(Circular) (Six-base) MAPSORT of: pFN21AB4879.seq Check: 3238 from: 1 to: 7135 pFN21AB4879 7135 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3697 3697 Size: 7135 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2956 4107 4945 278 Size: 53 83 186 936 1420 1151 838 2468 Fragments arranged by size: 2468 1420 1151 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 4478 1937 Size: 2541 4594 Acc65I G'GTAC_C Cuts at: 4456 4456 Size: 7135 AclI AA'CG_TT Cuts at: 1563 5262 5635 1563 Size: 3699 373 3063 Fragments arranged by size: 3699 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2909 3338 3807 5198 6582 6850 1632 Size: 522 755 429 469 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 755 522 469 429 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6938 AflIII A'CryG_T Cuts at: 1203 3456 4283 4383 6034 7011 1203 Size: 2253 827 100 1651 977 1327 Fragments arranged by size: 2253 1651 1327 977 827 100 AgeI A'CCGG_T Cuts at: 5966 5966 Size: 7135 AhdI GACnn_n'nnGTC Cuts at: 5864 5864 Size: 7135 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3582 1184 Size: 2398 4737 AlwNI CAG_nnn'CTG Cuts at: 2132 2161 2408 3647 4878 6450 2132 Size: 29 247 1239 1231 1572 2817 Fragments arranged by size: 2817 1572 1239 1231 247 29 ApaLI G'TGCA_C Cuts at: 5192 6348 5192 Size: 1156 5979 ApoI r'AATT_y Cuts at: 1493 2041 2354 2845 3500 3521 4444 4670 4822 Size: 548 313 491 655 21 923 226 152 Cuts at: 4822 6931 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 923 655 548 491 313 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 5689 160 Size: 872 4657 1606 Fragments arranged by size: 4657 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 AvaI C'yCGr_G Cuts at: 1870 1943 4460 1870 Size: 73 2517 4545 Fragments arranged by size: 4545 2517 73 AvrII C'CTAG_G Cuts at: 3241 3241 Size: 7135 BamHI G'GATC_C Cuts at: 3515 4465 4832 3515 Size: 950 367 5818 Fragments arranged by size: 5818 950 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4456 5911 618 Size: 325 217 627 2669 1455 1842 Fragments arranged by size: 2669 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3834 4454 1391 Size: 2443 620 4072 Fragments arranged by size: 4072 2443 620 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2754 2904 2915 3807 4046 928 Size: 971 303 552 150 11 892 239 4017 Fragments arranged by size: 4017 971 892 552 303 239 150 11 BbvCI CC'TCA_GC Cuts at: 2103 2103 Size: 7135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5326 5360 1903 Size: 34 3389 34 3678 Fragments arranged by size: 3678 3389 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5027 6237 5027 Size: 1210 5925 BclI T'GATC_A Cuts at: 1548 4305 4333 1548 Size: 2757 28 4350 Fragments arranged by size: 4350 2757 28 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5746 136 Size: 107 122 71 631 605 4074 1525 Fragments arranged by size: 4074 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7130 7130 Size: 7135 BlpI GC'TnA_GC Cuts at: 3822 4552 3822 Size: 730 6405 Bme1580I G_kGCm'C Cuts at: 1790 5196 6352 1790 Size: 3406 1156 2573 Fragments arranged by size: 3406 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3023 3402 4866 Size: 416 53 83 399 24 1606 379 1464 Cuts at: 4866 5824 442 Size: 958 1753 Fragments arranged by size: 1753 1606 1464 958 416 399 379 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3123 3384 5795 1239 Size: 1884 261 2411 2579 Fragments arranged by size: 2579 2411 1884 261 Bpu10I CC'TnA_GC Cuts at: 2103 2478 2694 2977 3144 3813 2103 Size: 375 216 283 167 669 5425 Fragments arranged by size: 5425 669 375 283 216 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 3528 5260 6125 6423 3185 Size: 343 1732 865 298 3897 Fragments arranged by size: 3897 1732 865 343 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4118 4214 4409 5798 882 Size: 617 2619 96 195 1389 2219 Fragments arranged by size: 2619 2219 1389 617 195 96 BsaAI yAC'GTr Cuts at: 493 1204 4286 4926 493 Size: 711 3082 640 2702 Fragments arranged by size: 3082 2702 711 640 BsaBI GATnn'nnATC Cuts at: 3472 7129 3472 Size: 3657 3478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2953 3620 3723 4104 Size: 53 83 186 936 1420 667 103 381 Cuts at: 4104 4942 5324 5959 275 Size: 838 382 635 1451 Fragments arranged by size: 1451 1420 936 838 667 635 382 381 186 103 83 53 BsaWI w'CCGG_w Cuts at: 2413 5568 5966 6240 6387 6798 2413 Size: 3155 398 274 147 411 2750 Fragments arranged by size: 3155 2750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6910 Fragments arranged by size: 6910 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 3884 1186 Size: 1059 1639 4437 Fragments arranged by size: 4437 1639 1059 BseYI C'CCAG_C Cuts at: 2881 6338 6834 2881 Size: 3457 496 3182 Fragments arranged by size: 3457 3182 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2481 3669 1163 Size: 573 745 1188 4629 Fragments arranged by size: 4629 1188 745 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4585 5346 6374 1540 Size: 275 186 2584 761 1028 2301 Fragments arranged by size: 2584 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2543 3571 5196 5281 6352 2543 Size: 1028 1625 85 1071 3326 Fragments arranged by size: 3326 1625 1071 1028 85 BsmI GAATG_Cn' Cuts at: 3175 4646 4739 3175 Size: 1471 93 5571 Fragments arranged by size: 5571 1471 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2543 3571 3834 4454 5196 5281 6352 Size: 399 753 1028 263 620 742 85 1071 Cuts at: 6352 1391 Size: 2174 Fragments arranged by size: 2174 1071 1028 753 742 620 399 263 85 BspEI T'CCGG_A Cuts at: 6798 6798 Size: 7135 BspHI T'CATG_A Cuts at: 5024 6754 5024 Size: 1730 5405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2815 3117 3697 4490 844 Size: 1971 302 580 793 3489 Fragments arranged by size: 3489 1971 793 580 302 BsrBI CCG'CTC Cuts at: 1394 1823 2218 5022 1394 Size: 429 395 2804 3507 Fragments arranged by size: 3507 2804 429 395 BsrDI GCAATG_nn' Cuts at: 65 1248 5630 5804 65 Size: 1183 4382 174 1396 Fragments arranged by size: 4382 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5779 5966 1700 Size: 110 3969 187 2869 Fragments arranged by size: 3969 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4345 96 Size: 4249 2886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7135 BssSI C'ACGA_G Cuts at: 5195 6207 5195 Size: 1012 6123 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7135 BstBI TT'CG_AA Cuts at: 5939 5939 Size: 7135 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7135 BstYI r'GATC_y Cuts at: 1972 2440 2497 3515 4465 4832 5217 5234 6686 Size: 468 57 1018 950 367 385 17 1452 Cuts at: 6686 6772 6784 7130 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1018 950 468 385 367 346 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 3037 3938 513 Size: 552 1972 901 3710 Fragments arranged by size: 3710 1972 901 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6396 Fragments arranged by size: 6396 683 56 BtsI GCAGTG_nn' Cuts at: 2464 2635 4651 5445 5465 2464 Size: 171 2016 794 20 4134 Fragments arranged by size: 4134 2016 794 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7135 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7100 DraI TTT'AAA Cuts at: 2388 3064 4439 2388 Size: 676 1375 5084 Fragments arranged by size: 5084 1375 676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4187 6142 809 Size: 507 2871 1955 1802 Fragments arranged by size: 2871 1955 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4582 5471 Size: 54 1425 50 275 18 1705 1047 889 Cuts at: 5471 7100 8 Size: 1629 43 Fragments arranged by size: 1705 1629 1425 1047 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4582 1537 Size: 275 2770 4090 Fragments arranged by size: 4090 2770 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2058 2370 2529 3090 5065 1353 Size: 659 46 312 159 561 1975 3423 Fragments arranged by size: 3423 1975 659 561 312 159 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2794 5722 6096 6242 387 Size: 1064 138 1205 2928 374 146 1280 Fragments arranged by size: 2928 1280 1205 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2909 3123 3338 3384 3807 5198 Size: 393 522 755 214 215 46 423 1391 Cuts at: 5198 5795 6582 6850 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1391 787 755 597 522 423 393 268 215 214 46 EcoO109I rG'GnC_Cy Cuts at: 2392 4025 4339 2392 Size: 1633 314 5188 Fragments arranged by size: 5188 1633 314 EcoRI G'AATT_C Cuts at: 4444 4822 4444 Size: 378 6757 EcoRV GAT'ATC Cuts at: 3235 3235 Size: 7135 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2726 2726 Size: 7135 FspI TGC'GCA Cuts at: 5641 5641 Size: 7135 HaeII r_GCGC'y Cuts at: 1678 6282 1678 Size: 4604 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 3127 3159 3219 Size: 32 135 32 635 32 888 32 60 Cuts at: 3219 3251 3532 3564 5776 5808 5850 5882 1373 Size: 32 281 32 2212 32 42 32 2626 Fragments arranged by size: 2626 2212 888 635 281 135 60 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 4479 4725 669 Size: 988 281 954 1587 246 3079 Fragments arranged by size: 3079 1587 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 2166 2598 748 Size: 1418 432 5285 Fragments arranged by size: 5285 1418 432 HpaI GTT'AAC Cuts at: 2892 4725 2892 Size: 1833 5302 KpnI G_GTAC'C Cuts at: 4460 4460 Size: 7135 MfeI C'AATT_G Cuts at: 2397 4734 2397 Size: 2337 4798 MluI A'CGCG_T Cuts at: 7011 7011 Size: 7135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 6249 6433 1305 Size: 1329 3615 184 2007 Fragments arranged by size: 3615 2007 1329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5313 Fragments arranged by size: 5313 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4388 5093 5452 5611 518 Size: 648 3222 705 359 159 2042 Fragments arranged by size: 3222 2042 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2132 3647 4551 5228 6376 6621 1606 Size: 526 1515 904 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 904 677 526 245 NcoI C'CATG_G Cuts at: 513 1065 3037 513 Size: 552 1972 4611 Fragments arranged by size: 4611 1972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7135 NotI GC'GGCC_GC Cuts at: 4582 4582 Size: 7135 NspI r_CATG'y Cuts at: 1132 1229 2635 2693 4387 4493 6038 1132 Size: 97 1406 58 1694 106 1545 2229 Fragments arranged by size: 2229 1694 1545 1406 106 97 58 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7135 PciI A'CATG_T Cuts at: 4383 6034 4383 Size: 1651 5484 PflMI CCAn_nnn'nTGG Cuts at: 1108 3043 1108 Size: 1935 5200 PfoI T'CCnGG_A Cuts at: 3623 3623 Size: 7135 PmeI GTTT'AAAC Cuts at: 4439 4439 Size: 7135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2403 2435 5204 5236 6717 6749 2403 Size: 32 2769 32 1481 32 2789 Fragments arranged by size: 2789 2769 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4025 4339 4025 Size: 314 6821 PshAI GACnn'nnGTC Cuts at: 1613 3666 3861 1613 Size: 2053 195 4887 Fragments arranged by size: 4887 2053 195 PsiI TTA'TAA Cuts at: 3330 4705 3330 Size: 1375 5760 PstI C_TGCA'G Cuts at: 830 4487 830 Size: 3657 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7135 PvuII CAG'CTG Cuts at: 2132 3647 2132 Size: 1515 5620 SalI G'TCGA_C Cuts at: 1936 4477 1936 Size: 2541 4594 SbfI CC_TGCA'GG Cuts at: 4487 4487 Size: 7135 ScaI AGT'ACT Cuts at: 5383 7095 5383 Size: 1712 5423 SfcI C'TryA_G Cuts at: 826 1045 3477 4483 5618 6299 6490 826 Size: 219 2432 1006 1135 681 191 1471 Fragments arranged by size: 2432 1471 1135 1006 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7135 SmaI CCC'GGG Cuts at: 1872 4462 1872 Size: 2590 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3164 3543 5239 6140 6402 820 Size: 197 926 1221 379 1696 901 262 1553 Fragments arranged by size: 1696 1553 1221 926 901 379 262 197 SnaBI TAC'GTA Cuts at: 493 4926 493 Size: 4433 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7135 SphI G_CATG'C Cuts at: 1132 4493 1132 Size: 3361 3774 SspI AAT'ATT Cuts at: 5 52 5059 5 Size: 47 5007 2081 Fragments arranged by size: 5007 2081 47 StuI AGG'CCT Cuts at: 1517 3583 1517 Size: 2066 5069 StyI C'CwwG_G Cuts at: 513 1065 2840 3037 3084 3241 3302 4395 4574 Size: 552 1775 197 47 157 61 1093 179 Cuts at: 4574 513 Size: 3074 Fragments arranged by size: 3074 1775 1093 552 197 179 157 61 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3678 4392 4433 5162 5179 5332 5517 Size: 1255 2098 714 41 729 17 153 185 Cuts at: 5517 5962 325 Size: 445 1498 Fragments arranged by size: 2098 1498 1255 729 714 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4345 5381 7093 Size: 274 80 33 51 1443 2368 1036 1712 Cuts at: 7093 96 Size: 138 Fragments arranged by size: 2368 1712 1443 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3427 3459 7095 7127 3427 Size: 32 3636 32 3435 Fragments arranged by size: 3636 3435 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7135 XbaI T'CTAG_A Cuts at: 4471 4471 Size: 7135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7135 XmaI C'CCGG_G Cuts at: 1870 4460 1870 Size: 2590 4545 XmnI GAAnn'nnTTC Cuts at: 5264 5264 Size: 7135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2954 4105 4943 276 Size: 53 83 186 936 1420 1151 838 2468 Fragments arranged by size: 2468 1420 1151 936 838 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI