcoO109I rG'GnC_Cy Cuts at: 2160 2229 2428 2676 2160 Size: 69 199 248 4909 Fragments arranged by size: 4909 248 199 69 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 2072 4572 1678 Size: 394 2500 2531 Fragments arranged by size: 2531 2500 394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2197 2229 2630 Size: 32 135 32 461 32 132 32 401 Cuts at: 2630 2662 4066 4098 4140 4172 1373 Size: 32 1404 32 42 32 2626 Fragments arranged by size: 2626 1404 461 401 135 132 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2750 2750 Size: 5425 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2332 4539 4723 1305 Size: 1027 2207 184 2007 Fragments arranged by size: 2207 2007 1027 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 10 Size: 54 1425 343 192 3411 Fragments arranged by size: 3411 1425 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2217 2418 2559 3383 3742 3901 Size: 648 843 208 201 141 824 359 159 Cuts at: 3901 518 Size: 2042 Fragments arranged by size: 2042 843 824 648 359 208 201 159 141 MspA1I CmG'CkG Cuts at: 1606 2376 2418 2841 3518 4666 4911 1606 Size: 770 42 423 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 423 245 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4873 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2783 4328 1132 Size: 97 1554 1545 2229 Fragments arranged by size: 2229 1554 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 1108 Size: 1283 4142 PfoI T'CCnGG_A Cuts at: 2714 2714 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2160 2160 Size: 5425 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PspOMI G'GGCC_C Cuts at: 2229 2229 Size: 5425 PstI C_TGCA'G Cuts at: 830 2520 2777 830 Size: 1690 257 3478 Fragments arranged by size: 3478 1690 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 SacI G_AGCT'C Cuts at: 2572 2572 Size: 5425 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SanDI GG'GwC_CC Cuts at: 2160 2160 Size: 5425 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2516 2773 3908 4589 4780 826 Size: 219 1471 257 1135 681 191 1471 Fragments arranged by size: 1471 1471 1135 681 257 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2057 2348 2752 1872 Size: 185 291 404 4545 Fragments arranged by size: 4545 404 291 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 3529 4430 4692 820 Size: 197 926 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 2269 1517 Size: 752 4673 StyI C'CwwG_G Cuts at: 513 1065 2233 2682 2864 513 Size: 552 1168 449 182 3074 Fragments arranged by size: 3074 1168 552 449 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 2315 3452 3469 3622 3807 4252 Size: 1255 724 11 1137 17 153 185 445 Cuts at: 4252 325 Size: 1498 Fragments arranged by size: 1498 1255 1137 724 445 185 153 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2482 2598 3671 Size: 274 80 33 51 1443 505 116 1073 Cuts at: 3671 5383 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1073 505 274 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 5385 5417 2580 Size: 32 2773 32 2588 Fragments arranged by size: 2773 2588 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2055 2346 2750 1870 Size: 185 291 404 4545 Fragments arranged by size: 4545 404 291 185 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI