(Circular) (Six-base) MAPSORT of: pFN21AB7791.seq Check: 8430 from: 1 to: 6082 pFN21AB7791 6082 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2588 2588 Size: 6082 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3425 1937 Size: 1488 4594 Acc65I G'GTAC_C Cuts at: 3403 3403 Size: 6082 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 3133 4145 5529 5797 1632 Size: 544 957 1012 1384 268 1917 Fragments arranged by size: 1917 1384 1012 957 544 268 AfeI AGC'GCT Cuts at: 2144 2978 2144 Size: 834 5248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 4981 5958 1203 Size: 3778 977 1327 Fragments arranged by size: 3778 1327 977 AgeI A'CCGG_T Cuts at: 3275 4913 3275 Size: 1638 4444 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 3040 3276 1166 Size: 1874 236 3972 Fragments arranged by size: 3972 1874 236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6082 AlwNI CAG_nnn'CTG Cuts at: 2257 2284 3219 3352 3825 5397 2257 Size: 27 935 133 473 1572 2942 Fragments arranged by size: 2942 1572 935 473 133 27 ApaI G_GGCC'C Cuts at: 2879 2879 Size: 6082 ApaLI G'TGCA_C Cuts at: 3101 3269 4139 5295 3101 Size: 168 870 1156 3888 Fragments arranged by size: 3888 1156 870 168 ApoI r'AATT_y Cuts at: 1493 3391 3617 3769 5878 1493 Size: 1898 226 152 2109 1697 Fragments arranged by size: 2109 1898 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2332 2429 2640 2825 3407 1870 Size: 73 288 101 97 211 185 582 4545 Fragments arranged by size: 4545 582 288 211 185 101 97 73 BamHI G'GATC_C Cuts at: 2447 3412 3779 2447 Size: 965 367 4750 Fragments arranged by size: 4750 965 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2654 2773 2782 2919 3006 Size: 325 217 627 867 119 9 137 87 Cuts at: 3006 3403 4858 618 Size: 397 1455 1842 Fragments arranged by size: 1842 1455 867 627 397 325 217 137 119 87 9 BanII G_rGCy'C Cuts at: 1391 2211 2736 2825 2879 2993 3401 1391 Size: 820 525 89 54 114 408 4072 Fragments arranged by size: 4072 820 525 408 114 89 54 BbeI G_GCGC'C Cuts at: 2923 2923 Size: 6082 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BbvCI CC'TCA_GC Cuts at: 2835 2835 Size: 6082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5184 3974 Size: 1210 4872 BclI T'GATC_A Cuts at: 1548 1548 Size: 6082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2796 3096 3167 Size: 107 122 71 631 605 1124 300 71 Cuts at: 3167 3365 4693 136 Size: 198 1328 1525 Fragments arranged by size: 1525 1328 1124 631 605 300 198 122 107 71 71 BglII A'GATC_T Cuts at: 6077 6077 Size: 6082 BlpI GC'TnA_GC Cuts at: 2670 3499 2670 Size: 829 5253 Bme1580I G_kGCm'C Cuts at: 1790 2412 2485 2657 2879 3047 3105 3273 4143 Size: 622 73 172 222 168 58 168 870 Cuts at: 4143 5299 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 870 622 222 172 168 168 73 58 BmgBI CAC'GTC Cuts at: 1318 2806 1318 Size: 1488 4594 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2873 3813 4771 Size: 416 53 83 399 24 1456 940 958 Cuts at: 4771 442 Size: 1753 Fragments arranged by size: 1753 1456 958 940 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2294 2839 4742 1239 Size: 1040 15 545 1903 2579 Fragments arranged by size: 2579 1903 1040 545 15 Bpu10I CC'TnA_GC Cuts at: 2835 2835 Size: 6082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4207 5072 5370 4207 Size: 865 298 4919 Fragments arranged by size: 4919 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4745 882 Size: 617 3246 2219 Fragments arranged by size: 3246 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3873 493 Size: 711 2669 2702 Fragments arranged by size: 2702 2669 711 BsaBI GATnn'nnATC Cuts at: 6076 6076 Size: 6082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2920 3889 4271 4906 Size: 53 83 186 936 1387 969 382 635 Cuts at: 4906 275 Size: 1451 Fragments arranged by size: 1451 1387 969 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3275 4515 4913 5187 5334 5745 3275 Size: 1240 398 274 147 411 3612 Fragments arranged by size: 3612 1240 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2449 2479 1178 Size: 30 165 30 1046 30 4781 Fragments arranged by size: 4781 1046 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 2432 2843 3064 1186 Size: 1174 72 411 221 4204 Fragments arranged by size: 4204 1174 411 221 72 BseYI C'CCAG_C Cuts at: 2103 2313 2529 2985 3369 5285 5781 2103 Size: 210 216 456 384 1916 496 2404 Fragments arranged by size: 2404 1916 496 456 384 216 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3338 1163 Size: 573 1602 3907 Fragments arranged by size: 3907 1602 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2495 2861 3532 4293 5321 1540 Size: 275 186 494 366 671 761 1028 2301 Fragments arranged by size: 2301 1028 761 671 494 366 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 3105 3273 4143 4228 5299 2211 Size: 894 168 870 85 1071 2994 Fragments arranged by size: 2994 1071 894 870 168 85 BsmI GAATG_Cn' Cuts at: 3593 3686 3593 Size: 93 5989 BsmBI CGTCTCn'nnnn_ Cuts at: 2561 2561 Size: 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2412 2485 2657 2736 2825 2879 Size: 399 421 201 73 172 79 89 54 Cuts at: 2879 2993 3047 3105 3273 3401 4143 4228 5299 Size: 114 54 58 168 128 742 85 1071 Cuts at: 5299 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 421 399 201 172 168 128 114 89 85 79 73 58 54 54 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 2182 3971 5701 2182 Size: 1789 1730 2563 Fragments arranged by size: 2563 1789 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2588 3258 3437 844 Size: 1744 670 179 3489 Fragments arranged by size: 3489 1744 670 179 BsrBI CCG'CTC Cuts at: 1394 1823 3283 3969 1394 Size: 429 1460 686 3507 Fragments arranged by size: 3507 1460 686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4577 4751 65 Size: 1183 3329 174 1396 Fragments arranged by size: 3329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2776 2856 2916 3275 4726 4913 1700 Size: 110 966 80 60 359 1451 187 2869 Fragments arranged by size: 2869 1451 966 359 187 110 80 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6082 BssHII G'CGCG_C Cuts at: 1925 2889 3325 3327 1925 Size: 964 436 2 4680 Fragments arranged by size: 4680 964 436 2 BssSI C'ACGA_G Cuts at: 4142 5154 4142 Size: 1012 5070 BstAPI GCAn_nnn'nTGC Cuts at: 794 3219 794 Size: 2425 3657 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstXI CCAn_nnnn'nTGG Cuts at: 2039 2039 Size: 6082 BstYI r'GATC_y Cuts at: 1972 2253 2304 2447 2677 3412 3779 4164 4181 Size: 281 51 143 230 735 367 385 17 Cuts at: 4181 5633 5719 5731 6077 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 735 385 367 346 281 230 143 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 2599 3375 513 Size: 552 939 453 142 776 3220 Fragments arranged by size: 3220 939 776 552 453 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3322 487 Size: 683 56 2096 3247 Fragments arranged by size: 3247 2096 683 56 BtsI GCAGTG_nn' Cuts at: 2972 3598 4392 4412 2972 Size: 626 794 20 4642 Fragments arranged by size: 4642 794 626 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6047 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2492 2858 2903 Size: 54 1425 50 275 18 662 366 45 Cuts at: 2903 3168 3529 4418 6047 8 Size: 265 361 889 1629 43 Fragments arranged by size: 1629 1425 889 662 366 361 275 265 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2492 2858 3529 1537 Size: 275 680 366 671 4090 Fragments arranged by size: 4090 680 671 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 2687 4012 1353 Size: 1194 140 1325 3423 Fragments arranged by size: 3423 1325 1194 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2438 4669 5043 5189 387 Size: 1064 138 849 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 849 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 2209 Size: 6082 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2279 2294 2839 3133 4145 4742 Size: 393 544 103 15 545 294 1012 597 Cuts at: 4742 5529 5797 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1012 787 597 545 544 393 294 268 103 15 EcoNI CCTnn'n_nnAGG Cuts at: 2762 2762 Size: 6082 EcoO109I rG'GnC_Cy Cuts at: 2035 2368 2462 2932 2035 Size: 333 94 470 5185 Fragments arranged by size: 5185 470 333 94 EcoRI G'AATT_C Cuts at: 3391 3769 3391 Size: 378 5704 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 2146 2529 2923 2980 3162 5229 1678 Size: 468 383 394 57 182 2067 2531 Fragments arranged by size: 2531 2067 468 394 383 182 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 4723 4755 4797 Size: 32 135 32 824 32 2295 32 42 Cuts at: 4797 4829 1373 Size: 32 2626 Fragments arranged by size: 2626 2295 824 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3426 3672 669 Size: 988 281 1488 246 3079 Fragments arranged by size: 3079 1488 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6082 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KasI G'GCGC_C Cuts at: 2919 2919 Size: 6082 KpnI G_GTAC'C Cuts at: 3407 3407 Size: 6082 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4260 Fragments arranged by size: 4260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 3040 3264 3276 4040 4399 4558 Size: 648 946 928 224 12 764 359 159 Cuts at: 4558 518 Size: 2042 Fragments arranged by size: 2042 946 928 764 648 359 224 159 12 MspA1I CmG'CkG Cuts at: 1606 2220 2674 2958 2985 3498 4175 5323 5568 Size: 614 454 284 27 513 677 1148 245 Cuts at: 5568 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 614 513 454 284 245 27 NaeI GCC'GGC Cuts at: 2918 2918 Size: 6082 NarI GG'CG_CC Cuts at: 2920 2920 Size: 6082 NcoI C'CATG_G Cuts at: 513 1065 2004 2599 513 Size: 552 939 595 3996 Fragments arranged by size: 3996 939 595 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6082 NgoMIV G'CCGG_C Cuts at: 2916 2916 Size: 6082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 3440 4985 1132 Size: 97 2211 1545 2229 Fragments arranged by size: 2229 2211 1545 97 PasI CC'CwG_GG Cuts at: 1348 2039 2722 2872 1348 Size: 691 683 150 4558 Fragments arranged by size: 4558 691 683 150 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 3376 1108 Size: 2268 3814 PfoI T'CCnGG_A Cuts at: 2256 2560 2708 2816 2928 2256 Size: 304 148 108 112 5410 Fragments arranged by size: 5410 304 148 112 108 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2368 2932 2368 Size: 564 5518 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PspOMI G'GGCC_C Cuts at: 2875 2875 Size: 6082 PstI C_TGCA'G Cuts at: 830 2440 2766 3215 3434 830 Size: 1610 326 449 219 3478 Fragments arranged by size: 3478 1610 449 326 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 PvuII CAG'CTG Cuts at: 2958 2958 Size: 6082 SacI G_AGCT'C Cuts at: 2211 2211 Size: 6082 SalI G'TCGA_C Cuts at: 1936 3424 1936 Size: 1488 4594 SapI GCTCTTCn'nnn_ Cuts at: 2547 2547 Size: 6082 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SexAI A'CCwGG_T Cuts at: 3081 3081 Size: 6082 SfcI C'TryA_G Cuts at: 826 1045 2372 2436 2762 3211 3430 4565 5246 Size: 219 1327 64 326 449 219 1135 681 Cuts at: 5246 5437 826 Size: 191 1471 Fragments arranged by size: 1471 1327 1135 681 449 326 219 219 191 64 SfoI GGC'GCC Cuts at: 2921 2921 Size: 6082 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 2916 3275 1700 Size: 1216 359 4507 Fragments arranged by size: 4507 1216 359 SmaI CCC'GGG Cuts at: 1872 2334 2642 3409 1872 Size: 462 308 767 4545 Fragments arranged by size: 4545 767 462 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 4186 5087 5349 820 Size: 197 926 2243 901 262 1553 Fragments arranged by size: 2243 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 3440 1132 Size: 2308 3774 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 2189 2840 1517 Size: 672 651 4759 Fragments arranged by size: 4759 672 651 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2244 2358 2599 3094 3521 Size: 552 939 12 228 114 241 495 427 Cuts at: 3521 513 Size: 3074 Fragments arranged by size: 3074 939 552 495 427 241 228 114 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 2923 4109 4126 4279 4464 4909 Size: 1255 725 618 1186 17 153 185 445 Cuts at: 4909 325 Size: 1498 Fragments arranged by size: 1498 1255 1186 725 618 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4328 6040 96 Size: 274 80 33 51 1443 2351 1712 138 Fragments arranged by size: 2351 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2449 2481 6042 6074 2449 Size: 32 3561 32 2457 Fragments arranged by size: 3561 2457 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2812 1316 Size: 1496 4586 XbaI T'CTAG_A Cuts at: 3418 3418 Size: 6082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2391 2111 Size: 280 5802 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 2332 2640 3407 1870 Size: 462 308 767 4545 Fragments arranged by size: 4545 767 462 308 XmnI GAAnn'nnTTC Cuts at: 2055 3119 3203 4211 2055 Size: 1064 84 1008 3926 Fragments arranged by size: 3926 1064 1008 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI