(Circular) (Six-base) MAPSORT of: pFN21AB7307.seq Check: 2175 from: 1 to: 9823 pFN21AB7307 9823 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3116 3116 Size: 9823 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7633 278 Size: 53 83 186 936 6097 2468 Fragments arranged by size: 6097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3921 7166 1937 Size: 1984 3245 4594 Fragments arranged by size: 4594 3245 1984 Acc65I G'GTAC_C Cuts at: 4900 7144 4900 Size: 2244 7579 AclI AA'CG_TT Cuts at: 1563 7950 8323 1563 Size: 6387 373 3063 Fragments arranged by size: 6387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 3040 3285 6558 7886 9270 9538 1632 Size: 833 575 245 3273 1328 1384 268 1917 Fragments arranged by size: 3273 1917 1384 1328 833 575 268 245 AflII C'TTAA_G Cuts at: 820 1017 2674 820 Size: 197 1657 7969 Fragments arranged by size: 7969 1657 197 AflIII A'CryG_T Cuts at: 1203 3743 8722 9699 1203 Size: 2540 4979 977 1327 Fragments arranged by size: 4979 2540 1327 977 AgeI A'CCGG_T Cuts at: 8654 8654 Size: 9823 AhdI GACnn_n'nnGTC Cuts at: 5117 8552 5117 Size: 3435 6388 AleI CACnn'nnGTG Cuts at: 1166 3057 1166 Size: 1891 7932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9823 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6583 6615 6583 Size: 32 9791 AlwNI CAG_nnn'CTG Cuts at: 2414 2604 6335 6728 7566 9138 2414 Size: 190 3731 393 838 1572 3099 Fragments arranged by size: 3731 3099 1572 838 393 190 ApaLI G'TGCA_C Cuts at: 6797 7880 9036 6797 Size: 1083 1156 7584 Fragments arranged by size: 7584 1156 1083 ApoI r'AATT_y Cuts at: 1493 2087 2166 2417 2466 2552 3187 3574 4007 Size: 594 79 251 49 86 635 387 433 Cuts at: 4007 4332 4877 5154 5309 5444 5699 5879 6087 Size: 325 545 277 155 135 255 180 208 Cuts at: 6087 6348 6535 7132 7358 7510 9619 1493 Size: 261 187 597 226 152 2109 1697 Fragments arranged by size: 2109 1697 635 597 594 545 433 387 325 277 261 255 251 226 208 187 180 155 152 135 86 79 49 AseI AT'TA_AT Cuts at: 160 1032 2910 2979 8377 160 Size: 872 1878 69 5398 1606 Fragments arranged by size: 5398 1878 1606 872 69 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9823 AvaI C'yCGr_G Cuts at: 1870 1943 2274 7148 1870 Size: 73 331 4874 4545 Fragments arranged by size: 4874 4545 331 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5661 5694 5661 Size: 33 9790 BamHI G'GATC_C Cuts at: 7153 7520 7153 Size: 367 9456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2991 4900 5375 6718 7144 Size: 325 217 627 1204 1909 475 1343 426 Cuts at: 7144 8599 618 Size: 1455 1842 Fragments arranged by size: 1909 1842 1455 1343 1204 627 475 426 325 217 BanII G_rGCy'C Cuts at: 1391 4987 5638 6524 6953 7142 1391 Size: 3596 651 886 429 189 4072 Fragments arranged by size: 4072 3596 886 651 429 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 3621 928 Size: 971 438 1284 7130 Fragments arranged by size: 7130 1284 971 438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4034 4068 8014 8048 1903 Size: 34 2097 34 3946 34 3678 Fragments arranged by size: 3946 3678 2097 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2055 4772 6331 7715 8925 2055 Size: 2717 1559 1384 1210 2953 Fragments arranged by size: 2953 2717 1559 1384 1210 BclI T'GATC_A Cuts at: 1548 3364 1548 Size: 1816 8007 BfrBI ATG'CAT Cuts at: 6650 6650 Size: 9823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5373 8434 136 Size: 107 122 71 631 605 3701 3061 1525 Fragments arranged by size: 3701 3061 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9818 9818 Size: 9823 BlpI GC'TnA_GC Cuts at: 7240 7240 Size: 9823 Bme1580I G_kGCm'C Cuts at: 1790 2994 6801 7884 9040 1790 Size: 1204 3807 1083 1156 2573 Fragments arranged by size: 3807 2573 1204 1156 1083 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4321 7554 8512 Size: 416 53 83 399 24 2904 3233 958 Cuts at: 8512 442 Size: 1753 Fragments arranged by size: 3233 2904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4854 4854 Size: 9823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2547 2709 3454 4215 4635 4971 5076 Size: 989 319 162 745 761 420 336 105 Cuts at: 5076 5472 6585 6876 8483 1239 Size: 396 1113 291 1607 2579 Fragments arranged by size: 2579 1607 1113 989 761 745 420 396 336 319 291 162 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4190 5587 5658 5772 6867 7005 7948 8813 9111 Size: 1397 71 114 1095 138 943 865 298 Cuts at: 9111 4190 Size: 4902 Fragments arranged by size: 4902 1397 1095 943 865 298 138 114 71 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4839 8486 882 Size: 617 3340 3647 2219 Fragments arranged by size: 3647 3340 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7614 493 Size: 711 6410 2702 Fragments arranged by size: 6410 2702 711 BsaBI GATnn'nnATC Cuts at: 2586 3042 9817 2586 Size: 456 6775 2592 Fragments arranged by size: 6775 2592 456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7630 8012 8647 275 Size: 53 83 186 936 6097 382 635 1451 Fragments arranged by size: 6097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8256 8654 8928 9075 9486 8256 Size: 398 274 147 411 8593 Fragments arranged by size: 8593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 6583 6613 1178 Size: 30 165 30 798 30 4352 30 4388 Fragments arranged by size: 4388 4352 798 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2262 4910 1186 Size: 1076 2648 6099 Fragments arranged by size: 6099 2648 1076 BseYI C'CCAG_C Cuts at: 4588 6302 6624 6776 9026 9522 4588 Size: 1714 322 152 2250 496 4889 Fragments arranged by size: 4889 2250 1714 496 322 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4594 5796 6480 1163 Size: 573 2858 1202 684 4506 Fragments arranged by size: 4506 2858 1202 684 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7273 8034 9062 1540 Size: 275 186 5272 761 1028 2301 Fragments arranged by size: 5272 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2725 3054 4987 6801 6953 7884 7969 9040 Size: 590 329 1933 1814 152 931 85 1071 Cuts at: 9040 2135 Size: 2918 Fragments arranged by size: 2918 1933 1814 1071 931 590 329 152 85 BsmI GAATG_Cn' Cuts at: 4674 6372 7334 7427 4674 Size: 1698 962 93 7070 Fragments arranged by size: 7070 1698 962 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6687 6687 Size: 9823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2725 2994 3054 4987 5638 6524 Size: 399 345 590 269 60 1933 651 886 Cuts at: 6524 6801 6953 7142 7884 7969 9040 1391 Size: 277 152 189 742 85 1071 2174 Fragments arranged by size: 2174 1933 1071 886 742 651 590 399 345 277 269 189 152 85 60 BspEI T'CCGG_A Cuts at: 9486 9486 Size: 9823 BspHI T'CATG_A Cuts at: 2584 2653 2914 7712 9442 2584 Size: 69 261 4798 1730 2965 Fragments arranged by size: 4798 2965 1730 261 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 6811 6997 7178 844 Size: 2272 3695 186 181 3489 Fragments arranged by size: 3695 3489 2272 186 181 BsrBI CCG'CTC Cuts at: 1394 1823 2041 7710 1394 Size: 429 218 5669 3507 Fragments arranged by size: 5669 3507 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 8318 8492 65 Size: 1183 7070 174 1396 Fragments arranged by size: 7070 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8467 8654 1700 Size: 110 6657 187 2869 Fragments arranged by size: 6657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3881 5587 96 Size: 3785 1706 4332 Fragments arranged by size: 4332 3785 1706 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9823 BssSI C'ACGA_G Cuts at: 7883 8895 7883 Size: 1012 8811 BstAPI GCAn_nnn'nTGC Cuts at: 794 6503 794 Size: 5709 4114 BstBI TT'CG_AA Cuts at: 8627 8627 Size: 9823 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9823 BstXI CCAn_nnnn'nTGG Cuts at: 2297 6772 2297 Size: 4475 5348 BstYI r'GATC_y Cuts at: 1972 2031 2561 4823 5729 6309 7153 7520 7905 Size: 59 530 2262 906 580 844 367 385 Cuts at: 7905 7922 9374 9460 9472 9818 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2262 1977 1452 906 844 580 530 385 367 346 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 6526 6526 Size: 9823 BtgI C'CryG_G Cuts at: 513 1065 2004 3735 3845 6063 6704 6771 6941 Size: 552 939 1731 110 2218 641 67 170 Cuts at: 6941 513 Size: 3395 Fragments arranged by size: 3395 2218 1731 939 641 552 170 110 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9084 Fragments arranged by size: 9084 683 56 BtsI GCAGTG_nn' Cuts at: 2921 7339 8133 8153 2921 Size: 4418 794 20 4591 Fragments arranged by size: 4591 4418 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9788 DraI TTT'AAA Cuts at: 2818 3652 4869 5884 7127 2818 Size: 834 1217 1015 1243 5514 Fragments arranged by size: 5514 1243 1217 1015 834 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8830 809 Size: 507 7514 1802 Fragments arranged by size: 7514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 3848 4755 Size: 54 1425 50 275 18 594 1424 907 Cuts at: 4755 7270 8159 9788 8 Size: 2515 889 1629 43 Fragments arranged by size: 2515 1629 1425 1424 907 889 594 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7270 1537 Size: 275 5458 4090 Fragments arranged by size: 5458 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 2934 3762 4813 5883 6030 6169 7066 Size: 1020 561 828 1051 1070 147 139 897 Cuts at: 7066 7753 1353 Size: 687 3423 Fragments arranged by size: 3423 1070 1051 1020 897 828 687 561 147 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8410 8784 8930 387 Size: 1064 138 6821 374 146 1280 Fragments arranged by size: 6821 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4985 6951 4985 Size: 1966 7857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2465 2547 2709 3040 3285 3454 Size: 393 596 237 82 162 331 245 169 Cuts at: 3454 4215 4635 4971 5076 5472 6558 6585 6876 Size: 761 420 336 105 396 1086 27 291 Cuts at: 6876 7886 8483 9270 9538 1239 Size: 1010 597 787 268 1524 Fragments arranged by size: 1524 1086 1010 787 761 597 596 420 396 393 336 331 291 268 245 237 169 162 105 82 27 EcoNI CCTnn'n_nnAGG Cuts at: 2529 3761 5481 6391 2529 Size: 1232 1720 910 5961 Fragments arranged by size: 5961 1720 1232 910 EcoRI G'AATT_C Cuts at: 5699 7132 7510 5699 Size: 1433 378 8012 Fragments arranged by size: 8012 1433 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4234 6515 4234 Size: 2281 7542 FspI TGC'GCA Cuts at: 8329 8329 Size: 9823 HaeII r_GCGC'y Cuts at: 1678 8970 1678 Size: 7292 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2484 2516 3329 3361 4079 Size: 32 135 32 912 32 813 32 718 Cuts at: 4079 4111 5103 5135 5364 5396 5903 5935 6029 Size: 32 992 32 229 32 507 32 94 Cuts at: 6029 6061 6938 6970 8464 8496 8538 8570 1373 Size: 32 877 32 1494 32 42 32 2626 Fragments arranged by size: 2626 1494 992 912 877 813 718 507 229 135 94 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3337 5460 5559 7050 7167 7413 Size: 988 281 1399 2123 99 1491 117 246 Cuts at: 7413 669 Size: 3079 Fragments arranged by size: 3079 2123 1491 1399 988 281 246 117 99 HindIII A'AGCT_T Cuts at: 748 4244 748 Size: 3496 6327 HpaI GTT'AAC Cuts at: 5460 7413 5460 Size: 1953 7870 KpnI G_GTAC'C Cuts at: 4904 7148 4904 Size: 2244 7579 MfeI C'AATT_G Cuts at: 7422 7422 Size: 9823 MluI A'CGCG_T Cuts at: 9699 9699 Size: 9823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2418 3179 4053 4903 6343 6945 8937 9121 Size: 1113 761 874 850 1440 602 1992 184 Cuts at: 9121 1305 Size: 2007 Fragments arranged by size: 2007 1992 1440 1113 874 850 761 602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 3850 4757 10 Size: 54 1425 343 594 1424 907 5076 Fragments arranged by size: 5076 1425 1424 907 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 3057 6126 6770 7781 8140 8299 Size: 648 976 915 3069 644 1011 359 159 Cuts at: 8299 518 Size: 2042 Fragments arranged by size: 3069 2042 1011 976 915 648 644 359 159 MspA1I CmG'CkG Cuts at: 1606 5585 6578 7239 7916 9064 9309 1606 Size: 3979 993 661 677 1148 245 2120 Fragments arranged by size: 3979 2120 1148 993 677 661 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3735 3845 6063 6704 6771 513 Size: 552 939 1731 110 2218 641 67 3565 Fragments arranged by size: 3565 2218 1731 939 641 552 110 67 NdeI CA'TA_TG Cuts at: 387 2944 387 Size: 2557 7266 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9823 NotI GC'GGCC_GC Cuts at: 7270 7270 Size: 9823 NsiI A_TGCA'T Cuts at: 6652 6652 Size: 9823 NspI r_CATG'y Cuts at: 1132 1229 3378 3591 3747 7181 8726 1132 Size: 97 2149 213 156 3434 1545 2229 Fragments arranged by size: 3434 2229 2149 1545 213 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9823 PciI A'CATG_T Cuts at: 3743 8722 3743 Size: 4979 4844 PflMI CCAn_nnn'nTGG Cuts at: 1108 2966 6620 6728 1108 Size: 1858 3654 108 4203 Fragments arranged by size: 4203 3654 1858 108 PfoI T'CCnGG_A Cuts at: 2524 3431 4231 5449 2524 Size: 907 800 1218 6898 Fragments arranged by size: 6898 1218 907 800 PmeI GTTT'AAAC Cuts at: 7127 7127 Size: 9823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2670 2702 5015 5047 6583 6615 7892 7924 9405 Size: 32 2313 32 1536 32 1277 32 1481 Cuts at: 9405 9437 2670 Size: 32 3056 Fragments arranged by size: 3056 2313 1536 1481 1277 32 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9823 PsiI TTA'TAA Cuts at: 7393 7393 Size: 9823 PspXI vC'TCGA_Gb Cuts at: 2274 2274 Size: 9823 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2932 2964 2932 Size: 32 9791 PstI C_TGCA'G Cuts at: 830 4387 4951 6822 7175 830 Size: 3557 564 1871 353 3478 Fragments arranged by size: 3557 3478 1871 564 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9823 PvuII CAG'CTG Cuts at: 5585 6578 5585 Size: 993 8830 SacI G_AGCT'C Cuts at: 4987 6953 4987 Size: 1966 7857 SalI G'TCGA_C Cuts at: 1936 7165 1936 Size: 5229 4594 SapI GCTCTTCn'nnn_ Cuts at: 6030 6030 Size: 9823 SbfI CC_TGCA'GG Cuts at: 7175 7175 Size: 9823 ScaI AGT'ACT Cuts at: 4973 5125 8071 9783 4973 Size: 152 2946 1712 5013 Fragments arranged by size: 5013 2946 1712 152 SexAI A'CCwGG_T Cuts at: 5476 5476 Size: 9823 SfcI C'TryA_G Cuts at: 826 1045 2256 2373 4383 4947 6818 7171 8306 Size: 219 1211 117 2010 564 1871 353 1135 Cuts at: 8306 8987 9178 826 Size: 681 191 1471 Fragments arranged by size: 2010 1871 1471 1211 1135 681 564 353 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9823 SmaI CCC'GGG Cuts at: 1872 7150 1872 Size: 5278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2274 2674 4169 5602 5673 5787 Size: 197 926 331 400 1495 1433 71 114 Cuts at: 5787 6882 7020 7927 8828 9090 820 Size: 1095 138 907 901 262 1553 Fragments arranged by size: 1553 1495 1433 1095 926 907 901 400 331 262 197 138 114 71 SnaBI TAC'GTA Cuts at: 493 7614 493 Size: 7121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9823 SphI G_CATG'C Cuts at: 1132 3591 7181 1132 Size: 2459 3590 3774 Fragments arranged by size: 3774 3590 2459 SspI AAT'ATT Cuts at: 5 52 3635 4724 7747 5 Size: 47 3583 1089 3023 2081 Fragments arranged by size: 3583 3023 2081 1089 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9823 StyI C'CwwG_G Cuts at: 513 1065 2004 3735 3845 4765 5508 6063 6704 Size: 552 939 1731 110 920 743 555 641 Cuts at: 6704 6771 7262 513 Size: 67 491 3074 Fragments arranged by size: 3074 1731 939 920 743 641 555 552 491 110 67 SwaI ATTT'AAAT Cuts at: 2818 2818 Size: 9823 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7850 7867 8020 8205 8650 325 Size: 1255 6270 17 153 185 445 1498 Fragments arranged by size: 6270 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3491 3553 3881 Size: 274 80 33 51 1443 1514 62 328 Cuts at: 3881 4324 4734 4971 5123 5416 5587 6001 8069 Size: 443 410 237 152 293 171 414 2068 Cuts at: 8069 9781 96 Size: 1712 138 Fragments arranged by size: 2068 1712 1514 1443 443 414 410 328 293 274 237 171 152 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3486 3518 6272 6304 6702 6734 9783 9815 3486 Size: 32 2754 32 398 32 3049 32 3494 Fragments arranged by size: 3494 3049 2754 398 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2604 1316 Size: 1288 8535 XbaI T'CTAG_A Cuts at: 4450 7159 4450 Size: 2709 7114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2522 6621 2522 Size: 4099 5724 XhoI C'TCGA_G Cuts at: 1943 2274 1943 Size: 331 9492 XmaI C'CCGG_G Cuts at: 1870 7148 1870 Size: 5278 4545 XmnI GAAnn'nnTTC Cuts at: 2243 2367 2685 3543 4959 5082 5445 6352 6737 Size: 124 318 858 1416 123 363 907 385 Cuts at: 6737 7952 2243 Size: 1215 4114 Fragments arranged by size: 4114 1416 1215 907 858 385 363 318 124 123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7631 276 Size: 53 83 186 936 6097 2468 Fragments arranged by size: 6097 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI RsrII SacII SanDI SfiI SfoI SrfI