(Circular) (Six-base) MAPSORT of: pFN21AB7853.seq Check: 8968 from: 1 to: 6352 pFN21AB7853 6352 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2867 3239 2867 Size: 372 5980 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 4415 5799 6067 1632 Size: 1302 1481 1384 268 1917 Fragments arranged by size: 1917 1481 1384 1302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 5251 6228 1203 Size: 4048 977 1327 Fragments arranged by size: 4048 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2054 2086 2054 Size: 32 6320 AlwNI CAG_nnn'CTG Cuts at: 2087 2272 2380 2525 4095 5667 2087 Size: 185 108 145 1570 1572 2772 Fragments arranged by size: 2772 1572 1570 185 145 108 ApaLI G'TGCA_C Cuts at: 3316 4409 5565 3316 Size: 1093 1156 4103 Fragments arranged by size: 4103 1156 1093 ApoI r'AATT_y Cuts at: 1493 2509 2798 2899 3262 3661 3887 4039 6148 Size: 1016 289 101 363 399 226 152 2109 Cuts at: 6148 1493 Size: 1697 Fragments arranged by size: 2109 1697 1016 399 363 289 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3601 3677 1870 Size: 73 1658 76 4545 Fragments arranged by size: 4545 1658 76 73 AvrII C'CTAG_G Cuts at: 2029 2029 Size: 6352 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2485 3673 5128 618 Size: 325 217 627 698 1188 1455 1842 Fragments arranged by size: 1842 1455 1188 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 2472 2928 3208 3602 3671 1391 Size: 1081 456 280 394 69 4072 Fragments arranged by size: 4072 1081 456 394 280 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 928 Size: 971 1355 4026 Fragments arranged by size: 4026 1355 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4543 4577 1903 Size: 34 50 34 2522 34 3678 Fragments arranged by size: 3678 2522 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2408 4244 5454 2408 Size: 1836 1210 3306 Fragments arranged by size: 3306 1836 1210 BclI T'GATC_A Cuts at: 1548 2622 1548 Size: 1074 5278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3212 4963 136 Size: 107 122 71 631 605 1540 1751 1525 Fragments arranged by size: 1751 1540 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6347 6347 Size: 6352 BlpI GC'TnA_GC Cuts at: 3769 3769 Size: 6352 Bme1580I G_kGCm'C Cuts at: 1790 3320 4413 5569 1790 Size: 1530 1093 1156 2573 Fragments arranged by size: 2573 1530 1156 1093 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2062 2722 4083 Size: 416 53 83 399 24 645 660 1361 Cuts at: 4083 5041 442 Size: 958 1753 Fragments arranged by size: 1753 1361 958 660 645 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 3047 2122 Size: 925 5427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2092 2630 2747 3570 3616 5012 1239 Size: 853 538 117 823 46 1396 2579 Fragments arranged by size: 2579 1396 853 823 538 117 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 2998 3072 3185 4477 5342 5640 2525 Size: 473 74 113 1292 865 298 3237 Fragments arranged by size: 3237 1292 865 473 298 113 74 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 2168 2622 2723 2752 3173 5015 Size: 617 612 57 454 101 29 421 1842 Cuts at: 5015 882 Size: 2219 Fragments arranged by size: 2219 1842 617 612 454 421 101 57 29 BsaAI yAC'GTr Cuts at: 493 1204 4143 493 Size: 711 2939 2702 Fragments arranged by size: 2939 2702 711 BsaBI GATnn'nnATC Cuts at: 2396 6346 2396 Size: 3950 2402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3296 4785 5183 5457 5604 6015 3296 Size: 1489 398 274 147 411 3633 Fragments arranged by size: 3633 1489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6127 Fragments arranged by size: 6127 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3075 3182 1186 Size: 1889 107 4356 Fragments arranged by size: 4356 1889 107 BseYI C'CCAG_C Cuts at: 2595 2656 5555 6051 2595 Size: 61 2899 496 2896 Fragments arranged by size: 2899 2896 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2928 3320 3602 4413 4498 5569 2928 Size: 392 282 811 85 1071 3711 Fragments arranged by size: 3711 1071 811 392 282 85 BsmI GAATG_Cn' Cuts at: 3863 3956 3863 Size: 93 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2472 2928 3208 3320 3602 3671 4413 Size: 399 682 456 280 112 282 69 742 Cuts at: 4413 4498 5569 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 682 456 399 282 280 112 85 69 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 2170 4241 5971 2170 Size: 2071 1730 2551 Fragments arranged by size: 2551 2071 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2867 3239 3707 844 Size: 2023 372 468 3489 Fragments arranged by size: 3489 2023 468 372 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2393 4847 5021 65 Size: 1183 1145 2454 174 1396 Fragments arranged by size: 2454 1396 1183 1145 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 3449 4412 5424 3449 Size: 963 1012 4377 Fragments arranged by size: 4377 1012 963 BstAPI GCAn_nnn'nTGC Cuts at: 794 3324 794 Size: 2530 3822 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstYI r'GATC_y Cuts at: 1972 2241 2250 2292 2716 2844 3623 3682 4049 Size: 269 9 42 424 128 779 59 367 Cuts at: 4049 4434 4451 5903 5989 6001 6347 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 779 424 385 367 346 269 128 86 59 42 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2490 2518 2667 3036 2490 Size: 28 149 369 5806 Fragments arranged by size: 5806 369 149 28 BtgI C'CryG_G Cuts at: 513 1065 3210 513 Size: 552 2145 3655 Fragments arranged by size: 3655 2145 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2028 487 Size: 683 56 802 4811 Fragments arranged by size: 4811 802 683 56 BtsI GCAGTG_nn' Cuts at: 2346 2648 3868 4662 4682 2346 Size: 302 1220 794 20 4016 Fragments arranged by size: 4016 1220 794 302 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DraIII CAC_nnn'GTG Cuts at: 2548 3159 2548 Size: 611 5741 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317 Size: 54 1425 50 275 18 1969 889 1629 Cuts at: 6317 8 Size: 43 Fragments arranged by size: 1969 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2802 3167 4282 1353 Size: 975 474 365 1115 3423 Fragments arranged by size: 3423 1115 975 474 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2926 3600 2926 Size: 674 5678 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2092 2630 2747 2934 3570 3616 4415 Size: 393 460 538 117 187 636 46 799 Cuts at: 4415 5012 5799 6067 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 799 787 636 597 538 460 393 268 187 117 46 EcoNI CCTnn'n_nnAGG Cuts at: 2096 3138 3256 2096 Size: 1042 118 5192 Fragments arranged by size: 5192 1042 118 EcoO109I rG'GnC_Cy Cuts at: 2026 2368 2522 2635 2026 Size: 342 154 113 5743 Fragments arranged by size: 5743 342 154 113 EcoRI G'AATT_C Cuts at: 2509 3262 3661 4039 2509 Size: 753 399 378 4822 Fragments arranged by size: 4822 753 399 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3661 3661 Size: 6352 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2403 2408 2425 2435 2440 Size: 32 135 32 831 5 17 10 5 Cuts at: 2440 2457 2498 2530 4993 5025 5067 5099 1373 Size: 17 41 32 2463 32 42 32 2626 Fragments arranged by size: 2626 2463 831 135 42 41 32 32 32 32 32 17 17 10 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2014 3696 3942 669 Size: 988 281 76 1682 246 3079 Fragments arranged by size: 3079 1682 988 281 246 76 HindIII A'AGCT_T Cuts at: 748 2356 2946 748 Size: 1608 590 4154 Fragments arranged by size: 4154 1608 590 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 5466 5650 1305 Size: 936 3225 184 2007 Fragments arranged by size: 3225 2007 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2835 4310 4669 4828 518 Size: 648 1669 1475 359 159 2042 Fragments arranged by size: 2042 1669 1475 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3079 3594 3768 4445 5593 5838 1606 Size: 1473 515 174 677 1148 245 2120 Fragments arranged by size: 2120 1473 1148 677 515 245 174 NcoI C'CATG_G Cuts at: 513 1065 3210 513 Size: 552 2145 3655 Fragments arranged by size: 3655 2145 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2936 3710 5255 1132 Size: 97 1707 774 1545 2229 Fragments arranged by size: 2229 1707 1545 774 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 2327 3159 1108 Size: 1219 832 4301 Fragments arranged by size: 4301 1219 832 PfoI T'CCnGG_A Cuts at: 2320 2320 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2368 2635 2368 Size: 267 6085 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3564 3922 3564 Size: 358 5994 PspXI vC'TCGA_Gb Cuts at: 3601 3601 Size: 6352 PstI C_TGCA'G Cuts at: 830 3236 3704 830 Size: 2406 468 3478 Fragments arranged by size: 3478 2406 468 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 PvuII CAG'CTG Cuts at: 3079 3594 3079 Size: 515 5837 SacI G_AGCT'C Cuts at: 2928 3602 2928 Size: 674 5678 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SanDI GG'GwC_CC Cuts at: 2368 2368 Size: 6352 SapI GCTCTTCn'nnn_ Cuts at: 2802 2802 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3236 3704 3236 Size: 468 5884 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 6352 SfcI C'TryA_G Cuts at: 826 1045 2213 3232 3700 4835 5516 5707 826 Size: 219 1168 1019 468 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 1019 681 468 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 2977 3087 3164 3601 4456 Size: 197 926 561 473 110 77 437 855 Cuts at: 4456 5357 5619 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 855 561 473 437 262 197 110 77 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 2936 3710 1132 Size: 1804 774 3774 Fragments arranged by size: 3774 1804 774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 2034 1517 Size: 517 5835 StyI C'CwwG_G Cuts at: 513 1065 2029 3210 3791 513 Size: 552 964 1181 581 3074 Fragments arranged by size: 3074 1181 964 581 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2293 4379 4396 4549 4734 5179 325 Size: 1255 713 2086 17 153 185 445 1498 Fragments arranged by size: 2086 1498 1255 713 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4598 6310 96 Size: 274 80 33 51 1443 2621 1712 138 Fragments arranged by size: 2621 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6312 6344 6312 Size: 32 6320 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2159 2159 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 3601 1943 Size: 1658 4694 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3587 4481 3503 Size: 84 894 5374 Fragments arranged by size: 5374 894 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SfiI SfoI SrfI SwaI