Restriction map for
pFN21AB7885
(Linear) (Six-base) MAPSORT of: KIBB7885.seq Check: 9256 from: 1 to: 1743
KIBB7885.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1136 1202 1743
Size: 146 990 66 541
Fragments arranged by size:
990 541 146 66
AflIII A'CryG_T
Cuts at: 0 1148 1743
Size: 1148 595
AhdI GACnn_n'nnGTC
Cuts at: 0 1506 1548 1743
Size: 1506 42 195
Fragments arranged by size:
1506 195 42
AleI CACnn'nnGTG
Cuts at: 0 509 616 1184 1743
Size: 509 107 568 559
Fragments arranged by size:
568 559 509 107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 406 438 1743
Size: 406 32 1305
Fragments arranged by size:
1305 406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 675 1092 1373 1743
Size: 675 417 281 370
Fragments arranged by size:
675 417 370 281
ApaLI G'TGCA_C
Cuts at: 0 37 45 1743
Size: 37 8 1698
Fragments arranged by size:
1698 37 8
ApoI r'AATT_y
Cuts at: 0 272 705 1017 1276 1489 1743
Size: 272 433 312 259 213 254
Fragments arranged by size:
433 312 272 259 254 213
BamHI G'GATC_C
Cuts at: 0 240 1743
Size: 240 1503
BanI G'GyrC_C
Cuts at: 0 1304 1743
Size: 1304 439
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 442 1459 1510 1743
Size: 70 372 1017 51 233
Fragments arranged by size:
1017 372 233 70 51
BclI T'GATC_A
Cuts at: 0 222 1743
Size: 222 1521
BglII A'GATC_T
Cuts at: 0 1108 1743
Size: 1108 635
Bme1580I G_kGCm'C
Cuts at: 0 41 49 1743
Size: 41 8 1694
Fragments arranged by size:
1694 41 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1161 1743
Size: 1161 582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1257 1743
Size: 1257 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 796 1435 1743
Size: 142 654 639 308
Fragments arranged by size:
654 639 308 142
Bpu10I CC'TnA_GC
Cuts at: 0 588 1743
Size: 588 1155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1418 1743
Size: 922 496 325
Fragments arranged by size:
922 496 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 1121 1151 1743
Size: 406 30 685 30 592
Fragments arranged by size:
685 592 406 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 145 943 946 1743
Size: 103 42 798 3 797
Fragments arranged by size:
798 797 103 42 3
BseYI C'CCAG_C
Cuts at: 0 10 50 1743
Size: 10 40 1693
Fragments arranged by size:
1693 40 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1743
Size: 1208 535
BsiHKAI G_wGCw'C
Cuts at: 0 41 49 1743
Size: 41 8 1694
Fragments arranged by size:
1694 41 8
Bsp1286I G_dGCh'C
Cuts at: 0 41 49 1743
Size: 41 8 1694
Fragments arranged by size:
1694 41 8
BspHI T'CATG_A
Cuts at: 0 627 1743
Size: 627 1116
BsrBI CCG'CTC
Cuts at: 0 906 1743
Size: 906 837
BsrDI GCAATG_nn'
Cuts at: 0 1064 1743
Size: 1064 679
BsrGI T'GTAC_A
Cuts at: 0 1314 1743
Size: 1314 429
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1257 1743
Size: 1257 486
BstBI TT'CG_AA
Cuts at: 0 831 1743
Size: 831 912
BstEII G'GTnAC_C
Cuts at: 0 975 1743
Size: 975 768
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1676 1743
Size: 1676 67
BstYI r'GATC_y
Cuts at: 0 240 1001 1108 1369 1694 1743
Size: 240 761 107 261 325 49
Fragments arranged by size:
761 325 261 240 107 49
Bsu36I CC'TnA_GG
Cuts at: 0 1631 1743
Size: 1631 112
BtsI GCAGTG_nn'
Cuts at: 0 259 1743
Size: 259 1484
EaeI y'GGCC_r
Cuts at: 0 398 1743
Size: 398 1345
EarI CTCTTCn'nnn_
Cuts at: 0 122 1112 1743
Size: 122 990 631
Fragments arranged by size:
990 631 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 146 796 1136 1202 1435 1743
Size: 142 4 650 340 66 233 308
Fragments arranged by size:
650 340 308 233 142 66 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1658 1743
Size: 1658 85
EcoO109I rG'GnC_Cy
Cuts at: 0 592 735 836 1743
Size: 592 143 101 907
Fragments arranged by size:
907 592 143 101
EcoRI G'AATT_C
Cuts at: 0 705 1743
Size: 705 1038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 841 873 1354 1386 1492 1524
Size: 225 32 584 32 481 32 106 32
Cuts at: 1524 1534 1566 1743
Size: 10 32 177
Fragments arranged by size:
584 481 225 177 106 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 1176 1743
Size: 1176 567
MfeI C'AATT_G
Cuts at: 0 113 1743
Size: 113 1630
MscI TGG'CCA
Cuts at: 0 400 1743
Size: 400 1343
MslI CAynn'nnrTG
Cuts at: 0 509 616 1184 1743
Size: 509 107 568 559
Fragments arranged by size:
568 559 509 107
MspA1I CmG'CkG
Cuts at: 0 33 1743
Size: 33 1710
NspI r_CATG'y
Cuts at: 0 1152 1743
Size: 1152 591
PasI CC'CwG_GG
Cuts at: 0 1325 1743
Size: 1325 418
PciI A'CATG_T
Cuts at: 0 1148 1743
Size: 1148 595
PfoI T'CCnGG_A
Cuts at: 0 1004 1265 1743
Size: 1004 261 478
Fragments arranged by size:
1004 478 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 1743
Size: 406 32 1305
Fragments arranged by size:
1305 406 32
PpuMI rG'GwC_Cy
Cuts at: 0 735 836 1743
Size: 735 101 907
Fragments arranged by size:
907 735 101
PshAI GACnn'nnGTC
Cuts at: 0 841 1586 1743
Size: 841 745 157
Fragments arranged by size:
841 745 157
PsiI TTA'TAA
Cuts at: 0 202 1743
Size: 202 1541
PstI C_TGCA'G
Cuts at: 0 375 1743
Size: 375 1368
SapI GCTCTTCn'nnn_
Cuts at: 0 1112 1743
Size: 1112 631
SfcI C'TryA_G
Cuts at: 0 371 1743
Size: 371 1372
SmlI C'TyrA_G
Cuts at: 0 901 1433 1743
Size: 901 532 310
Fragments arranged by size:
901 532 310
SpeI A'CTAG_T
Cuts at: 0 823 1743
Size: 823 920
StyI C'CwwG_G
Cuts at: 0 401 731 1743
Size: 401 330 1012
Fragments arranged by size:
1012 401 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 1743
Size: 64 1679
TatI w'GTAC_w
Cuts at: 0 622 1314 1743
Size: 622 692 429
Fragments arranged by size:
692 622 429
XmnI GAAnn'nnTTC
Cuts at: 0 67 1207 1743
Size: 67 1140 536
Fragments arranged by size:
1140 536 67
Enzymes that do cut:
AcuI AflIII AhdI AleI AloI AlwNI ApaLI ApoI
BamHI BanI BbsI BclI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtsI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII MfeI MscI MslI MspA1I
NspI PasI PciI PfoI PpiI PpuMI PshAI PsiI
PstI SapI SfcI SmlI SpeI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrFI BssHII BssSI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI