Restriction map for
pFN21AB7769
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbvCI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII EarI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 995 ACACGT A^C[GA][CT]GT
AgeI 647 ACCGGT A^CCGGT
ApaI 852 GGGCCC GGGCC^C
AspHI 370 GTGCTC G[AT]GC[AT]^C
BbrPI 996 CACGTG CAC^GTG
BbsI 387 GAAGAC GAAGAC(2/6)
BcgI 88 CGAGGGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1247 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 483 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 483 GCTCAGC GC^T[ACGT]AGC
BpuAI 387 GAAGAC GAAGAC(2/6)
BsaAI 996 CACGTG [CT]AC^GT[GA]
BsiEI 1336 CGGCCG CG[GA][CT]^CG
BsiHKAI 370 GTGCTC G[AT]GC[AT]^C
BsmBI 955 GAGACG CGTCTC(1/5)
BssSI 1062 CACGAG CACGAG(-5/-1)
BstAPI 363 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 713 GGTTACC G^GT[ACGT]ACC
BstPI 713 GGTTACC G^GT[ACGT]ACC
BstYI 242 AGATCC [GA]^GATC[CT]
CelII 483 GCTCAGC GC^T[ACGT]AGC
DrdI 426 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1336 CGGCCG C^GGCCG
EclXI 1336 CGGCCG C^GGCCG
Eco52I 1336 CGGCCG C^GGCCG
EcoO65I 713 GGTTACC G^GT[ACGT]ACC
KspI 1014 CCGCGG CCGC^GG
MaeII 997 ACGT A^CGT
MboII 387 GAAGA GAAGA(8/7)
MflI 242 AGATCC [GA]^GATC[CT]
MslI 1060 CACACGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 576 CCATGG C^CATGG
NheI 1092 GCTAGC G^CTAGC
PflMI 102 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 647 ACCGGT A^CCGGT
PleI 433 GAGTC GAGTC(4/5)
PmaCI 996 CACGTG CAC^GTG
PmlI 996 CACGTG CAC^GTG
PshAI 774 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1014 CCGCGG CCGC^GG
SmaI 1086 CCCGGG CCC^GGG
SmlI 55 CTTGAG C^T[CT][GA]AG
StuI 248 AGGCCT AGG^CCT
TfiI 621 GATTC G^A[AT]TC
Van91I 102 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 242 AGATCC [GA]^GATC[CT]
XmaCI 1086 CCCGGG C^CCGGG
XmaI 1086 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 778 GGCGTC G[GA]^CG[CT]C
1262 GGCGTC
AfaI 666 GTAC GT^AC
690 GTAC
AhdI 1024 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACTGGAAGTC
AlwI 243 GATCC GGATC(4/5)
341 GGATC
AspEI 1024 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACTGGAAGTC
BanI 531 GGCACC G^G[CT][GA]CC
572 GGCACC
BfaI 298 CTAG C^TAG
1093 CTAG
BsaHI 778 GGCGTC G[GA]^CG[CT]C
1262 GGCGTC
BsaI 46 GAGACC GGTCTC(1/5)
702 GGTCTC
BsaWI 647 ACCGGT [AT]^CCGG[AT]
1052 ACCGGA
BsgI 1078 GTGCAG GTGCAG(16/14)
1203 GTGCAG
BspMI 948 ACCTGC ACCTGC(4/8)
112 GCAGGT
DpnII 243 GATC ^GATC
342 GATC
DsaI 576 CCATGG C^C[GA][CT]GG
1014 CCGCGG
Eam1105I 1024 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACTGGAAGTC
EcoNI 357 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
439 CCTGGGGAAGG
EcoT14I 182 CCAAGG C^C[AT][AT]GG
576 CCATGG
FauI 74 CCCGC CCCGC(4/6)
1199 GCGGG
Hin1I 778 GGCGTC G[GA]^CG[CT]C
1262 GGCGTC
HinfI 433 GAGTC G^A[ACGT]TC
621 GATTC
MaeI 298 CTAG C^TAG
1093 CTAG
MboI 243 GATC ^GATC
342 GATC
NaeI 861 GCCGGC GCC^GGC
969 GCCGGC
NdeII 243 GATC ^GATC
342 GATC
NgoMIV 861 GCCGGC G^CCGGC
969 GCCGGC
RsaI 666 GTAC GT^AC
690 GTAC
Sau3AI 243 GATC ^GATC
342 GATC
StyI 182 CCAAGG C^C[AT][AT]GG
576 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AlwNI AocI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI Eco81I
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PpuMI PstI PvuII Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I