(Circular) (Six-base) MAPSORT of: pFN21AB7826.seq Check: 9489 from: 1 to: 6517 pFN21AB7826 6517 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3860 1937 Size: 1923 4594 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 2748 4644 5017 1563 Size: 1185 1896 373 3063 Fragments arranged by size: 3063 1896 1185 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2422 2905 3325 4580 5964 6232 1632 Size: 790 483 420 1255 1384 268 1917 Fragments arranged by size: 1917 1384 1255 790 483 420 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 2822 5416 6393 1203 Size: 1619 2594 977 1327 Fragments arranged by size: 2594 1619 1327 977 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 5246 5246 Size: 6517 AleI CACnn'nnGTG Cuts at: 1166 3300 1166 Size: 2134 4383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6517 AlwNI CAG_nnn'CTG Cuts at: 2474 4260 5832 2474 Size: 1786 1572 3159 Fragments arranged by size: 3159 1786 1572 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2871 3291 3543 3627 3795 3826 4052 4204 Size: 1378 420 252 84 168 31 226 152 Cuts at: 4204 6313 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1378 420 252 226 168 152 84 31 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 3842 1870 Size: 73 1899 4545 Fragments arranged by size: 4545 1899 73 BamHI G'GATC_C Cuts at: 3847 4214 3847 Size: 367 6150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3838 5293 618 Size: 325 217 627 2051 1455 1842 Fragments arranged by size: 2051 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2685 3211 3836 1391 Size: 1294 526 625 4072 Fragments arranged by size: 4072 1294 625 526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3356 928 Size: 971 1457 4089 Fragments arranged by size: 4089 1457 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4708 4742 1903 Size: 34 2771 34 3678 Fragments arranged by size: 3678 2771 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2160 4409 5619 2160 Size: 2249 1210 3058 Fragments arranged by size: 3058 2249 1210 BclI T'GATC_A Cuts at: 1548 2641 1548 Size: 1093 5424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6512 6512 Size: 6517 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 4578 5734 1790 Size: 2788 1156 2573 Fragments arranged by size: 2788 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 2513 2973 Size: 416 53 83 399 24 873 223 460 Cuts at: 2973 3057 3141 3393 3729 4248 5206 442 Size: 84 84 252 336 519 958 1753 Fragments arranged by size: 1753 958 873 519 460 416 399 336 252 223 84 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 3237 3489 5177 1239 Size: 899 1099 252 1688 2579 Fragments arranged by size: 2579 1688 1099 899 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 4642 5507 5805 2717 Size: 1925 865 298 3429 Fragments arranged by size: 3429 1925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 5180 882 Size: 617 644 3037 2219 Fragments arranged by size: 3037 2219 644 617 BsaAI yAC'GTr Cuts at: 493 1204 4308 493 Size: 711 3104 2702 Fragments arranged by size: 3104 2702 711 BsaBI GATnn'nnATC Cuts at: 2247 2733 6511 2247 Size: 486 3778 2253 Fragments arranged by size: 3778 2253 486 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 4324 4706 5341 Size: 53 83 186 936 813 1978 382 635 Cuts at: 5341 275 Size: 1451 Fragments arranged by size: 1978 1451 936 813 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4950 5348 5622 5769 6180 4950 Size: 398 274 147 411 5287 Fragments arranged by size: 5287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6292 Fragments arranged by size: 6292 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 5625 BseYI C'CCAG_C Cuts at: 5720 6216 5720 Size: 496 6021 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5944 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2372 3211 3325 4578 4663 5734 2372 Size: 839 114 1253 85 1071 3155 Fragments arranged by size: 3155 1253 1071 839 114 85 BsmI GAATG_Cn' Cuts at: 3080 4028 4121 3080 Size: 948 93 5476 Fragments arranged by size: 5476 948 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 2685 3211 3325 3836 4578 4663 Size: 399 582 313 526 114 511 742 85 Cuts at: 4663 5734 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 582 526 511 399 313 114 85 BspEI T'CCGG_A Cuts at: 6180 6180 Size: 6517 BspHI T'CATG_A Cuts at: 4406 6136 4406 Size: 1730 4787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 3872 844 Size: 1590 1438 3489 Fragments arranged by size: 3489 1590 1438 BsrBI CCG'CTC Cuts at: 1394 1823 4404 1394 Size: 429 2581 3507 Fragments arranged by size: 3507 2581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5012 5186 65 Size: 1183 3764 174 1396 Fragments arranged by size: 3764 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2100 2860 2944 3784 96 Size: 2004 760 84 840 2829 Fragments arranged by size: 2829 2004 840 760 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 4577 5589 4577 Size: 1012 5505 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6517 BstBI TT'CG_AA Cuts at: 5321 5321 Size: 6517 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6517 BstYI r'GATC_y Cuts at: 1972 2232 3847 4214 4599 4616 6068 6154 6166 Size: 260 1615 367 385 17 1452 86 12 Cuts at: 6166 6512 1972 Size: 346 1977 Fragments arranged by size: 1977 1615 1452 385 367 346 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2201 513 Size: 552 1136 4829 Fragments arranged by size: 4829 1136 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5778 Fragments arranged by size: 5778 683 56 BtsI GCAGTG_nn' Cuts at: 4033 4827 4847 4033 Size: 794 20 5703 Fragments arranged by size: 5703 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 2379 2575 3821 2379 Size: 196 1246 5075 Fragments arranged by size: 5075 1246 196 DraIII CAC_nnn'GTG Cuts at: 3805 3805 Size: 6517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5524 809 Size: 507 4208 1802 Fragments arranged by size: 4208 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3964 4853 6482 Size: 54 1425 50 275 18 2134 889 1629 Cuts at: 6482 8 Size: 43 Fragments arranged by size: 2134 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2270 2441 4447 1353 Size: 837 80 171 2006 3423 Fragments arranged by size: 3423 2006 837 171 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3209 3209 Size: 6517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2422 2905 3237 3325 3489 4580 Size: 393 506 284 483 332 88 164 1091 Cuts at: 4580 5177 5964 6232 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1091 787 597 506 483 393 332 284 268 164 88 EcoNI CCTnn'n_nnAGG Cuts at: 3343 3343 Size: 6517 EcoO109I rG'GnC_Cy Cuts at: 2094 2094 Size: 6517 EcoRI G'AATT_C Cuts at: 2871 3291 3543 3627 3795 3826 4204 2871 Size: 420 252 84 168 31 378 5184 Fragments arranged by size: 5184 420 378 252 168 84 31 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 5664 1678 Size: 3986 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 5158 5190 5232 Size: 32 135 32 587 32 2967 32 42 Cuts at: 5232 5264 1373 Size: 32 2626 Fragments arranged by size: 2967 2626 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 3861 4107 669 Size: 988 281 520 1403 246 3079 Fragments arranged by size: 3079 1403 988 520 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 2458 4107 2458 Size: 1649 4868 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 4116 4116 Size: 6517 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 5631 5815 1305 Size: 858 3468 184 2007 Fragments arranged by size: 3468 2007 858 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4695 Fragments arranged by size: 4695 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2562 3300 4475 4834 4993 518 Size: 648 1396 738 1175 359 159 2042 Fragments arranged by size: 2042 1396 1175 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2404 3190 3933 4610 5758 6003 1606 Size: 798 786 743 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 786 743 677 245 NcoI C'CATG_G Cuts at: 513 1065 2201 513 Size: 552 1136 4829 Fragments arranged by size: 4829 1136 552 NdeI CA'TA_TG Cuts at: 387 2893 3653 387 Size: 2506 760 3251 Fragments arranged by size: 3251 2506 760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 2826 2910 3875 5420 1132 Size: 97 1597 84 965 1545 2229 Fragments arranged by size: 2229 1597 1545 965 97 84 PasI CC'CwG_GG Cuts at: 1348 2017 1348 Size: 669 5848 PciI A'CATG_T Cuts at: 2822 5416 2822 Size: 2594 3923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6517 PfoI T'CCnGG_A Cuts at: 3613 3613 Size: 6517 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4586 4618 6099 6131 4586 Size: 32 1481 32 4972 Fragments arranged by size: 4972 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2094 2094 Size: 6517 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 2616 4087 2616 Size: 1471 5046 PstI C_TGCA'G Cuts at: 830 2409 3869 830 Size: 1579 1460 3478 Fragments arranged by size: 3478 1579 1460 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 PvuII CAG'CTG Cuts at: 2404 3190 2404 Size: 786 5731 SacI G_AGCT'C Cuts at: 3211 3211 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SapI GCTCTTCn'nnn_ Cuts at: 2190 2190 Size: 6517 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 4765 6477 4765 Size: 1712 4805 SexAI A'CCwGG_T Cuts at: 2133 2133 Size: 6517 SfcI C'TryA_G Cuts at: 826 1045 2125 2405 3865 5000 5681 5872 826 Size: 219 1080 280 1460 1135 681 191 1471 Fragments arranged by size: 1471 1460 1135 1080 681 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 4621 5522 5784 820 Size: 197 926 753 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 753 262 197 SnaBI TAC'GTA Cuts at: 493 4308 493 Size: 3815 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6517 SphI G_CATG'C Cuts at: 1132 2910 3875 1132 Size: 1778 965 3774 Fragments arranged by size: 3774 1778 965 SspI AAT'ATT Cuts at: 5 52 2514 2596 4441 5 Size: 47 2462 82 1845 2081 Fragments arranged by size: 2462 2081 1845 82 47 StuI AGG'CCT Cuts at: 1517 3170 3674 3758 1517 Size: 1653 504 84 4276 Fragments arranged by size: 4276 1653 504 84 StyI C'CwwG_G Cuts at: 513 1065 2201 3956 513 Size: 552 1136 1755 3074 Fragments arranged by size: 3074 1755 1136 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 4544 4561 4714 4899 5344 325 Size: 1255 492 2472 17 153 185 445 1498 Fragments arranged by size: 2472 1498 1255 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2860 2944 Size: 274 80 33 51 1443 123 760 84 Cuts at: 2944 3404 3572 3784 4763 6475 96 Size: 460 168 212 979 1712 138 Fragments arranged by size: 1712 1443 979 760 460 274 212 168 138 123 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6477 6509 6477 Size: 32 6485 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6517 XbaI T'CTAG_A Cuts at: 2416 3853 2416 Size: 1437 5080 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 3086 3254 3590 3758 4646 3086 Size: 168 336 168 888 4957 Fragments arranged by size: 4957 888 336 168 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI