Restriction map for
pFN21AB8608
(Circular) (Six-base) MAPSORT of: pFN21AB8608.seq Check: 4622 from: 1 to: 7624
pFN21AB8608 7624 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5434 278
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 4967 1937
Size: 242 2788 4594
Fragments arranged by size:
4594 2788 242
Acc65I G'GTAC_C
Cuts at: 4945 4945
Size: 7624
AclI AA'CG_TT
Cuts at: 1563 5751 6124 1563
Size: 4188 373 3063
Fragments arranged by size:
4188 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4347 5687 7071 7339 1632
Size: 2715 1340 1384 268 1917
Fragments arranged by size:
2715 1917 1384 1340 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7427
AflIII A'CryG_T
Cuts at: 1203 6523 7500 1203
Size: 5320 977 1327
Fragments arranged by size:
5320 1327 977
AgeI A'CCGG_T
Cuts at: 6455 6455
Size: 7624
AhdI GACnn_n'nnGTC
Cuts at: 6353 6353
Size: 7624
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3384 1184
Size: 2200 5424
AlwNI CAG_nnn'CTG
Cuts at: 2231 2922 4417 4489 5367 6939 2231
Size: 691 1495 72 878 1572 2916
Fragments arranged by size:
2916 1572 1495 878 691 72
ApaLI G'TGCA_C
Cuts at: 3448 5681 6837 3448
Size: 2233 1156 4235
Fragments arranged by size:
4235 2233 1156
ApoI r'AATT_y
Cuts at: 1493 2008 2317 2361 2821 3763 4933 5159 5311
Size: 515 309 44 460 942 1170 226 152
Cuts at: 5311 7420 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1170 942 515 460 309 226
152 44
AseI AT'TA_AT
Cuts at: 160 1032 3521 6178 160
Size: 872 2489 2657 1606
Fragments arranged by size:
2657 2489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
AvaI C'yCGr_G
Cuts at: 1870 1943 4949 1870
Size: 73 3006 4545
Fragments arranged by size:
4545 3006 73
BamHI G'GATC_C
Cuts at: 4954 5321 4954
Size: 367 7257
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4945 6400 618
Size: 325 217 627 3158 1455 1842
Fragments arranged by size:
3158 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4204 4943 1391
Size: 2813 739 4072
Fragments arranged by size:
4072 2813 739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4353 4588 928
Size: 971 2454 235 3964
Fragments arranged by size:
3964 2454 971 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5815 5849 1903
Size: 34 3878 34 3678
Fragments arranged by size:
3878 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5516 6726 5516
Size: 1210 6414
BclI T'GATC_A
Cuts at: 1548 3865 1548
Size: 2317 5307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6235 136
Size: 107 122 71 631 605 4563 1525
Fragments arranged by size:
4563 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7619 7619
Size: 7624
BlpI GC'TnA_GC
Cuts at: 3542 5041 3542
Size: 1499 6125
Bme1580I G_kGCm'C
Cuts at: 1790 3452 3664 5685 6841 1790
Size: 1662 212 2021 1156 2573
Fragments arranged by size:
2573 2021 1662 1156 212
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2994 3837 5355
Size: 416 53 83 399 24 1577 843 1518
Cuts at: 5355 6313 442
Size: 958 1753
Fragments arranged by size:
1753 1577 1518 958 843 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3411 4070 4884 6284 1239
Size: 2172 659 814 1400 2579
Fragments arranged by size:
2579 2172 1400 814 659
Bpu10I CC'TnA_GC
Cuts at: 3392 3392
Size: 7624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3725 4055 4802 5749 6614 6912 3725
Size: 330 747 947 865 298 4437
Fragments arranged by size:
4437 947 865 747 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 4532 4598 6287 882
Size: 617 1506 1527 66 1689 2219
Fragments arranged by size:
2219 1689 1527 1506 617 66
BsaAI yAC'GTr
Cuts at: 493 1204 2564 5415 493
Size: 711 1360 2851 2702
Fragments arranged by size:
2851 2702 1360 711
BsaBI GATnn'nnATC
Cuts at: 4785 4914 7618 4785
Size: 129 2704 4791
Fragments arranged by size:
4791 2704 129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 5431 5813 6448
Size: 53 83 186 936 1041 2857 382 635
Cuts at: 6448 275
Size: 1451
Fragments arranged by size:
2857 1451 1041 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6057 6455 6729 6876 7287 6057
Size: 398 274 147 411 6394
Fragments arranged by size:
6394 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2682 2712 2732 2762 2887
Size: 30 165 30 1279 30 20 30 125
Cuts at: 2887 2917 3210 3240 1178
Size: 30 293 30 5562
Fragments arranged by size:
5562 1279 293 165 125 30 30 30
30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2687 1186
Size: 1240 261 6123
Fragments arranged by size:
6123 1240 261
BseYI C'CCAG_C
Cuts at: 2049 3828 6827 7323 2049
Size: 1779 2999 496 2350
Fragments arranged by size:
2999 2350 1779 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2820 1163
Size: 573 1084 5967
Fragments arranged by size:
5967 1084 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5074 5835 6863 1540
Size: 275 186 3073 761 1028 2301
Fragments arranged by size:
3073 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 3452 4773 5685 5770 6841 2140
Size: 1312 1321 912 85 1071 2923
Fragments arranged by size:
2923 1321 1312 1071 912 85
BsmI GAATG_Cn'
Cuts at: 4215 5135 5228 4215
Size: 920 93 6611
Fragments arranged by size:
6611 920 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 7624
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 3452 3664 4204 4773 4943 5685
Size: 399 350 1312 212 540 569 170 742
Cuts at: 5685 5770 6841 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1312 1071 742 569 540 399 350
212 170 85
BspEI T'CCGG_A
Cuts at: 7287 7287
Size: 7624
BspHI T'CATG_A
Cuts at: 3868 5513 7243 3868
Size: 1645 1730 4249
Fragments arranged by size:
4249 1730 1645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4042 4979 844
Size: 3198 937 3489
Fragments arranged by size:
3489 3198 937
BsrBI CCG'CTC
Cuts at: 1394 1823 5511 1394
Size: 429 3688 3507
Fragments arranged by size:
3688 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 3555 4689 6119 6293 65
Size: 1183 1363 944 1134 1430 174 1396
Fragments arranged by size:
1430 1396 1363 1183 1134 944 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3103 6268 6455 1700
Size: 110 1293 3165 187 2869
Fragments arranged by size:
3165 2869 1293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7624
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7624
BssSI C'ACGA_G
Cuts at: 5684 6696 5684
Size: 1012 6612
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7624
BstBI TT'CG_AA
Cuts at: 6428 6428
Size: 7624
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7624
BstXI CCAn_nnnn'nTGG
Cuts at: 4343 4343
Size: 7624
BstYI r'GATC_y
Cuts at: 1972 4024 4116 4822 4897 4909 4954 5321 5706
Size: 2052 92 706 75 12 45 367 385
Cuts at: 5706 5723 7175 7261 7273 7619 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2052 1977 1452 706 385 367 346 92
86 75 45 17 12 12
BstZ17I GTA'TAC
Cuts at: 2180 2180
Size: 7624
Bsu36I CC'TnA_GG
Cuts at: 2481 2481
Size: 7624
BtgI C'CryG_G
Cuts at: 513 1065 2017 3129 513
Size: 552 952 1112 5008
Fragments arranged by size:
5008 1112 952 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6885
Fragments arranged by size:
6885 683 56
BtsI GCAGTG_nn'
Cuts at: 5140 5934 5954 5140
Size: 794 20 6810
Fragments arranged by size:
6810 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7589
DraI TTT'AAA
Cuts at: 2466 2767 4428 4928 2466
Size: 301 1661 500 5162
Fragments arranged by size:
5162 1661 500 301
DraIII CAC_nnn'GTG
Cuts at: 3470 3470
Size: 7624
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6631 809
Size: 507 5315 1802
Fragments arranged by size:
5315 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4776 5071
Size: 54 1425 50 275 18 1450 1496 295
Cuts at: 5071 5960 7589 8
Size: 889 1629 43
Fragments arranged by size:
1629 1496 1450 1425 889 295 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5071 1537
Size: 275 3259 4090
Fragments arranged by size:
4090 3259 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3630 3740 3948 4242 4839 5554 1353
Size: 1044 1233 110 208 294 597 715 3423
Fragments arranged by size:
3423 1233 1044 715 597 294 208 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6211 6585 6731 387
Size: 1064 138 4622 374 146 1280
Fragments arranged by size:
4622 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3411 4070 4347 4884 5687 6284 7071
Size: 393 1779 659 277 537 803 597 787
Cuts at: 7071 7339 1239
Size: 268 1524
Fragments arranged by size:
1779 1524 803 787 659 597 537 393
277 268
EcoO109I rG'GnC_Cy
Cuts at: 2477 2477
Size: 7624
EcoRI G'AATT_C
Cuts at: 4933 5311 4933
Size: 378 7246
EcoRV GAT'ATC
Cuts at: 3001 3001
Size: 7624
FspI TGC'GCA
Cuts at: 6130 6130
Size: 7624
HaeII r_GCGC'y
Cuts at: 1678 6771 1678
Size: 5093 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3850 3882 6265 6297 6339
Size: 32 135 32 2278 32 2383 32 42
Cuts at: 6339 6371 1373
Size: 32 2626
Fragments arranged by size:
2626 2383 2278 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 4968 5214 669
Size: 988 281 1293 1737 246 3079
Fragments arranged by size:
3079 1737 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2078 2447 748
Size: 1330 369 5925
Fragments arranged by size:
5925 1330 369
HpaI GTT'AAC
Cuts at: 5214 5214
Size: 7624
KpnI G_GTAC'C
Cuts at: 4949 4949
Size: 7624
MfeI C'AATT_G
Cuts at: 3108 3547 5223 3108
Size: 439 1676 5509
Fragments arranged by size:
5509 1676 439
MluI A'CGCG_T
Cuts at: 7500 7500
Size: 7624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2791 3493 4054 4707 6738 6922 1305
Size: 1486 702 561 653 2031 184 2007
Fragments arranged by size:
2031 2007 1486 702 653 561 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3282 10
Size: 54 1425 343 1450 4352
Fragments arranged by size:
4352 1450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 4066 5582 5941 6100 518
Size: 648 1962 938 1516 359 159 2042
Fragments arranged by size:
2042 1962 1516 938 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2433 3278 4294 4669 5040 5717 6865
Size: 413 414 845 1016 375 371 677 1148
Cuts at: 6865 7110 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1016 845 677 414 413 375
371 245
NcoI C'CATG_G
Cuts at: 513 1065 3129 513
Size: 552 2064 5008
Fragments arranged by size:
5008 2064 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7624
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7624
NotI GC'GGCC_GC
Cuts at: 5071 5071
Size: 7624
NspI r_CATG'y
Cuts at: 1132 1229 4982 6527 1132
Size: 97 3753 1545 2229
Fragments arranged by size:
3753 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7624
PciI A'CATG_T
Cuts at: 6523 6523
Size: 7624
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7624
PmeI GTTT'AAAC
Cuts at: 4928 4928
Size: 7624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3375 3407 5693 5725 7206 7238 3375
Size: 32 2286 32 1481 32 3761
Fragments arranged by size:
3761 2286 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4094 1613
Size: 2481 5143
PsiI TTA'TAA
Cuts at: 3908 3924 5194 3908
Size: 16 1270 6338
Fragments arranged by size:
6338 1270 16
PstI C_TGCA'G
Cuts at: 830 2589 4976 830
Size: 1759 2387 3478
Fragments arranged by size:
3478 2387 1759
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7624
PvuII CAG'CTG
Cuts at: 3278 4294 4669 3278
Size: 1016 375 6233
Fragments arranged by size:
6233 1016 375
SacII CC_GC'GG
Cuts at: 2020 2020
Size: 7624
SalI G'TCGA_C
Cuts at: 1936 4966 1936
Size: 3030 4594
SapI GCTCTTCn'nnn_
Cuts at: 3630 3630
Size: 7624
SbfI CC_TGCA'GG
Cuts at: 4976 4976
Size: 7624
ScaI AGT'ACT
Cuts at: 3117 5872 7584 3117
Size: 2755 1712 3157
Fragments arranged by size:
3157 2755 1712
SfcI C'TryA_G
Cuts at: 826 1045 2585 2867 4469 4972 6107 6788 6979
Size: 219 1540 282 1602 503 1135 681 191
Cuts at: 6979 826
Size: 1471
Fragments arranged by size:
1602 1540 1471 1135 681 503 282 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7624
SmaI CCC'GGG
Cuts at: 1872 4951 1872
Size: 3079 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3704 4034 4817 5728 6629 6891
Size: 197 926 1761 330 783 911 901 262
Cuts at: 6891 820
Size: 1553
Fragments arranged by size:
1761 1553 926 911 901 783 330 262
197
SnaBI TAC'GTA
Cuts at: 493 2564 5415 493
Size: 2071 2851 2702
Fragments arranged by size:
2851 2702 2071
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7624
SphI G_CATG'C
Cuts at: 1132 4982 1132
Size: 3850 3774
SspI AAT'ATT
Cuts at: 5 52 2759 3457 5548 5
Size: 47 2707 698 2091 2081
Fragments arranged by size:
2707 2091 2081 698 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7624
StyI C'CwwG_G
Cuts at: 513 1065 2798 3129 3649 5063 513
Size: 552 1733 331 520 1414 3074
Fragments arranged by size:
3074 1733 1414 552 520 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3389 4556 5651 5668 5821 6006
Size: 1255 924 885 1167 1095 17 153 185
Cuts at: 6006 6451 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1167 1095 924 885 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2966 3115 5870
Size: 274 80 33 51 1443 989 149 2755
Cuts at: 5870 7582 96
Size: 1712 138
Fragments arranged by size:
2755 1712 1443 989 274 149 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2887 2919 3210 3242 7584 7616 2887
Size: 32 291 32 4342 32 2895
Fragments arranged by size:
4342 2895 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7624
XbaI T'CTAG_A
Cuts at: 4912 4960 4912
Size: 48 7576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3290 3290
Size: 7624
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7624
XmaI C'CCGG_G
Cuts at: 1870 4949 1870
Size: 3079 4545
XmnI GAAnn'nnTTC
Cuts at: 3036 5753 3036
Size: 2717 4907
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5432 276
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SexAI SfiI SfoI SrfI SwaI