(Circular) (Six-base) MAPSORT of: pFN21AB9772.seq Check: 455 from: 1 to: 6352 pFN21AB9772 6352 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 2611 3695 1937 Size: 406 268 1084 4594 Fragments arranged by size: 4594 1084 406 268 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 2386 4479 4852 1563 Size: 823 2093 373 3063 Fragments arranged by size: 3063 2093 823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2147 2401 2501 3490 3574 4415 5799 Size: 474 41 254 100 989 84 841 1384 Cuts at: 5799 6067 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 989 841 474 268 254 100 84 41 AflII C'TTAA_G Cuts at: 820 1017 2282 2851 820 Size: 197 1265 569 4321 Fragments arranged by size: 4321 1265 569 197 AflIII A'CryG_T Cuts at: 1203 2290 3398 3434 5251 6228 1203 Size: 1087 1108 36 1817 977 1327 Fragments arranged by size: 1817 1327 1108 1087 977 36 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 4095 5667 4095 Size: 1572 4780 ApaLI G'TGCA_C Cuts at: 2452 4409 5565 2452 Size: 1957 1156 3239 Fragments arranged by size: 3239 1957 1156 ApoI r'AATT_y Cuts at: 1493 2023 3007 3036 3288 3661 3887 4039 6148 Size: 530 984 29 252 373 226 152 2109 Cuts at: 6148 1493 Size: 1697 Fragments arranged by size: 2109 1697 984 530 373 252 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 2502 4906 160 Size: 872 1470 2404 1606 Fragments arranged by size: 2404 1606 1470 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 2968 3456 3682 4049 2968 Size: 488 226 367 5271 Fragments arranged by size: 5271 488 367 226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3673 5128 618 Size: 325 217 627 1886 1455 1842 Fragments arranged by size: 1886 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3671 1391 Size: 2280 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4543 4577 1903 Size: 34 50 34 2522 34 3678 Fragments arranged by size: 3678 2522 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 3025 1548 Size: 1477 4875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4963 136 Size: 107 122 71 631 605 3291 1525 Fragments arranged by size: 3291 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2924 6347 2924 Size: 3423 2929 BlpI GC'TnA_GC Cuts at: 3769 3769 Size: 6352 Bme1580I G_kGCm'C Cuts at: 1790 2456 4413 5569 1790 Size: 666 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 666 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 4083 5041 Size: 416 53 83 399 24 635 2031 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 2031 1753 958 635 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2567 2206 Size: 361 5991 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2176 2248 2397 2730 2814 3234 3318 Size: 791 146 72 149 333 84 420 84 Cuts at: 3318 3334 3486 3570 5012 1239 Size: 16 152 84 1442 2579 Fragments arranged by size: 2579 1442 791 420 333 152 149 146 84 84 84 72 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4477 5342 5640 4477 Size: 865 298 5189 Fragments arranged by size: 5189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 3435 4143 493 Size: 711 2195 36 708 2702 Fragments arranged by size: 2702 2195 711 708 36 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 5183 5457 5604 6015 4785 Size: 398 274 147 411 5122 Fragments arranged by size: 5122 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2217 2247 1178 Size: 30 165 30 814 30 5283 Fragments arranged by size: 5283 814 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6352 BseYI C'CCAG_C Cuts at: 5555 6051 5555 Size: 496 5856 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2120 2342 1163 Size: 573 384 222 5173 Fragments arranged by size: 5173 573 384 222 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2456 4413 4498 5569 2456 Size: 1957 85 1071 3239 Fragments arranged by size: 3239 1957 1071 85 BsmI GAATG_Cn' Cuts at: 2900 3343 3863 3956 2900 Size: 443 520 93 5296 Fragments arranged by size: 5296 520 443 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2456 3671 4413 4498 5569 1391 Size: 399 666 1215 742 85 1071 2174 Fragments arranged by size: 2174 1215 1071 742 666 399 85 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 3616 4241 5971 3616 Size: 625 1730 3997 Fragments arranged by size: 3997 1730 625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3707 844 Size: 2863 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2255 4847 5021 65 Size: 1183 1007 2592 174 1396 Fragments arranged by size: 2592 1396 1183 1007 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6352 BstBI TT'CG_AA Cuts at: 3034 3610 5156 3034 Size: 576 1546 4230 Fragments arranged by size: 4230 1546 576 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstYI r'GATC_y Cuts at: 1972 2924 2968 3176 3456 3682 4049 4434 4451 Size: 952 44 208 280 226 367 385 17 Cuts at: 4451 5903 5989 6001 6347 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 952 385 367 346 280 226 208 86 44 17 12 BstZ17I GTA'TAC Cuts at: 2612 2612 Size: 6352 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5800 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 2485 2958 3868 4662 4682 2485 Size: 473 910 794 20 4155 Fragments arranged by size: 4155 910 794 473 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2581 2616 2887 2922 529 Size: 35 2017 35 271 35 3959 Fragments arranged by size: 3959 2017 271 35 35 35 DraI TTT'AAA Cuts at: 2949 3656 2949 Size: 707 5645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 3799 4688 Size: 54 1425 50 275 18 198 1771 889 Cuts at: 4688 6317 8 Size: 1629 43 Fragments arranged by size: 1771 1629 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2079 4282 1353 Size: 701 25 2203 3423 Fragments arranged by size: 3423 2203 701 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2106 2147 2176 2248 2397 2401 Size: 393 398 76 41 29 72 149 4 Cuts at: 2401 2501 2730 2814 3234 3318 3334 3486 3490 Size: 100 229 84 420 84 16 152 4 Cuts at: 3490 3570 3574 4415 5012 5799 6067 1239 Size: 80 4 841 597 787 268 1524 Fragments arranged by size: 1524 841 787 597 420 398 393 268 229 152 149 100 84 84 80 76 72 41 29 16 4 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2165 2752 2165 Size: 587 5765 EcoO109I rG'GnC_Cy Cuts at: 3138 3138 Size: 6352 EcoRI G'AATT_C Cuts at: 3036 3288 3661 4039 3036 Size: 252 373 378 5349 Fragments arranged by size: 5349 378 373 252 EcoRV GAT'ATC Cuts at: 2042 2042 Size: 6352 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2156 2989 2156 Size: 833 5519 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 3384 5499 1678 Size: 1706 2115 2531 Fragments arranged by size: 2531 2115 1706 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2217 2249 3629 Size: 32 135 32 459 32 154 32 1380 Cuts at: 3629 3661 4993 5025 5067 5099 1373 Size: 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1380 1332 459 154 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2765 2858 3442 3696 3942 669 Size: 988 281 827 93 584 254 246 3079 Fragments arranged by size: 3079 988 827 584 281 254 246 93 HindIII A'AGCT_T Cuts at: 748 2118 2466 748 Size: 1370 348 4634 Fragments arranged by size: 4634 1370 348 HpaI GTT'AAC Cuts at: 3442 3942 3442 Size: 500 5852 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5466 5650 1305 Size: 4161 184 2007 Fragments arranged by size: 4161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 10 Size: 54 1425 343 198 4332 Fragments arranged by size: 4332 1425 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2323 2877 3397 3401 3633 4310 4669 Size: 648 1157 554 520 4 232 677 359 Cuts at: 4669 4828 518 Size: 159 2042 Fragments arranged by size: 2042 1157 677 648 554 520 359 232 159 4 MspA1I CmG'CkG Cuts at: 1606 3768 4445 5593 5838 1606 Size: 2162 677 1148 245 2120 Fragments arranged by size: 2162 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5800 NdeI CA'TA_TG Cuts at: 387 2862 387 Size: 2475 3877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2294 3116 3370 3454 3632 3710 5255 Size: 97 1065 822 254 84 178 78 1545 Cuts at: 5255 1132 Size: 2229 Fragments arranged by size: 2229 1545 1065 822 254 178 97 84 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 2290 5251 2290 Size: 2961 3391 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PmlI CAC'GTG Cuts at: 3399 3435 3399 Size: 36 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 2808 3922 2808 Size: 1114 5238 PstI C_TGCA'G Cuts at: 830 3704 830 Size: 2874 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SexAI A'CCwGG_T Cuts at: 2753 2753 Size: 6352 SfcI C'TryA_G Cuts at: 826 1045 3700 4835 5516 5707 826 Size: 219 2655 1135 681 191 1471 Fragments arranged by size: 2655 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2851 4456 5357 5619 820 Size: 197 926 339 569 1605 901 262 1553 Fragments arranged by size: 1605 1553 926 901 569 339 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3116 3632 3710 1132 Size: 1984 516 78 3774 Fragments arranged by size: 3774 1984 516 78 SspI AAT'ATT Cuts at: 5 52 2689 4276 5 Size: 47 2637 1587 2081 Fragments arranged by size: 2637 2081 1587 47 StuI AGG'CCT Cuts at: 1517 3167 1517 Size: 1650 4702 StyI C'CwwG_G Cuts at: 513 1065 3791 513 Size: 552 2726 3074 Fragments arranged by size: 3074 2726 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2867 4379 4396 4549 4734 5179 325 Size: 1255 1287 1512 17 153 185 445 1498 Fragments arranged by size: 1512 1498 1287 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4598 6310 96 Size: 274 80 33 51 1443 2621 1712 138 Fragments arranged by size: 2621 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2554 2586 6312 6344 2554 Size: 32 3726 32 2562 Fragments arranged by size: 3726 2562 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 2495 2747 3083 3503 3587 4481 2495 Size: 252 336 420 84 894 4366 Fragments arranged by size: 4366 894 420 336 252 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI