################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HaeII HpaI KasI KpnI MluI MroI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 173 GTATAC GT^[AC][GT]AC AccII 13 CGCG CG^CG AcyI 568 GGCGTC G[GA]^CG[CT]C Alw44I 1443 GTGCAC G^TGCAC AocI 475 CCTCAGG CC^T[ACGT]AGG ApaLI 1443 GTGCAC G^TGCAC AseI 1515 ATTAAT AT^TAAT AsnI 1515 ATTAAT AT^TAAT Asp700I 1027 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1275 TGGCCA TGG^CCA BanII 2195 GGGCTC G[GA]GC[CT]^C BclI 1860 TGATCA T^GATCA BlpI 1536 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1536 GCTAAGC GC^T[ACGT]AGC BsaAI 557 TACGTA [CT]AC^GT[GA] BsaHI 568 GGCGTC G[GA]^CG[CT]C BsgI 830 CTGCAC GTGCAG(16/14) BsmBI 61 GAGACG CGTCTC(1/5) BsmFI 1547 GTCCC GGGAC(10/14) BsmI 2210 GCATTC GAATGC(1/-1) BspHI 1863 TCATGA T^CATGA BspMI 2046 GCAGGT ACCTGC(4/8) BsrFI 1098 ACCGGC [GA]^CCGG[CT] Bst1107I 173 GTATAC GTA^TAC BstUI 13 CGCG CG^CG BstXI 2331 CCAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 173 GTATAC GTA^TAC Bsu36I 475 CCTCAGG CC^T[ACGT]AGG CelII 1536 GCTAAGC GC^T[ACGT]AGC CfoI 1155 GCGC GCG^C Cfr10I 1098 ACCGGC [GA]^CCGG[CT] DraII 471 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1460 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 475 CCTCAGG CC^T[ACGT]AGG EcoO109I 471 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 994 GATATC GAT^ATC FauI 14 GCGGG CCCGC(4/6) FbaI 1860 TGATCA T^GATCA HgaI 569 GCGTC GACGC(5/10) HhaI 1155 GCGC GCG^C Hin1I 568 GGCGTC G[GA]^CG[CT]C HinP1I 1155 GCGC G^CGC HincII 1224 GTCAAC GT[CT]^[GA]AC HindII 1224 GTCAAC GT[CT]^[GA]AC KspI 12 CCGCGG CCGC^GG MluNI 1275 TGGCCA TGG^CCA MscI 1275 TGGCCA TGG^CCA MvnI 13 CGCG CG^CG NcoI 1124 CCATGG C^CATGG PshAI 2085 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1515 ATTAAT AT^TAAT PstI 580 CTGCAG CTGCA^G RcaI 1863 TCATGA T^CATGA SacII 12 CCGCGG CCGC^GG SapI 1630 GAAGAGC GCTCTTC(1/4) ScaI 1110 AGTACT AGT^ACT SnaBI 557 TACGTA TAC^GTA XbaI 2907 TCTAGA T^CTAGA XcmI 1278 CCAGTTATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1027 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaII 40 GGACC G^G[AT]CC 1641 GGTCC BbsI 2576 GAAGAC GAAGAC(2/6) 2355 GTCTTC BpuAI 2576 GAAGAC GAAGAC(2/6) 2355 GTCTTC BsaBI 2776 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2905 GATCTAGATC BseRI 406 GAGGAG GAGGAG(10/8) 667 GAGGAG DsaI 12 CCGCGG C^C[GA][CT]GG 1124 CCATGG EaeI 1275 TGGCCA [CT]^GGCC[GA] 2771 TGGCCG HapII 38 CCGG C^CGG 1099 CCGG HindIII 73 AAGCTT A^AGCTT 442 AAGCTT HpaII 38 CCGG C^CGG 1099 CCGG MamI 2776 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2905 GATCTAGATC MfeI 1103 CAATTG C^AATTG 1542 CAATTG MslI 1119 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2057 CATTAAAATG MspI 38 CCGG C^CGG 1099 CCGG MunI 1103 CAATTG C^AATTG 1542 CAATTG NlaIV 40 GGACCC GG[ACGT]^[ACGT]CC 984 GGTTCC PleI 1989 GACTC GAGTC(4/5) 2302 GACTC SspI 752 AATATT AAT^ATT 1450 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BbvI BfaI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII