Restriction map for
pFN21AB8608
(Linear) (Six-base) MAPSORT of: KIBB8608.seq Check: 301 from: 1 to: 2919
KIBB8608.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AccI GT'mk_AC
Cuts at: 0 174 2919
Size: 174 2745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2342 2919
Size: 2342 577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1379 2919
Size: 1379 1540
AlwNI CAG_nnn'CTG
Cuts at: 0 226 917 2412 2484 2919
Size: 226 691 1495 72 435
Fragments arranged by size:
1495 691 435 226 72
ApaLI G'TGCA_C
Cuts at: 0 1443 2919
Size: 1443 1476
ApoI r'AATT_y
Cuts at: 0 3 312 356 816 1758 2919
Size: 3 309 44 460 942 1161
Fragments arranged by size:
1161 942 460 309 44 3
AseI AT'TA_AT
Cuts at: 0 1516 2919
Size: 1516 1403
BanII G_rGCy'C
Cuts at: 0 2199 2919
Size: 2199 720
BbsI GAAGACnn'nnnn_
Cuts at: 0 2348 2583 2919
Size: 2348 235 336
Fragments arranged by size:
2348 336 235
BclI T'GATC_A
Cuts at: 0 1860 2919
Size: 1860 1059
BlpI GC'TnA_GC
Cuts at: 0 1537 2919
Size: 1537 1382
Bme1580I G_kGCm'C
Cuts at: 0 1447 1659 2919
Size: 1447 212 1260
Fragments arranged by size:
1447 1260 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 989 1832 2919
Size: 989 843 1087
Fragments arranged by size:
1087 989 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 2065 2879 2919
Size: 1406 659 814 40
Fragments arranged by size:
1406 814 659 40
Bpu10I CC'TnA_GC
Cuts at: 0 1387 2919
Size: 1387 1532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1720 2050 2797 2919
Size: 1720 330 747 122
Fragments arranged by size:
1720 747 330 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 2527 2593 2919
Size: 1000 1527 66 326
Fragments arranged by size:
1527 1000 326 66
BsaAI yAC'GTr
Cuts at: 0 559 2919
Size: 559 2360
BsaBI GATnn'nnATC
Cuts at: 0 2780 2909 2919
Size: 2780 129 10
Fragments arranged by size:
2780 129 10
BsaHI Gr'CG_yC
Cuts at: 0 569 2919
Size: 569 2350
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 677 707 727 757 882 912 1205 1235
Size: 677 30 20 30 125 30 293 30
Cuts at: 1235 2919
Size: 1684
Fragments arranged by size:
1684 677 293 125 30 30 30 30
20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 682 2919
Size: 421 261 2237
Fragments arranged by size:
2237 421 261
BseYI C'CCAG_C
Cuts at: 0 44 1823 2919
Size: 44 1779 1096
Fragments arranged by size:
1779 1096 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 2919
Size: 815 2104
BsiHKAI G_wGCw'C
Cuts at: 0 135 1447 2768 2919
Size: 135 1312 1321 151
Fragments arranged by size:
1321 1312 151 135
BsmI GAATG_Cn'
Cuts at: 0 2210 2919
Size: 2210 709
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 2919
Size: 55 2864
Bsp1286I G_dGCh'C
Cuts at: 0 135 1447 1659 2199 2768 2919
Size: 135 1312 212 540 569 151
Fragments arranged by size:
1312 569 540 212 151 135
BspHI T'CATG_A
Cuts at: 0 1863 2919
Size: 1863 1056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2037 2919
Size: 2037 882
BsrDI GCAATG_nn'
Cuts at: 0 606 1550 2684 2919
Size: 606 944 1134 235
Fragments arranged by size:
1134 944 606 235
BsrFI r'CCGG_y
Cuts at: 0 1098 2919
Size: 1098 1821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2338 2919
Size: 2338 581
BstYI r'GATC_y
Cuts at: 0 2019 2111 2817 2892 2904 2919
Size: 2019 92 706 75 12 15
Fragments arranged by size:
2019 706 92 75 15 12
BstZ17I GTA'TAC
Cuts at: 0 175 2919
Size: 175 2744
Bsu36I CC'TnA_GG
Cuts at: 0 476 2919
Size: 476 2443
BtgI C'CryG_G
Cuts at: 0 12 1124 2919
Size: 12 1112 1795
Fragments arranged by size:
1795 1112 12
DraI TTT'AAA
Cuts at: 0 461 762 2423 2919
Size: 461 301 1661 496
Fragments arranged by size:
1661 496 461 301
DraIII CAC_nnn'GTG
Cuts at: 0 1465 2919
Size: 1465 1454
EaeI y'GGCC_r
Cuts at: 0 1275 2771 2919
Size: 1275 1496 148
Fragments arranged by size:
1496 1275 148
EarI CTCTTCn'nnn_
Cuts at: 0 392 1625 1735 1943 2237 2834 2919
Size: 392 1233 110 208 294 597 85
Fragments arranged by size:
1233 597 392 294 208 110 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 2065 2342 2879 2919
Size: 1406 659 277 537 40
Fragments arranged by size:
1406 659 537 277 40
EcoO109I rG'GnC_Cy
Cuts at: 0 472 2919
Size: 472 2447
EcoRV GAT'ATC
Cuts at: 0 996 2919
Size: 996 1923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1845 1877 2919
Size: 1845 32 1042
Fragments arranged by size:
1845 1042 32
HincII GTy'rAC
Cuts at: 0 1226 2919
Size: 1226 1693
HindIII A'AGCT_T
Cuts at: 0 73 442 2919
Size: 73 369 2477
Fragments arranged by size:
2477 369 73
MfeI C'AATT_G
Cuts at: 0 1103 1542 2919
Size: 1103 439 1377
Fragments arranged by size:
1377 1103 439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1488 2049 2702 2919
Size: 786 702 561 653 217
Fragments arranged by size:
786 702 653 561 217
MscI TGG'CCA
Cuts at: 0 1277 2919
Size: 1277 1642
MslI CAynn'nnrTG
Cuts at: 0 1123 2061 2919
Size: 1123 938 858
Fragments arranged by size:
1123 938 858
MspA1I CmG'CkG
Cuts at: 0 14 428 1273 2289 2664 2919
Size: 14 414 845 1016 375 255
Fragments arranged by size:
1016 845 414 375 255 14
NcoI C'CATG_G
Cuts at: 0 1124 2919
Size: 1124 1795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1370 1402 2919
Size: 1370 32 1517
Fragments arranged by size:
1517 1370 32
PshAI GACnn'nnGTC
Cuts at: 0 2089 2919
Size: 2089 830
PsiI TTA'TAA
Cuts at: 0 1903 1919 2919
Size: 1903 16 1000
Fragments arranged by size:
1903 1000 16
PstI C_TGCA'G
Cuts at: 0 584 2919
Size: 584 2335
PvuII CAG'CTG
Cuts at: 0 1273 2289 2664 2919
Size: 1273 1016 375 255
Fragments arranged by size:
1273 1016 375 255
SacII CC_GC'GG
Cuts at: 0 15 2919
Size: 15 2904
SapI GCTCTTCn'nnn_
Cuts at: 0 1625 2919
Size: 1625 1294
ScaI AGT'ACT
Cuts at: 0 1112 2919
Size: 1112 1807
SfcI C'TryA_G
Cuts at: 0 580 862 2464 2919
Size: 580 282 1602 455
Fragments arranged by size:
1602 580 455 282
SmlI C'TyrA_G
Cuts at: 0 1699 2029 2812 2919
Size: 1699 330 783 107
Fragments arranged by size:
1699 783 330 107
SnaBI TAC'GTA
Cuts at: 0 559 2919
Size: 559 2360
SspI AAT'ATT
Cuts at: 0 754 1452 2919
Size: 754 698 1467
Fragments arranged by size:
1467 754 698
StyI C'CwwG_G
Cuts at: 0 793 1124 1644 2919
Size: 793 331 520 1275
Fragments arranged by size:
1275 793 520 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 1384 2551 2919
Size: 499 885 1167 368
Fragments arranged by size:
1167 885 499 368
TatI w'GTAC_w
Cuts at: 0 961 1110 2919
Size: 961 149 1809
Fragments arranged by size:
1809 961 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 882 914 1205 1237 2919
Size: 882 32 291 32 1682
Fragments arranged by size:
1682 882 291 32 32
XbaI T'CTAG_A
Cuts at: 0 2907 2919
Size: 2907 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1285 2919
Size: 1285 1634
XmnI GAAnn'nnTTC
Cuts at: 0 1031 2919
Size: 1031 1888
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AseI BanII
BbsI BclI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BstXI BstYI BstZ17I Bsu36I BtgI DraI DraIII EaeI
EarI Eco57MI EcoO109I EcoRV Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI PpiI PshAI PsiI
PstI PvuII SacII SapI ScaI SfcI SmlI SnaBI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BmgBI BmtI BplI BsaWI BsiEI BsiWI
BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI ZraI