Restriction map for
pFN21AB7769
(Linear) (Six-base) MAPSORT of: KIBB7769.seq Check: 2321 from: 1 to: 1350
KIBB7769.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 957 1350
Size: 957 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1194 1350
Size: 379 815 156
Fragments arranged by size:
815 379 156
AflIII A'CryG_T
Cuts at: 0 995 1350
Size: 995 355
AgeI A'CCGG_T
Cuts at: 0 647 1350
Size: 647 703
AhdI GACnn_n'nnGTC
Cuts at: 0 1029 1302 1350
Size: 1029 273 48
Fragments arranged by size:
1029 273 48
AleI CACnn'nnGTG
Cuts at: 0 1064 1350
Size: 1064 286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 808 1350
Size: 808 542
AlwNI CAG_nnn'CTG
Cuts at: 0 162 195 222 1280 1350
Size: 162 33 27 1058 70
Fragments arranged by size:
1058 162 70 33 27
ApaI G_GGCC'C
Cuts at: 0 856 1350
Size: 856 494
AvaI C'yCGr_G
Cuts at: 0 119 238 798 1086 1350
Size: 119 119 560 288 264
Fragments arranged by size:
560 288 264 119 119
BanI G'GyrC_C
Cuts at: 0 531 572 1350
Size: 531 41 778
Fragments arranged by size:
778 531 41
BanII G_rGCy'C
Cuts at: 0 412 509 856 1350
Size: 412 97 347 494
Fragments arranged by size:
494 412 347 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 394 1350
Size: 394 956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 77 111 1350
Size: 77 34 1239
Fragments arranged by size:
1239 77 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1253 1350
Size: 1253 97
BlpI GC'TnA_GC
Cuts at: 0 484 1350
Size: 484 866
Bme1580I G_kGCm'C
Cuts at: 0 229 575 856 935 1350
Size: 229 346 281 79 415
Fragments arranged by size:
415 346 281 229 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 738 1350
Size: 678 60 612
Fragments arranged by size:
678 612 60
BmtI G_CTAG'C
Cuts at: 0 1096 1350
Size: 1096 254
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 195 1350
Size: 195 1155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 232 522 703 985 1350
Size: 217 15 290 181 282 365
Fragments arranged by size:
365 290 282 217 181 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1350
Size: 76 1274
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 708 1350
Size: 40 668 642
Fragments arranged by size:
668 642 40
BsaAI yAC'GTr
Cuts at: 0 998 1350
Size: 998 352
BsaHI Gr'CG_yC
Cuts at: 0 779 1263 1350
Size: 779 484 87
Fragments arranged by size:
779 484 87
BsaWI w'CCGG_w
Cuts at: 0 647 1052 1350
Size: 647 405 298
Fragments arranged by size:
647 405 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 219 249 865 895 1186 1216 1350
Size: 219 30 616 30 291 30 134
Fragments arranged by size:
616 291 219 134 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 298 517 643 1207 1350
Size: 73 225 219 126 564 143
Fragments arranged by size:
564 225 219 143 126 73
BseYI C'CCAG_C
Cuts at: 0 753 839 1350
Size: 753 86 511
Fragments arranged by size:
753 511 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1224 1350
Size: 1099 125 126
Fragments arranged by size:
1099 126 125
BsiEI CG_ry'CG
Cuts at: 0 1339 1350
Size: 1339 11
BsiHKAI G_wGCw'C
Cuts at: 0 374 1350
Size: 374 976
BsmBI CGTCTCn'nnnn_
Cuts at: 0 949 1350
Size: 949 401
Bsp1286I G_dGCh'C
Cuts at: 0 229 374 412 509 575 856 935 1350
Size: 229 145 38 97 66 281 79 415
Fragments arranged by size:
415 281 229 145 97 79 66 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 957 1350
Size: 103 854 393
Fragments arranged by size:
854 393 103
BsrFI r'CCGG_y
Cuts at: 0 647 861 969 1334 1350
Size: 647 214 108 365 16
Fragments arranged by size:
647 365 214 108 16
BssSI C'ACGA_G
Cuts at: 0 1062 1350
Size: 1062 288
BstAPI GCAn_nnn'nTGC
Cuts at: 0 369 1350
Size: 369 981
BstEII G'GTnAC_C
Cuts at: 0 713 1350
Size: 713 637
BstYI r'GATC_y
Cuts at: 0 242 1350
Size: 242 1108
Bsu36I CC'TnA_GG
Cuts at: 0 170 281 420 566 1350
Size: 170 111 139 146 784
Fragments arranged by size:
784 170 146 139 111
BtgI C'CryG_G
Cuts at: 0 576 1014 1350
Size: 576 438 336
Fragments arranged by size:
576 438 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 691 1350
Size: 691 659
BtsI GCAGTG_nn'
Cuts at: 0 229 498 1012 1281 1350
Size: 229 269 514 269 69
Fragments arranged by size:
514 269 269 229 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 1350
Size: 432 918
EaeI y'GGCC_r
Cuts at: 0 268 1011 1284 1336 1350
Size: 268 743 273 52 14
Fragments arranged by size:
743 273 268 52 14
EagI C'GGCC_G
Cuts at: 0 1336 1350
Size: 1336 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 232 379 522 703 985 1194 1350
Size: 217 15 147 143 181 282 209 156
Fragments arranged by size:
282 217 209 181 156 147 143 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 443 1350
Size: 361 82 907
Fragments arranged by size:
907 361 82
EcoO109I rG'GnC_Cy
Cuts at: 0 16 115 448 852 1171 1350
Size: 16 99 333 404 319 179
Fragments arranged by size:
404 333 319 179 99 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 801 833 865 897 1015 1047 1148 1180
Size: 801 32 32 32 118 32 101 32
Cuts at: 1180 1288 1320 1350
Size: 108 32 30
Fragments arranged by size:
801 118 108 101 32 32 32 32
32 32 30
MslI CAynn'nnrTG
Cuts at: 0 1064 1350
Size: 1064 286
MspA1I CmG'CkG
Cuts at: 0 267 292 488 1016 1214 1227 1260 1311
Size: 267 25 196 528 198 13 33 51
Cuts at: 1311 1350
Size: 39
Fragments arranged by size:
528 267 198 196 51 39 33 25
13
NaeI GCC'GGC
Cuts at: 0 863 971 1350
Size: 863 108 379
Fragments arranged by size:
863 379 108
NcoI C'CATG_G
Cuts at: 0 576 1350
Size: 576 774
NgoMIV G'CCGG_C
Cuts at: 0 861 969 1350
Size: 861 108 381
Fragments arranged by size:
861 381 108
NheI G'CTAG_C
Cuts at: 0 1092 1350
Size: 1092 258
PasI CC'CwG_GG
Cuts at: 0 322 439 708 849 856 916 1350
Size: 322 117 269 141 7 60 434
Fragments arranged by size:
434 322 269 141 117 60 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1350
Size: 108 1242
PmlI CAC'GTG
Cuts at: 0 998 1350
Size: 998 352
PpuMI rG'GwC_Cy
Cuts at: 0 16 115 448 1350
Size: 16 99 333 902
Fragments arranged by size:
902 333 99 16
PshAI GACnn'nnGTC
Cuts at: 0 778 1350
Size: 778 572
PspOMI G'GGCC_C
Cuts at: 0 852 1350
Size: 852 498
PstI C_TGCA'G
Cuts at: 0 167 314 381 493 787 954 1276 1350
Size: 167 147 67 112 294 167 322 74
Fragments arranged by size:
322 294 167 167 147 112 74 67
PvuII CAG'CTG
Cuts at: 0 488 1214 1227 1311 1350
Size: 488 726 13 84 39
Fragments arranged by size:
726 488 84 39 13
SacII CC_GC'GG
Cuts at: 0 1017 1350
Size: 1017 333
SanDI GG'GwC_CC
Cuts at: 0 16 1350
Size: 16 1334
SfcI C'TryA_G
Cuts at: 0 163 310 377 489 783 950 1272 1350
Size: 163 147 67 112 294 167 322 78
Fragments arranged by size:
322 294 167 163 147 112 78 67
SmaI CCC'GGG
Cuts at: 0 1088 1350
Size: 1088 262
SmlI C'TyrA_G
Cuts at: 0 55 1350
Size: 55 1295
StuI AGG'CCT
Cuts at: 0 250 1350
Size: 250 1100
StyI C'CwwG_G
Cuts at: 0 182 576 1350
Size: 182 394 774
Fragments arranged by size:
774 394 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 243 626 630 1350
Size: 243 383 4 720
Fragments arranged by size:
720 383 243 4
TatI w'GTAC_w
Cuts at: 0 665 1350
Size: 665 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 512 544 1186 1218 1350
Size: 219 32 261 32 642 32 132
Fragments arranged by size:
642 261 219 132 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1086 1350
Size: 1086 264
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AleI AlfI AlwNI
ApaI AvaI BanI BanII BbsI BcgI BglI BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI Eco57MI
EcoNI EcoO109I Hin4I MslI MspA1I NaeI NcoI NgoMIV
NheI PasI PflMI PmlI PpuMI PshAI PspOMI PstI
PvuII SacII SanDI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglII BmgBI Bpu10I BsaBI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstXI BstZ17I ClaI CspCI DraI DraIII EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI