(Circular) (Six-base) MAPSORT of: pFN21AA0255.seq Check: 3284 from: 1 to: 6580 pFN21AA0255 6580 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2781 2955 4390 278 Size: 53 83 186 936 1245 174 1435 2468 Fragments arranged by size: 2468 1435 1245 936 186 174 83 53 AccI GT'mk_AC Cuts at: 1937 3345 3923 1937 Size: 1408 578 4594 Fragments arranged by size: 4594 1408 578 Acc65I G'GTAC_C Cuts at: 2648 3815 3901 2648 Size: 1167 86 5327 Fragments arranged by size: 5327 1167 86 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2941 3502 4643 6027 6295 1632 Size: 1309 561 1141 1384 268 1917 Fragments arranged by size: 1917 1384 1309 1141 561 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2553 3840 5479 6456 1203 Size: 1350 1287 1639 977 1327 Fragments arranged by size: 1639 1350 1327 1287 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 3299 1166 Size: 2133 4447 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3524 3556 3524 Size: 32 6548 AlwNI CAG_nnn'CTG Cuts at: 4323 5895 4323 Size: 1572 5008 ApaLI G'TGCA_C Cuts at: 2632 2942 4637 5793 2632 Size: 310 1695 1156 3419 Fragments arranged by size: 3419 1695 1156 310 ApoI r'AATT_y Cuts at: 1493 3664 3889 4115 4267 6376 1493 Size: 2171 225 226 152 2109 1697 Fragments arranged by size: 2171 2109 1697 226 225 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 3425 3905 1870 Size: 73 1482 480 4545 Fragments arranged by size: 4545 1482 480 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 3283 3488 3815 3901 Size: 325 217 627 861 635 205 327 86 Cuts at: 3901 5356 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 861 635 627 327 325 217 205 86 BanII G_rGCy'C Cuts at: 1391 2391 3002 3085 3507 3686 3899 1391 Size: 1000 611 83 422 179 213 4072 Fragments arranged by size: 4072 1000 611 422 213 179 83 BbeI G_GCGC'C Cuts at: 3492 3492 Size: 6580 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 2815 2916 2947 928 Size: 971 441 475 101 31 4561 Fragments arranged by size: 4561 971 475 441 101 31 BbvCI CC'TCA_GC Cuts at: 2984 2984 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3220 3254 3304 3338 4771 4805 1903 Size: 34 1283 34 50 34 1433 34 3678 Fragments arranged by size: 3678 1433 1283 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2849 3601 4472 5682 2849 Size: 752 871 1210 3747 Fragments arranged by size: 3747 1210 871 752 BclI T'GATC_A Cuts at: 1548 1548 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2481 3855 6575 2481 Size: 1374 2720 2486 Fragments arranged by size: 2720 2486 1374 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2636 2946 3288 4641 5797 1790 Size: 846 310 342 1353 1156 2573 Fragments arranged by size: 2573 1353 1156 846 342 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2728 2962 4311 Size: 416 53 83 399 24 1311 234 1349 Cuts at: 4311 5269 442 Size: 958 1753 Fragments arranged by size: 1753 1349 1311 958 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2621 3055 5240 1239 Size: 1166 216 434 2185 2579 Fragments arranged by size: 2579 2185 1166 434 216 Bpu10I CC'TnA_GC Cuts at: 2843 2984 3001 2843 Size: 141 17 6422 Fragments arranged by size: 6422 141 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4705 5570 5868 4705 Size: 865 298 5417 Fragments arranged by size: 5417 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2556 4371 493 Size: 711 1352 1815 2702 Fragments arranged by size: 2702 1815 1352 711 BsaBI GATnn'nnATC Cuts at: 3029 6574 3029 Size: 3545 3035 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2778 2952 3489 4387 Size: 53 83 186 936 1245 174 537 898 Cuts at: 4387 4769 5404 275 Size: 382 635 1451 Fragments arranged by size: 1451 1245 936 898 635 537 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 2202 2868 5013 5411 5685 5832 6243 2202 Size: 666 2145 398 274 147 411 2539 Fragments arranged by size: 2539 2145 666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 2915 2945 3524 Size: 30 165 30 1137 30 345 30 579 Cuts at: 3524 3554 1178 Size: 30 4204 Fragments arranged by size: 4204 1137 579 345 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2647 2936 3752 1186 Size: 1461 289 816 4014 Fragments arranged by size: 4014 1461 816 289 BseYI C'CCAG_C Cuts at: 2109 2150 2751 2995 3470 5783 6279 2109 Size: 41 601 244 475 2313 496 2410 Fragments arranged by size: 2410 2313 601 496 475 244 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2284 2456 2474 3657 1163 Size: 573 548 172 18 1183 4086 Fragments arranged by size: 4086 1183 573 548 172 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 2553 2636 2946 3085 3507 4641 4726 5797 Size: 162 83 310 139 422 1134 85 1071 Cuts at: 5797 2391 Size: 3174 Fragments arranged by size: 3174 1134 1071 422 310 162 139 85 83 BsmI GAATG_Cn' Cuts at: 3006 3689 4091 4184 3006 Size: 683 402 93 5402 Fragments arranged by size: 5402 683 402 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2553 2636 2946 3002 3085 3288 Size: 399 601 162 83 310 56 83 203 Cuts at: 3288 3507 3686 3899 4641 4726 5797 1391 Size: 219 179 213 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 399 310 219 213 203 179 162 85 83 83 56 BspEI T'CCGG_A Cuts at: 2868 6243 2868 Size: 3375 3205 BspHI T'CATG_A Cuts at: 2232 3087 4469 6199 2232 Size: 855 1382 1730 2613 Fragments arranged by size: 2613 1730 1382 855 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 2496 3935 844 Size: 1529 123 1439 3489 Fragments arranged by size: 3489 1529 1439 123 BsrBI CCG'CTC Cuts at: 1394 1823 2323 3281 4467 1394 Size: 429 500 958 1186 3507 Fragments arranged by size: 3507 1186 958 500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2352 2877 5075 5249 65 Size: 1183 1104 525 2198 174 1396 Fragments arranged by size: 2198 1396 1183 1104 525 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2454 5224 5411 1700 Size: 110 644 2770 187 2869 Fragments arranged by size: 2869 2770 644 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 2312 4640 5652 2312 Size: 2328 1012 3240 Fragments arranged by size: 3240 2328 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6580 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstXI CCAn_nnnn'nTGG Cuts at: 2383 3301 2383 Size: 918 5662 BstYI r'GATC_y Cuts at: 1972 2481 2784 3327 3420 3474 3855 3910 4277 Size: 509 303 543 93 54 381 55 367 Cuts at: 4277 4662 4679 6131 6217 6229 6575 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 543 509 385 381 367 346 303 93 86 55 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 2643 Size: 6580 BtgI C'CryG_G Cuts at: 513 1065 2916 3297 513 Size: 552 1851 381 3796 Fragments arranged by size: 3796 1851 552 381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2424 487 Size: 683 56 1198 4643 Fragments arranged by size: 4643 1198 683 56 BtsI GCAGTG_nn' Cuts at: 2132 4096 4890 4910 2132 Size: 1964 794 20 3802 Fragments arranged by size: 3802 1964 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3165 3884 3165 Size: 719 5861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2207 2342 5587 809 Size: 507 891 135 3245 1802 Fragments arranged by size: 3245 1802 891 507 135 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2373 3145 Size: 54 1425 50 275 18 486 57 772 Cuts at: 3145 4027 4916 6545 8 Size: 882 889 1629 43 Fragments arranged by size: 1629 1425 889 882 772 486 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 2618 3113 3512 4510 1353 Size: 1179 86 495 399 998 3423 Fragments arranged by size: 3423 1179 998 495 399 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3147 5167 5541 5687 387 Size: 1064 138 1558 2020 374 146 1280 Fragments arranged by size: 2020 1558 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2389 3083 3505 2389 Size: 694 422 5464 Fragments arranged by size: 5464 694 422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2621 2941 3055 3502 4643 5240 Size: 393 773 216 320 114 447 1141 597 Cuts at: 5240 6027 6295 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1141 787 773 597 447 393 320 268 216 114 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 2605 2961 2605 Size: 356 6224 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 EcoRV GAT'ATC Cuts at: 2734 2734 Size: 6580 FspI TGC'GCA Cuts at: 3398 5086 3398 Size: 1688 4892 FspAI rTGC'GCAy Cuts at: 3398 3398 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2022 3492 5727 1678 Size: 344 1470 2235 2531 Fragments arranged by size: 2531 2235 1470 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5221 5253 5295 5327 1373 Size: 32 135 32 3649 32 42 32 2626 Fragments arranged by size: 3649 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2476 3727 3924 4170 669 Size: 988 281 274 264 1251 197 246 3079 Fragments arranged by size: 3079 1251 988 281 274 264 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 3727 4170 3727 Size: 443 6137 KasI G'GCGC_C Cuts at: 3488 3488 Size: 6580 KpnI G_GTAC'C Cuts at: 2652 3819 3905 2652 Size: 1167 86 5327 Fragments arranged by size: 5327 1167 86 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 2421 5694 5878 1305 Size: 993 123 3273 184 2007 Fragments arranged by size: 3273 2007 993 184 123 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 4215 Fragments arranged by size: 4215 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3116 3122 3299 3464 3584 3614 4538 Size: 648 1950 6 177 165 120 30 924 Cuts at: 4538 4897 5056 518 Size: 359 159 2042 Fragments arranged by size: 2042 1950 924 648 359 177 165 159 120 30 6 MspA1I CmG'CkG Cuts at: 1606 2695 3013 3433 3631 3651 3996 4673 5821 Size: 1089 318 420 198 20 345 677 1148 Cuts at: 5821 6066 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1089 677 420 345 318 245 198 20 NarI GG'CG_CC Cuts at: 3489 3489 Size: 6580 NcoI C'CATG_G Cuts at: 513 1065 2916 3297 513 Size: 552 1851 381 3796 Fragments arranged by size: 3796 1851 552 381 NdeI CA'TA_TG Cuts at: 387 3382 387 Size: 2995 3585 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3319 3844 3938 5483 1132 Size: 97 2090 525 94 1545 2229 Fragments arranged by size: 2229 2090 1545 525 97 94 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 3840 5479 3840 Size: 1639 4941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 4649 4681 6162 6194 3524 Size: 32 1093 32 1481 32 3910 Fragments arranged by size: 3910 1481 1093 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2605 Size: 6580 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PstI C_TGCA'G Cuts at: 830 3932 830 Size: 3102 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2695 3013 3631 2695 Size: 318 618 5644 Fragments arranged by size: 5644 618 318 SacI G_AGCT'C Cuts at: 2391 3085 3507 2391 Size: 694 422 5464 Fragments arranged by size: 5464 694 422 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SapI GCTCTTCn'nnn_ Cuts at: 3512 3512 Size: 6580 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SfcI C'TryA_G Cuts at: 826 1045 3928 5063 5744 5935 826 Size: 219 2883 1135 681 191 1471 Fragments arranged by size: 2883 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3490 3490 Size: 6580 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4684 5585 5847 820 Size: 197 926 2741 901 262 1553 Fragments arranged by size: 2741 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6580 StyI C'CwwG_G Cuts at: 513 1065 2682 2916 3297 4019 513 Size: 552 1617 234 381 722 3074 Fragments arranged by size: 3074 1617 722 552 381 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2919 4607 4624 4777 4962 5407 325 Size: 1255 1339 1688 17 153 185 445 1498 Fragments arranged by size: 1688 1498 1339 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 3621 4826 Size: 274 80 33 51 1443 720 924 1205 Cuts at: 4826 6538 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1205 924 720 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2915 2947 6540 6572 2915 Size: 32 3593 32 2923 Fragments arranged by size: 3593 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 3251 3539 4709 3251 Size: 288 1170 5122 Fragments arranged by size: 5122 1170 288 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2779 2953 4388 276 Size: 53 83 186 936 1245 174 1435 2468 Fragments arranged by size: 2468 1435 1245 936 186 174 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI