Restriction map for
pFN21AB4879
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BalI BanI BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AvrII 1236 CCTAGG C^CTAGG
BamHI 1510 GGATCC G^GATCC
BanII 1825 GAGCCC G[GA]GC[CT]^C
BbvCI 97 CCTCAGC CCTCAGC(-5/-2)
BcnI 1619 CCCGG CC^[GC]GG
BlnI 1236 CCTAGG C^CTAGG
BlpI 1816 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1816 GCTGAGC GC^T[ACGT]AGC
BsaAI 2279 CACGTA [CT]AC^GT[GA]
BsaBI 1463 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 408 ACCGGA [AT]^CCGG[AT]
BsmI 1164 GAATGC GAATGC(1/-1)
Bsp1407I 2340 TGTACA T^GTACA
BspLU11I 2378 ACATGT A^CATGT
BsrBI 211 GAGCGG CCGCTC(-3/-3)
BsrGI 2340 TGTACA T^GTACA
CelII 1816 GCTGAGC GC^T[ACGT]AGC
CfoI 1504 GCGC GCG^C
DrdI 2176 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1530 TGGCCG [CT]^GGCC[GA]
EcoRV 1228 GATATC GAT^ATC
HhaI 1504 GCGC GCG^C
HinP1I 1504 GCGC G^CGC
HincII 885 GTTAAC GT[CT]^[GA]AC
HindII 885 GTTAAC GT[CT]^[GA]AC
HpaI 885 GTTAAC GTT^AAC
MamI 1463 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 392 CAATTG C^AATTG
MslI 2379 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 392 CAATTG C^AATTG
NciI 1619 CCCGG CC^[GC]GG
NcoI 1032 CCATGG C^CATGG
PflMI 1032 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 1472 CTACAG C^T[GA][CT]AG
SspBI 2340 TGTACA T^GTACA
StuI 1576 AGGCCT AGG^CCT
Van91I 1032 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AatII 947 GACGTC GACGT^C
2098 GACGTC
AspHI 534 GTGCTC G[AT]GC[AT]^C
1562 GTGCTC
BclI 2300 TGATCA T^GATCA
2328 TGATCA
BpmI 1133 CTCCAG CTGGAG(16/14)
1358 CTGGAG
BseRI 249 CTCCTC GAGGAG(10/8)
1864 GAGGAG
BsgI 1679 CTGCAC GTGCAG(16/14)
455 GTGCAG
BsiHKAI 534 GTGCTC G[AT]GC[AT]^C
1562 GTGCTC
DraI 381 TTTAAA TTT^AAA
1057 TTTAAA
DsaI 1032 CCATGG C^C[GA][CT]GG
1933 CCACGG
FbaI 2300 TGATCA T^GATCA
2328 TGATCA
HgaI 1615 GCGTC GACGC(5/10)
1718 GCGTC
HindIII 161 AAGCTT A^AGCTT
593 AAGCTT
MspA1I 125 CAGCTG C[AC]G^C[GT]G
1640 CAGCTG
PpuMI 2019 AGGACCT [GA]G^G[AT]CC[CT]
2333 AGGTCCT
PshAI 1657 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1852 GACAAGAGTC
PvuII 125 CAGCTG CAG^CTG
1640 CAGCTG
SmlI 1159 CTTGAG C^T[CT][GA]AG
1538 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaHI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII