Restriction map for
pFN21AE0175
(Linear) (Six-base) MAPSORT of: KIEE0175.seq Check: 4567 from: 1 to: 903
KIEE0175.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 49 373 442 843 903
Size: 49 324 69 401 60
Fragments arranged by size:
401 324 69 60 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 556 855 903
Size: 290 266 299 48
Fragments arranged by size:
299 290 266 48
AhdI GACnn_n'nnGTC
Cuts at: 0 739 903
Size: 739 164
AlwNI CAG_nnn'CTG
Cuts at: 0 138 835 903
Size: 138 697 68
Fragments arranged by size:
697 138 68
ApoI r'AATT_y
Cuts at: 0 475 903
Size: 475 428
AvaI C'yCGr_G
Cuts at: 0 220 903
Size: 220 683
BanI G'GyrC_C
Cuts at: 0 529 762 903
Size: 529 233 141
Fragments arranged by size:
529 233 141
BanII G_rGCy'C
Cuts at: 0 8 335 903
Size: 8 327 568
Fragments arranged by size:
568 327 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 858 903
Size: 858 45
BbvCI CC'TCA_GC
Cuts at: 0 696 903
Size: 696 207
BciVI GTATCCnnnnn_n'
Cuts at: 0 513 903
Size: 513 390
BglI GCCn_nnn'nGGC
Cuts at: 0 706 715 903
Size: 706 9 188
Fragments arranged by size:
706 188 9
BglII A'GATC_T
Cuts at: 0 418 903
Size: 418 485
Bme1580I G_kGCm'C
Cuts at: 0 534 903
Size: 534 369
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 903
Size: 311 592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 903
Size: 332 571
Bpu10I CC'TnA_GC
Cuts at: 0 696 903
Size: 696 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 903
Size: 361 542
BsaAI yAC'GTr
Cuts at: 0 111 903
Size: 111 792
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 848 878 903
Size: 848 30 25
Fragments arranged by size:
848 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 903
Size: 508 395
BseYI C'CCAG_C
Cuts at: 0 316 334 445 606 903
Size: 316 18 111 161 297
Fragments arranged by size:
316 297 161 111 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 903
Size: 407 496
BsiHKAI G_wGCw'C
Cuts at: 0 8 903
Size: 8 895
Bsp1286I G_dGCh'C
Cuts at: 0 8 335 534 903
Size: 8 327 199 369
Fragments arranged by size:
369 327 199 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 373 442 843 903
Size: 49 324 69 401 60
Fragments arranged by size:
401 324 69 60 49
BsrBI CCG'CTC
Cuts at: 0 665 903
Size: 665 238
BstYI r'GATC_y
Cuts at: 0 418 903
Size: 418 485
BtgI C'CryG_G
Cuts at: 0 524 903
Size: 524 379
BtsI GCAGTG_nn'
Cuts at: 0 616 654 903
Size: 616 38 249
Fragments arranged by size:
616 249 38
DraIII CAC_nnn'GTG
Cuts at: 0 201 903
Size: 201 702
DrdI GACnn_nn'nnGTC
Cuts at: 0 169 903
Size: 169 734
EaeI y'GGCC_r
Cuts at: 0 716 903
Size: 716 187
EarI CTCTTCn'nnn_
Cuts at: 0 266 532 903
Size: 266 266 371
Fragments arranged by size:
371 266 266
EcoICRI GAG'CTC
Cuts at: 0 6 903
Size: 6 897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 332 556 855 903
Size: 290 42 224 299 48
Fragments arranged by size:
299 290 224 48 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 903
Size: 25 878
EcoO109I rG'GnC_Cy
Cuts at: 0 408 691 903
Size: 408 283 212
Fragments arranged by size:
408 283 212
HaeII r_GCGC'y
Cuts at: 0 146 903
Size: 146 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 848 880 903
Size: 725 32 91 32 23
Fragments arranged by size:
725 91 32 32 23
MslI CAynn'nnrTG
Cuts at: 0 463 903
Size: 463 440
MspA1I CmG'CkG
Cuts at: 0 122 682 903
Size: 122 560 221
Fragments arranged by size:
560 221 122
NspI r_CATG'y
Cuts at: 0 190 903
Size: 190 713
PasI CC'CwG_GG
Cuts at: 0 673 903
Size: 673 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 201 903
Size: 138 63 702
Fragments arranged by size:
702 138 63
PvuII CAG'CTG
Cuts at: 0 682 903
Size: 682 221
SacI G_AGCT'C
Cuts at: 0 8 903
Size: 8 895
SapI GCTCTTCn'nnn_
Cuts at: 0 532 903
Size: 532 371
SexAI A'CCwGG_T
Cuts at: 0 197 903
Size: 197 706
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 715 903
Size: 715 188
SmlI C'TyrA_G
Cuts at: 0 340 903
Size: 340 563
SnaBI TAC'GTA
Cuts at: 0 111 903
Size: 111 792
StuI AGG'CCT
Cuts at: 0 365 903
Size: 365 538
StyI C'CwwG_G
Cuts at: 0 83 210 360 704 903
Size: 83 127 150 344 199
Fragments arranged by size:
344 199 150 127 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 517 744 775 903
Size: 517 227 31 128
Fragments arranged by size:
517 227 128 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 903
Size: 846 57
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApoI AvaI BanI BanII
BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BstYI BtgI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
MslI MspA1I NspI PasI PflMI PvuII SacI SapI
SexAI SfiI SmlI SnaBI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BlpI
BmgBI BmtI BplI BsaI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI