(Circular) (Six-base) MAPSORT of: pFN21AB7885.seq Check: 6410 from: 1 to: 6448 pFN21AB7885 6448 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4258 278 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3791 1937 Size: 1854 4594 Acc65I G'GTAC_C Cuts at: 3769 3769 Size: 6448 AclI AA'CG_TT Cuts at: 1563 4575 4948 1563 Size: 3012 373 3063 Fragments arranged by size: 3063 3012 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 3141 3207 4511 5895 6163 1632 Size: 519 990 66 1304 1384 268 1917 Fragments arranged by size: 1917 1384 1304 990 519 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6251 AflIII A'CryG_T Cuts at: 1203 3153 5347 6324 1203 Size: 1950 2194 977 1327 Fragments arranged by size: 2194 1950 1327 977 AgeI A'CCGG_T Cuts at: 5279 5279 Size: 6448 AhdI GACnn_n'nnGTC Cuts at: 3511 3553 5177 3511 Size: 42 1624 4782 Fragments arranged by size: 4782 1624 42 AleI CACnn'nnGTG Cuts at: 1166 2514 2621 3189 1166 Size: 1348 107 568 4425 Fragments arranged by size: 4425 1348 568 107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6448 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2411 2443 2411 Size: 32 6416 AlwNI CAG_nnn'CTG Cuts at: 2680 3097 3378 4191 5763 2680 Size: 417 281 813 1572 3365 Fragments arranged by size: 3365 1572 813 417 281 ApaLI G'TGCA_C Cuts at: 2042 2050 4505 5661 2042 Size: 8 2455 1156 2829 Fragments arranged by size: 2829 2455 1156 8 ApoI r'AATT_y Cuts at: 1493 2277 2710 3022 3281 3494 3757 3983 4135 Size: 784 433 312 259 213 263 226 152 Cuts at: 4135 6244 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 784 433 312 263 259 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 5002 160 Size: 872 3970 1606 Fragments arranged by size: 3970 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 AvaI C'yCGr_G Cuts at: 1870 1943 3773 1870 Size: 73 1830 4545 Fragments arranged by size: 4545 1830 73 BamHI G'GATC_C Cuts at: 2245 3778 4145 2245 Size: 1533 367 4548 Fragments arranged by size: 4548 1533 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3309 3769 5224 618 Size: 325 217 627 1522 460 1455 1842 Fragments arranged by size: 1842 1522 1455 627 460 325 217 BanII G_rGCy'C Cuts at: 1391 3767 1391 Size: 2376 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 2447 3464 3515 928 Size: 971 176 372 1017 51 3861 Fragments arranged by size: 3861 1017 971 372 176 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4639 4673 1903 Size: 34 2702 34 3678 Fragments arranged by size: 3678 2702 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4340 5550 4340 Size: 1210 5238 BclI T'GATC_A Cuts at: 1548 2227 1548 Size: 679 5769 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5059 136 Size: 107 122 71 631 605 3387 1525 Fragments arranged by size: 3387 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3113 6443 3113 Size: 3330 3118 BlpI GC'TnA_GC Cuts at: 3865 3865 Size: 6448 Bme1580I G_kGCm'C Cuts at: 1790 2046 2054 4509 5665 1790 Size: 256 8 2455 1156 2573 Fragments arranged by size: 2573 2455 1156 256 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3166 4179 5137 Size: 416 53 83 399 24 1749 1013 958 Cuts at: 5137 442 Size: 1753 Fragments arranged by size: 1753 1749 1013 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6448 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 6448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2801 3440 5108 1239 Size: 908 654 639 1668 2579 Fragments arranged by size: 2579 1668 908 654 639 Bpu10I CC'TnA_GC Cuts at: 2593 2593 Size: 6448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3423 4573 5438 5736 2927 Size: 496 1150 865 298 3639 Fragments arranged by size: 3639 1150 865 496 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5111 882 Size: 617 3612 2219 Fragments arranged by size: 3612 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4239 493 Size: 711 3035 2702 Fragments arranged by size: 3035 2702 711 BsaBI GATnn'nnATC Cuts at: 6442 6442 Size: 6448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4255 4637 5272 275 Size: 53 83 186 936 2722 382 635 1451 Fragments arranged by size: 2722 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4881 5279 5553 5700 6111 4881 Size: 398 274 147 411 5218 Fragments arranged by size: 5218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2411 2441 3126 3156 1178 Size: 30 165 30 1008 30 685 30 4470 Fragments arranged by size: 4470 1008 685 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2150 2948 2951 1186 Size: 922 42 798 3 4683 Fragments arranged by size: 4683 922 798 42 3 BseYI C'CCAG_C Cuts at: 2015 2055 5651 6147 2015 Size: 40 3596 496 2316 Fragments arranged by size: 3596 2316 496 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3213 1163 Size: 573 1477 4398 Fragments arranged by size: 4398 1477 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3898 4659 5687 1540 Size: 275 186 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2054 4509 4594 5665 2046 Size: 8 2455 85 1071 2829 Fragments arranged by size: 2829 2455 1071 85 8 BsmI GAATG_Cn' Cuts at: 3959 4052 3959 Size: 93 6355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2054 3767 4509 4594 5665 1391 Size: 399 256 8 1713 742 85 1071 2174 Fragments arranged by size: 2174 1713 1071 742 399 256 85 8 BspEI T'CCGG_A Cuts at: 6111 6111 Size: 6448 BspHI T'CATG_A Cuts at: 2632 4337 6067 2632 Size: 1705 1730 3013 Fragments arranged by size: 3013 1730 1705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3803 844 Size: 2959 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2911 4335 1394 Size: 429 1088 1424 3507 Fragments arranged by size: 3507 1424 1088 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4943 5117 65 Size: 1183 1821 1874 174 1396 Fragments arranged by size: 1874 1821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5092 5279 1700 Size: 110 3282 187 2869 Fragments arranged by size: 3282 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3319 96 Size: 3223 3225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6448 BssSI C'ACGA_G Cuts at: 4508 5520 4508 Size: 1012 5436 BstAPI GCAn_nnn'nTGC Cuts at: 794 3262 794 Size: 2468 3980 BstBI TT'CG_AA Cuts at: 2836 5252 2836 Size: 2416 4032 BstEII G'GTnAC_C Cuts at: 1184 2980 1184 Size: 1796 4652 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3681 2011 Size: 1670 4778 BstYI r'GATC_y Cuts at: 1972 2245 3006 3113 3374 3699 3778 4145 4530 Size: 273 761 107 261 325 79 367 385 Cuts at: 4530 4547 5999 6085 6097 6443 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 761 385 367 346 325 273 261 107 86 79 17 12 Bsu36I CC'TnA_GG Cuts at: 3636 3636 Size: 6448 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4957 Fragments arranged by size: 4957 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5709 Fragments arranged by size: 5709 683 56 BtsI GCAGTG_nn' Cuts at: 2264 3964 4758 4778 2264 Size: 1700 794 20 3934 Fragments arranged by size: 3934 1700 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6413 DraI TTT'AAA Cuts at: 3752 3752 Size: 6448 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5455 809 Size: 507 4139 1802 Fragments arranged by size: 4139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 3895 4784 Size: 54 1425 50 275 18 573 1492 889 Cuts at: 4784 6413 8 Size: 1629 43 Fragments arranged by size: 1629 1492 1425 889 573 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3895 1537 Size: 275 2083 4090 Fragments arranged by size: 4090 2083 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3117 4378 1353 Size: 774 990 1261 3423 Fragments arranged by size: 3423 1261 990 774 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5035 5409 5555 387 Size: 1064 138 3446 374 146 1280 Fragments arranged by size: 3446 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2151 2801 3141 3207 3440 4511 Size: 393 515 4 650 340 66 233 1071 Cuts at: 4511 5108 5895 6163 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1071 787 650 597 515 393 340 268 233 66 4 EcoNI CCTnn'n_nnAGG Cuts at: 3663 3663 Size: 6448 EcoO109I rG'GnC_Cy Cuts at: 2597 2740 2841 2597 Size: 143 101 6204 Fragments arranged by size: 6204 143 101 EcoRI G'AATT_C Cuts at: 2710 3757 4135 2710 Size: 1047 378 5023 Fragments arranged by size: 5023 1047 378 FspI TGC'GCA Cuts at: 4954 4954 Size: 6448 HaeII r_GCGC'y Cuts at: 1678 5595 1678 Size: 3917 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2846 2878 3359 Size: 32 135 32 658 32 584 32 481 Cuts at: 3359 3391 3497 3529 3539 3571 5089 5121 5163 Size: 32 106 32 10 32 1518 32 42 Cuts at: 5163 5195 1373 Size: 32 2626 Fragments arranged by size: 2626 1518 658 584 481 135 106 42 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3181 3792 4038 669 Size: 988 281 1243 611 246 3079 Fragments arranged by size: 3079 1243 988 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6448 HpaI GTT'AAC Cuts at: 4038 4038 Size: 6448 KpnI G_GTAC'C Cuts at: 3773 3773 Size: 6448 MfeI C'AATT_G Cuts at: 2118 4047 2118 Size: 1929 4519 MluI A'CGCG_T Cuts at: 6324 6324 Size: 6448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5562 5746 1305 Size: 4257 184 2007 Fragments arranged by size: 4257 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 10 Size: 54 1425 343 573 4053 Fragments arranged by size: 4053 1425 573 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2514 2621 3189 4406 4765 4924 Size: 648 843 505 107 568 1217 359 159 Cuts at: 4924 518 Size: 2042 Fragments arranged by size: 2042 1217 843 648 568 505 359 159 107 MspA1I CmG'CkG Cuts at: 1606 2038 3864 4541 5689 5934 1606 Size: 432 1826 677 1148 245 2120 Fragments arranged by size: 2120 1826 1148 677 432 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4957 Fragments arranged by size: 4957 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6448 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6448 NotI GC'GGCC_GC Cuts at: 3895 3895 Size: 6448 NspI r_CATG'y Cuts at: 1132 1229 3157 3806 5351 1132 Size: 97 1928 649 1545 2229 Fragments arranged by size: 2229 1928 1545 649 97 PasI CC'CwG_GG Cuts at: 1348 3330 1348 Size: 1982 4466 PciI A'CATG_T Cuts at: 3153 5347 3153 Size: 2194 4254 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6448 PfoI T'CCnGG_A Cuts at: 3009 3270 3009 Size: 261 6187 PmeI GTTT'AAAC Cuts at: 3752 3752 Size: 6448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2443 4517 4549 6030 6062 2411 Size: 32 2074 32 1481 32 2797 Fragments arranged by size: 2797 2074 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2740 2841 2740 Size: 101 6347 PshAI GACnn'nnGTC Cuts at: 1613 2846 3591 1613 Size: 1233 745 4470 Fragments arranged by size: 4470 1233 745 PsiI TTA'TAA Cuts at: 2207 4018 2207 Size: 1811 4637 PstI C_TGCA'G Cuts at: 830 2380 3800 830 Size: 1550 1420 3478 Fragments arranged by size: 3478 1550 1420 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6448 SalI G'TCGA_C Cuts at: 1936 3790 1936 Size: 1854 4594 SapI GCTCTTCn'nnn_ Cuts at: 3117 3117 Size: 6448 SbfI CC_TGCA'GG Cuts at: 3800 3800 Size: 6448 ScaI AGT'ACT Cuts at: 4696 6408 4696 Size: 1712 4736 SfcI C'TryA_G Cuts at: 826 1045 2376 3796 4931 5612 5803 826 Size: 219 1331 1420 1135 681 191 1471 Fragments arranged by size: 1471 1420 1331 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6448 SmaI CCC'GGG Cuts at: 1872 3775 1872 Size: 1903 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2906 3438 4552 5453 5715 820 Size: 197 926 963 532 1114 901 262 1553 Fragments arranged by size: 1553 1114 963 926 901 532 262 197 SnaBI TAC'GTA Cuts at: 493 4239 493 Size: 3746 2702 SpeI A'CTAG_T Cuts at: 152 2828 152 Size: 2676 3772 SphI G_CATG'C Cuts at: 1132 3806 1132 Size: 2674 3774 SspI AAT'ATT Cuts at: 5 52 4372 5 Size: 47 4320 2081 Fragments arranged by size: 4320 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6448 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 2736 3887 513 Size: 552 939 402 330 1151 3074 Fragments arranged by size: 3074 1151 939 552 402 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 4475 4492 4645 4830 5275 325 Size: 1255 489 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2627 3319 4694 Size: 274 80 33 51 1443 650 692 1375 Cuts at: 4694 6406 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1375 692 650 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6408 6440 6408 Size: 32 6416 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6448 XbaI T'CTAG_A Cuts at: 3784 3784 Size: 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6448 XmaI C'CCGG_G Cuts at: 1870 3773 1870 Size: 1903 4545 XmnI GAAnn'nnTTC Cuts at: 2072 3212 4577 2072 Size: 1140 1365 3943 Fragments arranged by size: 3943 1365 1140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4256 276 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI