(Circular) (Six-base) MAPSORT of: pFN21AE0103.seq Check: 3871 from: 1 to: 5905 pFN21AE0103 5905 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2407 2407 Size: 5905 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 3248 1937 Size: 667 644 4594 Fragments arranged by size: 4594 667 644 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3968 5352 5620 1632 Size: 2336 1384 268 1917 Fragments arranged by size: 2336 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2044 4804 5781 1203 Size: 841 2760 977 1327 Fragments arranged by size: 2760 1327 977 841 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 3002 1166 Size: 1836 4069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 3648 5220 3648 Size: 1572 4333 ApaI G_GGCC'C Cuts at: 2774 3133 2774 Size: 359 5546 ApaLI G'TGCA_C Cuts at: 2270 3962 5118 2270 Size: 1692 1156 3057 Fragments arranged by size: 3057 1692 1156 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2101 3230 1870 Size: 73 158 1129 4545 Fragments arranged by size: 4545 1129 158 73 BamHI G'GATC_C Cuts at: 2988 3235 3602 2988 Size: 247 367 5291 Fragments arranged by size: 5291 367 247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 3184 3226 4681 618 Size: 325 217 627 495 902 42 1455 1842 Fragments arranged by size: 1842 1455 902 627 495 325 217 42 BanII G_rGCy'C Cuts at: 1391 2124 2208 2774 3133 3224 1391 Size: 733 84 566 359 91 4072 Fragments arranged by size: 4072 733 566 359 91 84 BbeI G_GCGC'C Cuts at: 2286 2286 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2186 2930 928 Size: 971 287 744 3903 Fragments arranged by size: 3903 971 744 287 BbvCI CC'TCA_GC Cuts at: 2199 2199 Size: 5905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2467 2501 4096 4130 1903 Size: 34 530 34 1595 34 3678 Fragments arranged by size: 3678 1595 530 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2712 2839 3797 5007 2712 Size: 127 958 1210 3610 Fragments arranged by size: 3610 1210 958 127 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3149 4516 136 Size: 107 122 71 631 605 1477 1367 1525 Fragments arranged by size: 1525 1477 1367 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 2090 3322 2090 Size: 1232 4673 Bme1580I G_kGCm'C Cuts at: 1790 2274 2774 3133 3162 3966 5122 1790 Size: 484 500 359 29 804 1156 2573 Fragments arranged by size: 2573 1156 804 500 484 359 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2508 3636 4594 Size: 416 53 83 399 24 1091 1128 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1128 1091 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2657 4565 1239 Size: 1418 1908 2579 Fragments arranged by size: 2579 1908 1418 Bpu10I CC'TnA_GC Cuts at: 2199 2621 2199 Size: 422 5483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 3021 4568 882 Size: 617 591 931 1547 2219 Fragments arranged by size: 2219 1547 931 617 591 BsaAI yAC'GTr Cuts at: 493 1204 2359 2782 3696 493 Size: 711 1155 423 914 2702 Fragments arranged by size: 2702 1155 914 711 423 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 3712 4094 4729 Size: 53 83 186 936 750 1429 382 635 Cuts at: 4729 275 Size: 1451 Fragments arranged by size: 1451 1429 936 750 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2248 4338 4736 5010 5157 5568 2248 Size: 2090 398 274 147 411 2585 Fragments arranged by size: 2585 2090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 1178 Size: 30 165 30 1017 30 4633 Fragments arranged by size: 4633 1017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2900 3125 1186 Size: 1216 498 225 3966 Fragments arranged by size: 3966 1216 498 225 BseYI C'CCAG_C Cuts at: 2067 2773 5108 5604 2067 Size: 706 2335 496 2368 Fragments arranged by size: 2368 2335 706 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2846 1163 Size: 573 1110 4222 Fragments arranged by size: 4222 1110 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2491 3355 4116 5144 1540 Size: 275 186 490 864 761 1028 2301 Fragments arranged by size: 2301 1028 864 761 490 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 3966 4051 5122 2274 Size: 1692 85 1071 3057 Fragments arranged by size: 3057 1692 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 BsmBI CGTCTCn'nnnn_ Cuts at: 2423 2423 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2208 2274 2774 3133 3162 3224 Size: 399 334 84 66 500 359 29 62 Cuts at: 3224 3966 4051 5122 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 359 334 85 84 66 62 29 BspEI T'CCGG_A Cuts at: 2248 5568 2248 Size: 3320 2585 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 2559 3260 844 Size: 1563 152 701 3489 Fragments arranged by size: 3489 1563 701 152 BsrBI CCG'CTC Cuts at: 1394 1823 2751 3792 1394 Size: 429 928 1041 3507 Fragments arranged by size: 3507 1041 928 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3138 4400 4574 65 Size: 1183 1890 1262 174 1396 Fragments arranged by size: 1890 1396 1262 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3143 4549 4736 1700 Size: 110 1333 1406 187 2869 Fragments arranged by size: 2869 1406 1333 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 2810 1184 Size: 1626 4279 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2527 2965 3199 2521 Size: 6 438 234 5227 Fragments arranged by size: 5227 438 234 6 BstYI r'GATC_y Cuts at: 1972 2988 3235 3602 3987 4004 5456 5542 5554 Size: 1016 247 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 385 367 346 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2520 2665 2866 3093 513 Size: 552 939 516 145 201 227 3325 Fragments arranged by size: 3325 939 552 516 227 201 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2769 487 Size: 683 56 1543 3623 Fragments arranged by size: 3623 1543 683 56 BtsI GCAGTG_nn' Cuts at: 2532 3421 4215 4235 2532 Size: 889 794 20 4202 Fragments arranged by size: 4202 889 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DraIII CAC_nnn'GTG Cuts at: 2242 2242 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2723 2819 4912 809 Size: 507 1407 96 2093 1802 Fragments arranged by size: 2093 1802 1407 507 96 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2500 2955 Size: 54 1425 50 275 18 177 493 455 Cuts at: 2955 3069 3352 4241 5870 8 Size: 114 283 889 1629 43 Fragments arranged by size: 1629 1425 889 493 455 283 275 177 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3116 3835 1353 Size: 1763 719 3423 Fragments arranged by size: 3423 1763 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2657 3968 4565 5352 5620 1239 Size: 393 1025 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 1025 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2641 2932 3130 2641 Size: 291 198 5416 Fragments arranged by size: 5416 291 198 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2286 5052 1678 Size: 608 2766 2531 Fragments arranged by size: 2766 2531 608 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 3018 3050 4546 Size: 32 135 32 459 32 955 32 1496 Cuts at: 4546 4578 4620 4652 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1496 955 459 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2605 3037 3249 3495 669 Size: 988 281 667 432 212 246 3079 Fragments arranged by size: 3079 988 667 432 281 246 212 HindIII A'AGCT_T Cuts at: 748 2300 748 Size: 1552 4353 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2282 2282 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 5019 5203 1305 Size: 1875 1839 184 2007 Fragments arranged by size: 2007 1875 1839 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2502 2957 3071 10 Size: 54 1425 343 670 455 114 2844 Fragments arranged by size: 2844 1425 670 455 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2963 3002 3863 4222 4381 518 Size: 648 1359 438 39 861 359 159 2042 Fragments arranged by size: 2042 1359 861 648 438 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2806 2945 3321 3998 5146 5391 1606 Size: 1200 139 376 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 376 245 139 NaeI GCC'GGC Cuts at: 3145 3145 Size: 5905 NarI GG'CG_CC Cuts at: 2283 2283 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2004 2520 513 Size: 552 939 516 3898 Fragments arranged by size: 3898 939 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 3143 3143 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2048 3263 4808 1132 Size: 97 819 1215 1545 2229 Fragments arranged by size: 2229 1545 1215 819 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 2044 4804 2044 Size: 2760 3145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 2321 1108 Size: 1134 79 4692 Fragments arranged by size: 4692 1134 79 PfoI T'CCnGG_A Cuts at: 2079 2079 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PmlI CAC'GTG Cuts at: 2359 2782 2359 Size: 423 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 2641 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2770 3129 2770 Size: 359 5546 PstI C_TGCA'G Cuts at: 830 3257 830 Size: 2427 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2945 2945 Size: 5905 SalI G'TCGA_C Cuts at: 1936 2603 3247 1936 Size: 667 644 4594 Fragments arranged by size: 4594 667 644 SapI GCTCTTCn'nnn_ Cuts at: 3116 3116 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SexAI A'CCwGG_T Cuts at: 3030 3030 Size: 5905 SfcI C'TryA_G Cuts at: 826 1045 3253 4388 5069 5260 826 Size: 219 2208 1135 681 191 1471 Fragments arranged by size: 2208 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2284 2284 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 4833 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 3000 3344 513 Size: 552 939 516 480 344 3074 Fragments arranged by size: 3074 939 552 516 480 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2505 3932 3949 4102 4287 4732 325 Size: 1255 925 1427 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 925 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 4151 5863 Size: 274 80 33 51 1443 423 1751 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1751 1712 1443 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2253 2285 5865 5897 2253 Size: 32 3580 32 2261 Fragments arranged by size: 3580 2261 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2600 3032 1316 Size: 1284 432 4189 Fragments arranged by size: 4189 1284 432 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI