Restriction map for
pFN21AE0175
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfcI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 475 AAATTC [GA]^AATT[CT]
AfaI 798 GTAC GT^AC
AhdI 734 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 475 AAATTC [GA]^AATT[CT]
AspEI 734 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4 GAGCTC G[AT]GC[AT]^C
AvaI 220 CCCGAG C^[CT]CG[GA]G
BbsI 865 GTCTTC GAAGAC(2/6)
BbvCI 695 CCTCAGC CCTCAGC(-5/-2)
BciVI 519 GGATAC GTATCC(6/5)
BglII 418 AGATCT A^GATCT
BpmI 311 CTGGAG CTGGAG(16/14)
BpuAI 865 GTCTTC GAAGAC(2/6)
BsaAI 109 TACGTA [CT]AC^GT[GA]
BseRI 493 GAGGAG GAGGAG(10/8)
BsgI 386 GTGCAG GTGCAG(16/14)
BsiHKAI 4 GAGCTC G[AT]GC[AT]^C
BsoBI 220 CCCGAG C^[CT]CG[GA]G
BsrBI 663 CCGCTC CCGCTC(-3/-3)
BsrI 301 ACTGG ACTGG(1/-1)
BstYI 418 AGATCT [GA]^GATC[CT]
CfoI 143 GCGC GCG^C
DraIII 196 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 163 GACACATCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 524 CCGTGG C^C[GA][CT]GG
EaeI 716 CGGCCA [CT]^GGCC[GA]
Eam1105I 734 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 21 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 142 GGCGCT [GA]GCGC^[CT]
HhaI 143 GCGC GCG^C
HinP1I 143 GCGC G^CGC
MaeII 110 ACGT A^CGT
MflI 418 AGATCT [GA]^GATC[CT]
MslI 459 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 186 GCATGT [GA]CATG^[CT]
PvuII 680 CAGCTG CAG^CTG
RsaI 798 GTAC GT^AC
SacI 4 GAGCTC GAGCT^C
SapI 537 GAAGAGC GCTCTTC(1/4)
SexAI 197 ACCTGGT A^CC[AT]GGT
SfaNI 345 GCATC GCATC(5/9)
SfiI 708 GGCCCCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 340 CTTGAG C^T[CT][GA]AG
SnaBI 109 TACGTA TAC^GTA
StuI 363 AGGCCT AGG^CCT
XcmI 839 CCACTGTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 418 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 133 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
830 CAGCACCTG
AvaII 87 GGTCC G^G[AT]CC
192 GGACC
BanI 529 GGTGCC G^G[CT][GA]CC
762 GGTGCC
BanII 4 GAGCTC G[GA]GC[CT]^C
331 GAGCCC
BglI 700 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
709 GCCCCCTCGGC
BsmAI 293 GAGAC GTCTC(1/5)
844 GTCTC
DpnII 419 GATC ^GATC
579 GATC
DraII 407 AGGCCCC [GA]G^G[ACGT]CC[CT]
690 AGGCCCC
EarI 271 GAAGAG CTCTTC(1/4)
537 GAAGAG
EcoO109I 407 AGGCCCC [GA]G^G[ACGT]CC[CT]
690 AGGCCCC
FauI 611 CCCGC CCCGC(4/6)
277 GCGGG
Ksp632I 271 GAAGAG CTCTTC(1/4)
537 GAAGAG
MaeIII 16 GTCAC ^GT[ACGT]AC
859 GTCAC
MboI 419 GATC ^GATC
579 GATC
MspA1I 120 CAGCGG C[AC]G^C[GT]G
680 CAGCTG
NdeII 419 GATC ^GATC
579 GATC
NlaIII 187 CATG CATG^
759 CATG
PflMI 132 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
195 CCACCTGGTGG
PleI 295 GACTC GAGTC(4/5)
857 GAGTC
Sau3AI 419 GATC ^GATC
579 GATC
Tsp45I 16 GTCAC ^GT[GC]AC
859 GTCAC
Van91I 132 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
195 CCACCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BspMI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MboII MnlI MvaI MwoI NlaIV
Sau96I ScrFI StyI TfiI TseI
Tsp509I TspRI