Restriction map for
pFN21AB7717
(Circular) (Six-base) MAPSORT of: pFN21AB7717.seq Check: 752 from: 1 to: 5980
pFN21AB7717 5980 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2295 2412 2617 4043 5427 5695 1632
Size: 663 117 205 1426 1384 268 1917
Fragments arranged by size:
1917 1426 1384 663 268 205 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 2728 4709 2728
Size: 1981 3999
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2535 3723 5295 2535
Size: 1188 1572 3220
Fragments arranged by size:
3220 1572 1188
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2375 2499 2751 2835 3289 3515 3667 5776
Size: 882 124 252 84 454 226 152 2109
Cuts at: 5776 1493
Size: 1697
Fragments arranged by size:
2109 1697 882 454 252 226 152 124
84
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2457 3305 1870
Size: 73 514 848 4545
Fragments arranged by size:
4545 848 514 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3147 3301 4756 618
Size: 325 217 627 1360 154 1455 1842
Fragments arranged by size:
1842 1455 1360 627 325 217 154
BanII G_rGCy'C
Cuts at: 1391 2687 3299 1391
Size: 1296 612 4072
Fragments arranged by size:
4072 1296 612
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2549 928
Size: 971 162 488 4359
Fragments arranged by size:
4359 971 488 162
BbvCI CC'TCA_GC
Cuts at: 2651 2651
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2042 2076 4171 4205 1903
Size: 34 105 34 2095 34 3678
Fragments arranged by size:
3678 2095 105 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2230 5975 2230
Size: 3745 2235
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2601 2685
Size: 416 53 83 399 24 873 311 84
Cuts at: 2685 3711 4669 442
Size: 1026 958 1753
Fragments arranged by size:
1753 1026 958 873 416 399 311 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2289 2287
Size: 2 5978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2781 2865 3117 4640 1239
Size: 917 625 84 252 1523 2579
Fragments arranged by size:
2579 1523 917 625 252 84
Bpu10I CC'TnA_GC
Cuts at: 2651 2651
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 1186
Size: 1075 4905
BseYI C'CCAG_C
Cuts at: 3011 5183 5679 3011
Size: 2172 496 3312
Fragments arranged by size:
3312 2172 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2716 1163
Size: 573 980 4427
Fragments arranged by size:
4427 980 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2708 4041 4126 5197 2708
Size: 1333 85 1071 3491
Fragments arranged by size:
3491 1333 1071 85
BsmI GAATG_Cn'
Cuts at: 2348 2531 3119 3128 3203 3491 3584 2348
Size: 183 588 9 75 288 93 4744
Fragments arranged by size:
4744 588 288 183 93 75 9
BsmBI CGTCTCn'nnnn_
Cuts at: 2988 2988
Size: 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 2708 3299 4041 4126 5197 1391
Size: 399 897 21 591 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 591 399 85 21
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3063 3867 1394
Size: 429 1240 804 3507
Fragments arranged by size:
3507 1240 804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3150 4624 4811 1700
Size: 110 1340 1474 187 2869
Fragments arranged by size:
2869 1474 1340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2703 3123 794
Size: 1909 420 3651
Fragments arranged by size:
3651 1909 420
BstBI TT'CG_AA
Cuts at: 2052 4784 2052
Size: 2732 3248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2230 3087 3310 3677 4062 4079 5531 5617
Size: 258 857 223 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 857 385 367 346 258 223
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2219 513
Size: 552 939 60 155 4274
Fragments arranged by size:
4274 939 552 155 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2704 3496 4290 4310 2536
Size: 168 792 794 20 4206
Fragments arranged by size:
4206 794 792 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2770 2805 529
Size: 35 2206 35 3704
Fragments arranged by size:
3704 2206 35 35
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 3207 3207
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2258 3427 4316
Size: 54 1425 50 275 18 428 1169 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1169 889 428 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2198 3910 1353
Size: 723 122 1712 3423
Fragments arranged by size:
3423 1712 723 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2295 2412 2617 2781 2865 3117
Size: 393 524 139 117 205 164 84 252
Cuts at: 3117 4043 4640 5427 5695 1239
Size: 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 597 524 393 268 252
205 164 139 117 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 5980
EcoO109I rG'GnC_Cy
Cuts at: 2113 3016 2113
Size: 903 5077
EcoRI G'AATT_C
Cuts at: 2751 2835 3289 3667 2751
Size: 84 454 378 5064
Fragments arranged by size:
5064 454 378 84
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2215 2247 2306 2338 2546
Size: 32 135 32 643 32 59 32 208
Cuts at: 2546 2578 2714 2746 2798 2830 3050 3082 4621
Size: 32 136 32 52 32 220 32 1539
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1539 643 220 208 136 135 59
52 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2964 3048 748
Size: 2216 84 3680
Fragments arranged by size:
3680 2216 84
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 2198 3579 2198
Size: 1381 4599
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2045 5094 5278 1305
Size: 740 3049 184 2007
Fragments arranged by size:
3049 2007 740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2260 10
Size: 54 1425 343 428 3730
Fragments arranged by size:
3730 1425 428 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3112 3168 3938 4297 4456 518
Size: 648 1705 241 56 770 359 159 2042
Fragments arranged by size:
2042 1705 770 648 359 241 159 56
MspA1I CmG'CkG
Cuts at: 1606 3145 3396 4073 5221 5466 1606
Size: 1539 251 677 1148 245 2120
Fragments arranged by size:
2120 1539 1148 677 251 245
NaeI GCC'GGC
Cuts at: 3152 3152
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 3109 387
Size: 2722 3258
NgoMIV G'CCGG_C
Cuts at: 3150 3150
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 3016 2113
Size: 903 5077
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SanDI GG'GwC_CC
Cuts at: 3016 3016
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 3328 4463 5144 5335 826
Size: 219 2283 1135 681 191 1471
Fragments arranged by size:
2283 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2459 3307 1872
Size: 587 848 4545
Fragments arranged by size:
4545 848 587
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4084 4985 5247 820
Size: 197 926 2141 901 262 1553
Fragments arranged by size:
2141 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2347 3419 513
Size: 552 939 343 1072 3074
Fragments arranged by size:
3074 1072 939 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2407 4007 4024 4177 4362 4807 325
Size: 1255 827 1600 17 153 185 445 1498
Fragments arranged by size:
1600 1498 1255 827 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 5940 5972 2204
Size: 32 3704 32 2212
Fragments arranged by size:
3704 2212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 2275 3316 2275
Size: 1041 4939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2401
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 2457 3305 1870
Size: 587 848 4545
Fragments arranged by size:
4545 848 587
XmnI GAAnn'nnTTC
Cuts at: 2630 2966 3218 4109 2630
Size: 336 252 891 4501
Fragments arranged by size:
4501 891 336 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI