Restriction map for
pFN21AB7885
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflIII 1148 ACATGT A^C[GA][CT]GT
AocI 1630 CCTCAGG CC^T[ACGT]AGG
BalI 398 TGGCCA TGG^CCA
BamHI 240 GGATCC G^GATCC
BanI 1304 GGTGCC G^G[CT][GA]CC
BclI 222 TGATCA T^GATCA
BcnI 23 CCGGG CC^[GC]GG
BglII 1108 AGATCT A^GATCT
BsgI 1187 GTGCAG GTGCAG(16/14)
Bsp1407I 1314 TGTACA T^GTACA
BspHI 627 TCATGA T^CATGA
BspLU11I 1148 ACATGT A^CATGT
BsrBI 904 GAGCGG CCGCTC(-3/-3)
BsrDI 1065 CATTGC GCAATG(2/0)
BsrGI 1314 TGTACA T^GTACA
BstAPI 1251 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 830 TTCGAA TT^CGAA
BstEII 975 GGTCACC G^GT[ACGT]ACC
BstF5I 78 GGATG GGATG(2/0)
BstPI 975 GGTCACC G^GT[ACGT]ACC
BstXI 1669 CCAAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1630 CCTCAGG CC^T[ACGT]AGG
Cac8I 480 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 564 GCGC GCG^C
EaeI 398 TGGCCA [CT]^GGCC[GA]
Eco81I 1630 CCTCAGG CC^T[ACGT]AGG
EcoNI 1654 CCTATTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 975 GGTCACC G^GT[ACGT]ACC
EcoRI 705 GAATTC G^AATTC
FbaI 222 TGATCA T^GATCA
FokI 78 GGATG GGATG(9/13)
HapII 23 CCGG C^CGG
HhaI 564 GCGC GCG^C
HinP1I 564 GCGC G^CGC
HincII 1174 GTTGAC GT[CT]^[GA]AC
HindII 1174 GTTGAC GT[CT]^[GA]AC
HpaII 23 CCGG C^CGG
MaeII 104 ACGT A^CGT
MfeI 113 CAATTG C^AATTG
MluNI 398 TGGCCA TGG^CCA
MscI 398 TGGCCA TGG^CCA
MspA1I 31 CCGCTG C[AC]G^C[GT]G
MspI 23 CCGG C^CGG
MunI 113 CAATTG C^AATTG
NciI 23 CCGGG CC^[GC]GG
NspI 1148 ACATGT [GA]CATG^[CT]
NspV 830 TTCGAA TT^CGAA
PstI 371 CTGCAG CTGCA^G
RcaI 627 TCATGA T^CATGA
SapI 1117 GAAGAGC GCTCTTC(1/4)
SfcI 371 CTGCAG C^T[GA][CT]AG
SfuI 830 TTCGAA TT^CGAA
SpeI 823 ACTAGT A^CTAGT
SspBI 1314 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 1501 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1543 GACTGCTTGTC
Alw44I 37 GTGCAC G^TGCAC
45 GTGCAC
ApaLI 37 GTGCAC G^TGCAC
45 GTGCAC
Asp700I 63 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAAGGCTTTC
AspEI 1501 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1543 GACTGCTTGTC
AspHI 37 GTGCAC G[AT]GC[AT]^C
45 GTGCAC
BmyI 37 GTGCAC G[AGT]GC[ACT]^C
45 GTGCAC
BsiHKAI 37 GTGCAC G[AT]GC[AT]^C
45 GTGCAC
BsiYI 1384 CCCCACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1654 CCTATTAAAGG
BslI 1384 CCCCACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1654 CCTATTAAAGG
BsmAI 1051 GAGAC GTCTC(1/5)
1441 GTCTC
Bsp1286I 37 GTGCAC G[AGT]GC[ACT]^C
45 GTGCAC
Eam1105I 1501 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1543 GACTGCTTGTC
EarI 127 GAAGAG CTCTTC(1/4)
1117 GAAGAG
EcoT14I 401 CCAAGG C^C[AT][AT]GG
731 CCAAGG
FauI 309 GCGGG CCCGC(4/6)
906 GCGGG
HphI 504 TCACC GGTGA(8/7)
977 TCACC
Ksp632I 127 GAAGAG CTCTTC(1/4)
1117 GAAGAG
PleI 655 GACTC GAGTC(4/5)
856 GAGTC
PpuMI 734 AGGACCT [GA]G^G[AT]CC[CT]
835 AGGACCC
PshAI 837 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1582 GACATCAGTC
SmlI 901 CTTGAG C^T[CT][GA]AG
1433 CTCAAG
StyI 401 CCAAGG C^C[AT][AT]GG
731 CCAAGG
XmnI 63 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsmFI BsrI BssKI BstNI BstYI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII