(Circular) (Six-base) MAPSORT of: pFN21AB7769.seq Check: 5048 from: 1 to: 6055 pFN21AB7769 6055 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2962 2962 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 3199 4118 5502 5770 1632 Size: 752 815 919 1384 268 1917 Fragments arranged by size: 1917 1384 919 815 752 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 3000 4954 5931 1203 Size: 1797 1954 977 1327 Fragments arranged by size: 1954 1797 1327 977 AgeI A'CCGG_T Cuts at: 2652 4886 2652 Size: 2234 3821 AhdI GACnn_n'nnGTC Cuts at: 3034 3307 4784 3034 Size: 273 1477 4305 Fragments arranged by size: 4305 1477 273 AleI CACnn'nnGTG Cuts at: 1166 3069 1166 Size: 1903 4152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2813 1184 Size: 1629 4426 AlwNI CAG_nnn'CTG Cuts at: 2167 2200 2227 3285 3798 5370 2167 Size: 33 27 1058 513 1572 2852 Fragments arranged by size: 2852 1572 1058 513 33 27 ApaI G_GGCC'C Cuts at: 2861 2861 Size: 6055 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 3364 3590 3742 5851 1493 Size: 1871 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2124 2243 2803 3091 3380 1870 Size: 73 181 119 560 288 289 4545 Fragments arranged by size: 4545 560 289 288 181 119 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2536 2577 3376 4831 618 Size: 325 217 627 749 41 799 1455 1842 Fragments arranged by size: 1842 1455 799 749 627 325 217 41 BanII G_rGCy'C Cuts at: 1391 2417 2514 2861 3374 1391 Size: 1026 97 347 513 4072 Fragments arranged by size: 4072 1026 513 347 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2399 928 Size: 971 500 4584 Fragments arranged by size: 4584 971 500 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2082 2116 4246 4280 1903 Size: 34 50 34 61 34 2130 34 3678 Fragments arranged by size: 3678 2130 61 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3258 4666 136 Size: 107 122 71 631 605 1586 1408 1525 Fragments arranged by size: 1586 1525 1408 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 2489 3472 2489 Size: 983 5072 Bme1580I G_kGCm'C Cuts at: 1790 2234 2580 2861 2940 4116 5272 1790 Size: 444 346 281 79 1176 1156 2573 Fragments arranged by size: 2573 1176 1156 444 346 281 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2683 2743 3786 Size: 416 53 83 399 24 1266 60 1043 Cuts at: 3786 4744 442 Size: 958 1753 Fragments arranged by size: 1753 1266 1043 958 416 399 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 3101 1056 Size: 2045 4010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2200 2200 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2237 2527 2708 2990 4715 1239 Size: 983 15 290 181 282 1725 2579 Fragments arranged by size: 2579 1725 983 290 282 181 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 4180 5045 5343 2081 Size: 2099 865 298 2793 Fragments arranged by size: 2793 2099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 2713 4718 882 Size: 617 546 668 2005 2219 Fragments arranged by size: 2219 2005 668 617 546 BsaAI yAC'GTr Cuts at: 493 1204 3003 3846 493 Size: 711 1799 843 2702 Fragments arranged by size: 2702 1799 843 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2784 3268 3862 4244 Size: 53 83 186 936 1251 484 594 382 Cuts at: 4244 4879 275 Size: 635 1451 Fragments arranged by size: 1451 1251 936 635 594 484 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2652 3057 4488 4886 5160 5307 5718 2652 Size: 405 1431 398 274 147 411 2989 Fragments arranged by size: 2989 1431 411 405 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2224 2254 2870 2900 3191 Size: 30 165 30 821 30 616 30 291 Cuts at: 3191 3221 1178 Size: 30 4012 Fragments arranged by size: 4012 821 616 291 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2303 2522 2648 3212 1186 Size: 892 225 219 126 564 4029 Fragments arranged by size: 4029 892 564 225 219 126 BseYI C'CCAG_C Cuts at: 2758 2844 5258 5754 2758 Size: 86 2414 496 3059 Fragments arranged by size: 3059 2414 496 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3104 3229 1163 Size: 573 1368 125 3989 Fragments arranged by size: 3989 1368 573 125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3344 3505 4266 5294 1540 Size: 275 186 1343 161 761 1028 2301 Fragments arranged by size: 2301 1343 1028 761 275 186 161 BsiHKAI G_wGCw'C Cuts at: 2379 4116 4201 5272 2379 Size: 1737 85 1071 3162 Fragments arranged by size: 3162 1737 1071 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 BsmBI CGTCTCn'nnnn_ Cuts at: 2954 2954 Size: 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2379 2417 2514 2580 2861 2940 Size: 399 444 145 38 97 66 281 79 Cuts at: 2940 3374 4116 4201 5272 1391 Size: 434 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 434 399 281 145 97 85 79 66 38 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 2962 3410 844 Size: 1264 854 448 3489 Fragments arranged by size: 3489 1264 854 448 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2652 2866 2974 3339 4699 4886 1700 Size: 110 842 214 108 365 1360 187 2869 Fragments arranged by size: 2869 1360 842 365 214 187 110 108 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 3067 4115 5127 3067 Size: 1048 1012 3995 Fragments arranged by size: 3995 1048 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2374 794 Size: 1580 4475 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 2718 1184 Size: 1534 4521 BstYI r'GATC_y Cuts at: 1972 2247 3385 3752 4137 4154 5606 5692 5704 Size: 275 1138 367 385 17 1452 86 12 Cuts at: 5704 6050 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 275 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2175 2286 2425 2571 2175 Size: 111 139 146 5659 Fragments arranged by size: 5659 146 139 111 BtgI C'CryG_G Cuts at: 513 1065 2581 3019 513 Size: 552 1516 438 3549 Fragments arranged by size: 3549 1516 552 438 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2696 487 Size: 683 56 1470 3846 Fragments arranged by size: 3846 1470 683 56 BtsI GCAGTG_nn' Cuts at: 2234 2503 3017 3286 3571 4365 4385 2234 Size: 269 514 269 285 794 20 3904 Fragments arranged by size: 3904 794 514 285 269 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2437 5062 809 Size: 507 1121 2625 1802 Fragments arranged by size: 2625 1802 1121 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2273 3016 3289 Size: 54 1425 50 275 18 443 743 273 Cuts at: 3289 3341 3502 4391 6020 8 Size: 52 161 889 1629 43 Fragments arranged by size: 1629 1425 889 743 443 275 273 161 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3341 3502 1537 Size: 275 1529 161 4090 Fragments arranged by size: 4090 1529 275 161 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2237 2384 2527 2708 2990 3199 Size: 393 590 15 147 143 181 282 209 Cuts at: 3199 4118 4715 5502 5770 1239 Size: 919 597 787 268 1524 Fragments arranged by size: 1524 919 787 597 590 393 282 268 209 181 147 143 15 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2448 2366 Size: 82 5973 EcoO109I rG'GnC_Cy Cuts at: 2021 2120 2453 2857 3176 2021 Size: 99 333 404 319 4900 Fragments arranged by size: 4900 404 333 319 99 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 5202 1678 Size: 3524 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2806 2838 2870 2902 3020 Size: 32 135 32 1234 32 32 32 118 Cuts at: 3020 3052 3153 3185 3293 3325 4696 4728 4770 Size: 32 101 32 108 32 1371 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1371 1234 135 118 108 101 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4233 Fragments arranged by size: 4233 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3069 4013 4372 4531 518 Size: 648 1903 944 359 159 2042 Fragments arranged by size: 2042 1903 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2297 2493 3021 3219 3232 3265 3316 Size: 666 25 196 528 198 13 33 51 Cuts at: 3316 3471 4148 5296 5541 1606 Size: 155 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 528 245 198 196 155 51 33 25 13 NaeI GCC'GGC Cuts at: 2868 2976 2868 Size: 108 5947 NcoI C'CATG_G Cuts at: 513 1065 2581 513 Size: 552 1516 3987 Fragments arranged by size: 3987 1516 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2866 2974 2866 Size: 108 5947 NheI G'CTAG_C Cuts at: 1052 3097 1052 Size: 2045 4010 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 2327 2444 2713 2854 2861 2921 1348 Size: 979 117 269 141 7 60 4482 Fragments arranged by size: 4482 979 269 141 117 60 7 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 1108 Size: 1005 5050 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PmlI CAC'GTG Cuts at: 3003 3003 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2021 2120 2453 2021 Size: 99 333 5623 Fragments arranged by size: 5623 333 99 PshAI GACnn'nnGTC Cuts at: 1613 2783 1613 Size: 1170 4885 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2857 2857 Size: 6055 PstI C_TGCA'G Cuts at: 830 2172 2319 2386 2498 2792 2959 3281 3407 Size: 1342 147 67 112 294 167 322 126 Cuts at: 3407 830 Size: 3478 Fragments arranged by size: 3478 1342 322 294 167 147 126 112 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2493 3219 3232 3316 2493 Size: 726 13 84 5232 Fragments arranged by size: 5232 726 84 13 SacII CC_GC'GG Cuts at: 3022 3022 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SanDI GG'GwC_CC Cuts at: 2021 2021 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2168 2315 2382 2494 2788 2955 3277 Size: 219 1123 147 67 112 294 167 322 Cuts at: 3277 3403 4538 5219 5410 826 Size: 126 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 681 322 294 219 191 167 147 126 112 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3093 3382 1872 Size: 1221 289 4545 Fragments arranged by size: 4545 1221 289 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 4159 5060 5322 820 Size: 197 926 117 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 117 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2255 1517 Size: 738 5317 StyI C'CwwG_G Cuts at: 513 1065 2187 2581 3494 513 Size: 552 1122 394 913 3074 Fragments arranged by size: 3074 1122 913 552 394 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2248 2631 2635 4082 4099 4252 4437 Size: 1255 668 383 4 1447 17 153 185 Cuts at: 4437 4882 325 Size: 445 1498 Fragments arranged by size: 1498 1447 1255 668 445 383 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 4301 6013 Size: 274 80 33 51 1443 693 1631 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1631 1443 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2224 2256 2517 2549 3191 3223 6015 6047 2224 Size: 32 261 32 642 32 2792 32 2232 Fragments arranged by size: 2792 2232 642 261 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3091 3380 1870 Size: 1221 289 4545 Fragments arranged by size: 4545 1221 289 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI