Restriction map for
pFN21AE0517
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MaeII MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 177 GTAC GT^AC
Aor51HI 374 AGCGCT AGC^GCT
AvaI 99 CCCGAG C^[CT]CG[GA]G
BalI 119 TGGCCA TGG^CCA
BanI 547 GGTGCC G^G[CT][GA]CC
BcnI 165 CCCGG CC^[GC]GG
BlpI 909 GCTGAGC GC^T[ACGT]AGC
BpmI 499 CTGGAG CTGGAG(16/14)
Bpu1102I 909 GCTGAGC GC^T[ACGT]AGC
BseRI 205 GAGGAG GAGGAG(10/8)
BsmBI 49 GAGACG CGTCTC(1/5)
BsoBI 99 CCCGAG C^[CT]CG[GA]G
BstAPI 795 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 909 GCTGAGC GC^T[ACGT]AGC
EarI 451 GAAGAG CTCTTC(1/4)
Eco47III 374 AGCGCT AGC^GCT
EcoNI 750 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 239 GATATC GAT^ATC
EcoT14I 542 CCTTGG C^C[AT][AT]GG
Ksp632I 451 GAAGAG CTCTTC(1/4)
KspI 60 CCGCGG CCGC^GG
MboII 451 GAAGA GAAGA(8/7)
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
NciI 165 CCCGG CC^[GC]GG
NspI 885 GCATGT [GA]CATG^[CT]
PflMI 129 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 161 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 177 GTAC GT^AC
SacII 60 CCGCGG CCGC^GG
SexAI 695 ACCAGGT A^CC[AT]GGT
StyI 542 CCTTGG C^C[AT][AT]GG
TaqI 611 TCGA T^CGA
TfiI 230 GAATC G^A[AT]TC
TthHB8I 611 TCGA T^CGA
Van91I 129 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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ApaI 394 GGGCCC GGGCC^C
537 GGGCCC
AvaII 138 GGACC G^G[AT]CC
162 GGACC
BanII 394 GGGCCC G[GA]GC[CT]^C
537 GGGCCC
BbvCI 458 CCTCAGC CCTCAGC(-5/-2)
632 CCTCAGC
BsgI 30 CTGCAC GTGCAG(16/14)
889 GTGCAG
BsrBI 592 GAGCGG CCGCTC(-3/-3)
832 GAGCGG
BsrI 534 ACTGG ACTGG(1/-1)
622 CCAGT
BstF5I 170 CATCC GGATG(2/0)
675 CATCC
DsaI 60 CCGCGG C^C[GA][CT]GG
616 CCACGG
FokI 170 CATCC GGATG(9/13)
675 CATCC
HgaI 51 GACGC GACGC(5/10)
6 GCGTC
HphI 434 TCACC GGTGA(8/7)
586 TCACC
PleI 873 GACTC GAGTC(4/5)
280 GAGTC
StuI 455 AGGCCT AGG^CCT
776 AGGCCT
Tsp45I 8 GTCAC ^GT[GC]AC
433 GTCAC
TspRI 10 CACTG CA[GC]TG[ACGT][ACGT]^
234 CAGTG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NgoMIV
NlaIII NlaIV PstI PvuII Sau96I
ScrFI SfaNI SfcI TseI