(Circular) (Six-base) MAPSORT of: pFN21AE0207.seq Check: 8557 from: 1 to: 5536 pFN21AE0207 5536 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2261 2261 Size: 5536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3599 4983 5251 1632 Size: 1967 1384 268 1917 Fragments arranged by size: 1967 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 2627 4435 5412 1203 Size: 1424 1808 977 1327 Fragments arranged by size: 1808 1424 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 2678 3279 4851 2678 Size: 601 1572 3363 Fragments arranged by size: 3363 1572 601 ApaI G_GGCC'C Cuts at: 2085 2106 2085 Size: 21 5515 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2845 3071 3223 5332 1493 Size: 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2162 2861 1870 Size: 73 162 57 699 4545 Fragments arranged by size: 4545 699 162 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2614 2647 2614 Size: 33 5503 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2857 4312 618 Size: 325 217 627 1070 1455 1842 Fragments arranged by size: 1842 1455 1070 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2085 2106 2788 2855 1391 Size: 655 39 21 682 67 4072 Fragments arranged by size: 4072 682 655 67 39 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 2247 1548 Size: 699 4837 BfrBI ATG'CAT Cuts at: 2296 2296 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4147 136 Size: 107 122 71 631 605 337 2138 1525 Fragments arranged by size: 2138 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2085 2106 2242 3597 4753 1790 Size: 295 21 136 1355 1156 2573 Fragments arranged by size: 2573 1355 1156 295 136 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4196 1239 Size: 2957 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3661 4526 4824 3661 Size: 865 298 4373 Fragments arranged by size: 4373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2630 3327 493 Size: 711 1426 697 2702 Fragments arranged by size: 2702 1426 711 697 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5536 BseYI C'CCAG_C Cuts at: 2099 4739 5235 2099 Size: 2640 496 2400 Fragments arranged by size: 2640 2400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4963 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3597 3682 4753 3597 Size: 85 1071 4380 Fragments arranged by size: 4380 1071 85 BsiWI C'GTAC_G Cuts at: 2136 2136 Size: 5536 BsmI GAATG_Cn' Cuts at: 2353 3047 3140 2353 Size: 694 93 4749 Fragments arranged by size: 4749 694 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2085 2106 2242 2788 2855 3597 Size: 399 256 39 21 136 546 67 742 Cuts at: 3597 3682 4753 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 256 136 85 67 39 21 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 2891 844 Size: 1417 630 3489 Fragments arranged by size: 3489 1417 630 BsrBI CCG'CTC Cuts at: 1394 1823 3423 1394 Size: 429 1600 3507 Fragments arranged by size: 3507 1600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2332 2530 4031 4205 65 Size: 1183 1084 198 1501 174 1396 Fragments arranged by size: 1501 1396 1183 1084 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4180 4367 1700 Size: 110 2370 187 2869 Fragments arranged by size: 2869 2370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 2678 794 Size: 1884 3652 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstYI r'GATC_y Cuts at: 1972 2866 3233 3618 3635 5087 5173 5185 5531 Size: 894 367 385 17 1452 86 12 346 Cuts at: 5531 1972 Size: 1977 Fragments arranged by size: 1977 1452 894 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2505 513 Size: 552 939 501 3544 Fragments arranged by size: 3544 939 552 501 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2590 2676 3052 3846 3866 2590 Size: 86 376 794 20 4260 Fragments arranged by size: 4260 794 376 86 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2671 2706 529 Size: 35 2107 35 3359 Fragments arranged by size: 3359 2107 35 35 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2487 4543 809 Size: 507 1171 2056 1802 Fragments arranged by size: 2056 1802 1171 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2983 3872 5501 Size: 54 1425 50 275 18 1153 889 1629 Cuts at: 5501 8 Size: 43 Fragments arranged by size: 1629 1425 1153 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4123 4497 4643 387 Size: 1064 138 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3599 4196 4983 5251 1239 Size: 393 1967 597 787 268 1524 Fragments arranged by size: 1967 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2081 2194 2465 2081 Size: 113 271 5152 Fragments arranged by size: 5152 271 113 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 4683 1678 Size: 3005 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373 Size: 32 135 32 2605 32 42 32 2626 Fragments arranged by size: 2626 2605 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3714 Fragments arranged by size: 3714 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2301 3494 3853 4012 518 Size: 648 1113 22 1193 359 159 2042 Fragments arranged by size: 2042 1193 1113 648 359 159 22 MspA1I CmG'CkG Cuts at: 1606 2032 2952 3629 4777 5022 1606 Size: 426 920 677 1148 245 2120 Fragments arranged by size: 2120 1148 920 677 426 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2505 513 Size: 552 939 501 3544 Fragments arranged by size: 3544 939 552 501 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NsiI A_TGCA'T Cuts at: 2298 2298 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2306 2430 2714 2894 4439 1132 Size: 97 1077 124 284 180 1545 2229 Fragments arranged by size: 2229 1545 1077 284 180 124 97 PasI CC'CwG_GG Cuts at: 1348 2357 1348 Size: 1009 4527 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 4138 PfoI T'CCnGG_A Cuts at: 2476 2476 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2194 Size: 5536 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PspOMI G'GGCC_C Cuts at: 2081 2102 2081 Size: 21 5515 PstI C_TGCA'G Cuts at: 830 2597 2888 830 Size: 1767 291 3478 Fragments arranged by size: 3478 1767 291 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2593 2884 4019 4700 4891 826 Size: 219 1548 291 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 681 291 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2107 2863 1872 Size: 235 756 4545 Fragments arranged by size: 4545 756 235 SmlI C'TyrA_G Cuts at: 820 1017 1943 3640 4541 4803 820 Size: 197 926 1697 901 262 1553 Fragments arranged by size: 1697 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2430 2714 2894 1132 Size: 1298 284 180 3774 Fragments arranged by size: 3774 1298 284 180 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 2115 1517 Size: 598 4938 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 2975 513 Size: 552 939 501 470 3074 Fragments arranged by size: 3074 939 552 501 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 3563 3580 3733 3918 4363 325 Size: 1255 918 1065 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1065 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3782 5494 96 Size: 274 80 33 51 1443 1805 1712 138 Fragments arranged by size: 1805 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2105 2861 1870 Size: 235 756 4545 Fragments arranged by size: 4545 756 235 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI