(Linear) (Six-base) MAPSORT of: KIEE6449.seq Check: 6945 from: 1 to: 1602 KIEE6449.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 428 1430 1602 Size: 428 1002 172 Fragments arranged by size: 1002 428 172 Acc65I G'GTAC_C Cuts at: 0 1451 1602 Size: 1451 151 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1283 1602 Size: 1283 319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 440 1602 Size: 440 1162 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2 34 1602 Size: 2 32 1568 Fragments arranged by size: 1568 32 2 AlwNI CAG_nnn'CTG Cuts at: 0 119 1602 Size: 119 1483 ApoI r'AATT_y Cuts at: 0 262 325 868 941 1602 Size: 262 63 543 73 661 Fragments arranged by size: 661 543 262 73 63 AscI GG'CGCG_CC Cuts at: 0 1412 1602 Size: 1412 190 AseI AT'TA_AT Cuts at: 0 884 1602 Size: 884 718 BanI G'GyrC_C Cuts at: 0 99 416 1451 1602 Size: 99 317 1035 151 Fragments arranged by size: 1035 317 151 99 BbsI GAAGACnn'nnnn_ Cuts at: 0 813 1602 Size: 813 789 BclI T'GATC_A Cuts at: 0 365 410 1188 1602 Size: 365 45 778 414 Fragments arranged by size: 778 414 365 45 BfrBI ATG'CAT Cuts at: 0 754 1602 Size: 754 848 BlpI GC'TnA_GC Cuts at: 0 124 1602 Size: 124 1478 Bme1580I G_kGCm'C Cuts at: 0 104 553 1602 Size: 104 449 1049 Fragments arranged by size: 1049 449 104 BmgBI CAC'GTC Cuts at: 0 1473 1602 Size: 1473 129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 914 1250 1317 1602 Size: 208 706 336 67 285 Fragments arranged by size: 706 336 285 208 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 537 1602 Size: 537 1065 BsaI GGTCTCn'nnnn_ Cuts at: 0 939 1602 Size: 939 663 BsaWI w'CCGG_w Cuts at: 0 1544 1602 Size: 1544 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 556 586 1217 1247 1602 Size: 556 30 631 30 355 Fragments arranged by size: 631 556 355 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 1602 Size: 46 1556 BseYI C'CCAG_C Cuts at: 0 279 1602 Size: 279 1323 Bsp1286I G_dGCh'C Cuts at: 0 104 553 1602 Size: 104 449 1049 Fragments arranged by size: 1049 449 104 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 428 1430 1602 Size: 428 1002 172 Fragments arranged by size: 1002 428 172 BsrDI GCAATG_nn' Cuts at: 0 1158 1602 Size: 1158 444 BsrGI T'GTAC_A Cuts at: 0 200 1602 Size: 200 1402 BssHII G'CGCG_C Cuts at: 0 1412 1602 Size: 1412 190 BstAPI GCAn_nnn'nTGC Cuts at: 0 700 1602 Size: 700 902 BstEII G'GTnAC_C Cuts at: 0 1087 1602 Size: 1087 515 BstYI r'GATC_y Cuts at: 0 738 1602 Size: 738 864 BtgI C'CryG_G Cuts at: 0 1128 1163 1602 Size: 1128 35 439 Fragments arranged by size: 1128 439 35 DraI TTT'AAA Cuts at: 0 1524 1602 Size: 1524 78 DraIII CAC_nnn'GTG Cuts at: 0 171 557 1602 Size: 171 386 1045 Fragments arranged by size: 1045 386 171 EaeI y'GGCC_r Cuts at: 0 2 35 1373 1490 1602 Size: 2 33 1338 117 112 Fragments arranged by size: 1338 117 112 33 2 EarI CTCTTCn'nnn_ Cuts at: 0 206 1602 Size: 206 1396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 914 1250 1283 1317 1602 Size: 208 706 336 33 34 285 Fragments arranged by size: 706 336 285 208 34 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 1342 1602 Size: 1342 260 EcoO109I rG'GnC_Cy Cuts at: 0 147 405 1602 Size: 147 258 1197 Fragments arranged by size: 1197 258 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 917 949 1602 Size: 917 32 653 Fragments arranged by size: 917 653 32 KpnI G_GTAC'C Cuts at: 0 1455 1602 Size: 1455 147 MfeI C'AATT_G Cuts at: 0 965 1602 Size: 965 637 MscI TGG'CCA Cuts at: 0 4 37 1602 Size: 4 33 1565 Fragments arranged by size: 1565 33 4 MslI CAynn'nnrTG Cuts at: 0 422 759 1168 1602 Size: 422 337 409 434 Fragments arranged by size: 434 422 409 337 MspA1I CmG'CkG Cuts at: 0 33 1365 1602 Size: 33 1332 237 Fragments arranged by size: 1332 237 33 NcoI C'CATG_G Cuts at: 0 1128 1163 1602 Size: 1128 35 439 Fragments arranged by size: 1128 439 35 NdeI CA'TA_TG Cuts at: 0 1147 1602 Size: 1147 455 NsiI A_TGCA'T Cuts at: 0 756 1602 Size: 756 846 NspI r_CATG'y Cuts at: 0 132 427 642 1602 Size: 132 295 215 960 Fragments arranged by size: 960 295 215 132 PasI CC'CwG_GG Cuts at: 0 71 1602 Size: 71 1531 PflMI CCAn_nnn'nTGG Cuts at: 0 171 174 248 1467 1602 Size: 171 3 74 1219 135 Fragments arranged by size: 1219 171 135 74 3 PpuMI rG'GwC_Cy Cuts at: 0 147 1602 Size: 147 1455 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 628 660 1602 Size: 628 32 942 Fragments arranged by size: 942 628 32 PvuII CAG'CTG Cuts at: 0 33 1602 Size: 33 1569 SexAI A'CCwGG_T Cuts at: 0 167 1447 1602 Size: 167 1280 155 Fragments arranged by size: 1280 167 155 SmlI C'TyrA_G Cuts at: 0 516 1602 Size: 516 1086 SphI G_CATG'C Cuts at: 0 132 427 1602 Size: 132 295 1175 Fragments arranged by size: 1175 295 132 StuI AGG'CCT Cuts at: 0 139 1602 Size: 139 1463 StyI C'CwwG_G Cuts at: 0 1128 1163 1466 1602 Size: 1128 35 303 136 Fragments arranged by size: 1128 303 136 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1129 1374 1602 Size: 1129 245 228 Fragments arranged by size: 1129 245 228 TatI w'GTAC_w Cuts at: 0 200 1602 Size: 200 1402 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 543 575 1217 1249 1602 Size: 543 32 642 32 353 Fragments arranged by size: 642 543 353 32 32 XbaI T'CTAG_A Cuts at: 0 331 1602 Size: 331 1271 XmnI GAAnn'nnTTC Cuts at: 0 220 314 1602 Size: 220 94 1288 Fragments arranged by size: 1288 220 94 Enzymes that do cut: AarI Acc65I AcuI AlfI AloI AlwNI ApoI AscI AseI BanI BbsI BclI BfrBI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI Bsp1286I BspMI BsrDI BsrGI BssHII BstAPI BstEII BstYI BtgI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I KpnI MfeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI PpuMI PsrI PvuII SexAI SmlI SphI StuI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BglI BglII BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XcmI XhoI XmaI ZraI