(Circular) (Six-base) MAPSORT of: pFN21AE0517.seq Check: 7324 from: 1 to: 5632 pFN21AE0517 5632 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2437 2450 2479 2437 Size: 13 29 5590 Fragments arranged by size: 5590 29 13 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 2903 3695 5079 5347 1632 Size: 738 533 792 1384 268 1917 Fragments arranged by size: 1917 1384 792 738 533 268 AfeI AGC'GCT Cuts at: 2381 2381 Size: 5632 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2919 1184 Size: 1735 3897 AlwNI CAG_nnn'CTG Cuts at: 2140 2161 2471 2806 2881 3375 4947 2140 Size: 21 310 335 75 494 1572 2825 Fragments arranged by size: 2825 1572 494 335 310 75 21 ApaI G_GGCC'C Cuts at: 2403 2546 2403 Size: 143 5489 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2104 2957 1870 Size: 73 161 853 4545 Fragments arranged by size: 4545 853 161 73 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2552 2953 4408 618 Size: 325 217 627 765 401 1455 1842 Fragments arranged by size: 1842 1455 765 627 401 325 217 BanII G_rGCy'C Cuts at: 1391 2403 2546 2951 1391 Size: 1012 143 405 4072 Fragments arranged by size: 4072 1012 405 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BbvCI CC'TCA_GC Cuts at: 2464 2638 2464 Size: 174 5458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3823 3857 1903 Size: 34 50 34 1802 34 3678 Fragments arranged by size: 3678 1802 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 2915 3049 2915 Size: 134 5498 Bme1580I G_kGCm'C Cuts at: 1790 2403 2546 2557 2845 2860 3693 4849 1790 Size: 613 143 11 288 15 833 1156 2573 Fragments arranged by size: 2573 1156 833 613 288 143 15 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2549 3363 4321 Size: 416 53 83 399 24 1132 814 958 Cuts at: 4321 442 Size: 1753 Fragments arranged by size: 1753 1132 958 814 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2308 2308 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2525 4292 1239 Size: 1286 1767 2579 Fragments arranged by size: 2579 1767 1286 Bpu10I CC'TnA_GC Cuts at: 2464 2516 2638 2464 Size: 52 122 5458 Fragments arranged by size: 5458 122 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 1178 Size: 30 165 30 777 30 4600 Fragments arranged by size: 4600 777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 1186 Size: 1039 4593 BseYI C'CCAG_C Cuts at: 2147 2491 2495 2583 2766 4835 5331 2147 Size: 344 4 88 183 2069 496 2448 Fragments arranged by size: 2448 2069 496 344 183 88 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2020 2915 1163 Size: 573 284 895 3880 Fragments arranged by size: 3880 895 573 284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3693 3778 4849 3693 Size: 85 1071 4476 Fragments arranged by size: 4476 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 BsmBI CGTCTCn'nnnn_ Cuts at: 2048 2048 Size: 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2546 2557 2845 2860 2951 3693 Size: 399 613 143 11 288 15 91 742 Cuts at: 3693 3778 4849 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 399 288 143 91 85 15 11 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2437 2450 2479 2987 844 Size: 1593 13 29 508 3489 Fragments arranged by size: 3489 1593 508 29 13 BsrBI CCG'CTC Cuts at: 1394 1823 2599 2839 3519 1394 Size: 429 776 240 680 3507 Fragments arranged by size: 3507 776 680 429 240 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2358 2482 4276 4463 1700 Size: 110 217 331 124 1794 187 2869 Fragments arranged by size: 2869 1794 331 217 187 124 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 2806 794 Size: 2012 3620 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627 Size: 990 367 385 17 1452 86 12 346 Cuts at: 5627 1972 Size: 1977 Fragments arranged by size: 1977 1452 990 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2065 2621 513 Size: 552 1000 556 3524 Fragments arranged by size: 3524 1000 556 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2014 3148 3942 3962 2014 Size: 1134 794 20 3684 Fragments arranged by size: 3684 1134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2263 2298 529 Size: 35 1699 35 3863 Fragments arranged by size: 3863 1699 35 35 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2152 2624 Size: 54 1425 50 275 18 294 28 472 Cuts at: 2624 2928 3079 3968 5597 8 Size: 304 151 889 1629 43 Fragments arranged by size: 1629 1425 889 472 304 294 275 151 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2451 3562 1353 Size: 1098 1111 3423 Fragments arranged by size: 3423 1111 1098 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2011 4219 4593 4739 387 Size: 1064 138 422 2208 374 146 1280 Fragments arranged by size: 2208 1280 1064 422 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2370 2525 2903 3695 4292 5079 5347 Size: 393 738 155 378 792 597 787 268 Cuts at: 5347 1239 Size: 1524 Fragments arranged by size: 1524 792 787 738 597 393 378 268 155 EcoNI CCTnn'n_nnAGG Cuts at: 2759 2759 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2158 2167 2399 2543 2158 Size: 9 232 144 5247 Fragments arranged by size: 5247 232 144 9 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 EcoRV GAT'ATC Cuts at: 2246 2246 Size: 5632 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2383 2583 2715 2773 4779 1678 Size: 705 200 132 58 2006 2531 Fragments arranged by size: 2531 2006 705 200 132 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 2664 2696 4273 Size: 32 135 32 587 32 473 32 1577 Cuts at: 4273 4305 4347 4379 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1577 587 473 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 3516 Fragments arranged by size: 3516 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 3590 3949 4108 518 Size: 648 2424 359 159 2042 Fragments arranged by size: 2424 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 2137 2151 2495 2776 2803 2914 3048 Size: 461 70 14 344 281 27 111 134 Cuts at: 3048 3725 4873 5118 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 461 344 281 245 134 111 70 27 14 NaeI GCC'GGC Cuts at: 2029 2360 2484 2029 Size: 331 124 5177 Fragments arranged by size: 5177 331 124 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5080 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NgoMIV G'CCGG_C Cuts at: 2027 2358 2482 2027 Size: 331 124 5177 Fragments arranged by size: 5177 331 124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2894 2990 4535 1132 Size: 97 1665 96 1545 2229 Fragments arranged by size: 2229 1665 1545 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2140 1108 Size: 1032 4600 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 2167 Size: 5632 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PspOMI G'GGCC_C Cuts at: 2399 2542 2399 Size: 143 5489 PstI C_TGCA'G Cuts at: 830 2579 2781 2832 2984 830 Size: 1749 202 51 152 3478 Fragments arranged by size: 3478 1749 202 152 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2495 2776 2914 2495 Size: 281 138 5213 Fragments arranged by size: 5213 281 138 SacII CC_GC'GG Cuts at: 2068 2068 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SanDI GG'GwC_CC Cuts at: 2167 2167 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SexAI A'CCwGG_T Cuts at: 2700 2700 Size: 5632 SfcI C'TryA_G Cuts at: 826 1045 2575 2777 2828 2980 4115 4796 4987 Size: 219 1530 202 51 152 1135 681 191 Cuts at: 4987 826 Size: 1471 Fragments arranged by size: 1530 1471 1135 681 219 202 191 152 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 2462 2783 1517 Size: 945 321 4366 Fragments arranged by size: 4366 945 321 StyI C'CwwG_G Cuts at: 513 1065 2547 3071 513 Size: 552 1482 524 3074 Fragments arranged by size: 3074 1482 552 524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 3659 3676 3829 4014 4459 325 Size: 1255 960 1119 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1119 960 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3878 5590 Size: 274 80 33 51 1443 204 1697 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1712 1697 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2431 2463 5592 5624 2431 Size: 32 3129 32 2439 Fragments arranged by size: 3129 2439 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI