Restriction map for
pFN21AE0517
(Linear) (Six-base) MAPSORT of: KIEE0517.seq Check: 9554 from: 1 to: 927
KIEE0517.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 432 445 474 927
Size: 432 13 29 453
Fragments arranged by size:
453 432 29 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 898 927
Size: 365 533 29
Fragments arranged by size:
533 365 29
AfeI AGC'GCT
Cuts at: 0 376 927
Size: 376 551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 914 927
Size: 914 13
AlwNI CAG_nnn'CTG
Cuts at: 0 135 156 466 801 876 927
Size: 135 21 310 335 75 51
Fragments arranged by size:
335 310 135 75 51 21
ApaI G_GGCC'C
Cuts at: 0 398 541 927
Size: 398 143 386
Fragments arranged by size:
398 386 143
AvaI C'yCGr_G
Cuts at: 0 99 927
Size: 99 828
BanI G'GyrC_C
Cuts at: 0 547 927
Size: 547 380
BanII G_rGCy'C
Cuts at: 0 398 541 927
Size: 398 143 386
Fragments arranged by size:
398 386 143
BbvCI CC'TCA_GC
Cuts at: 0 459 633 927
Size: 459 174 294
Fragments arranged by size:
459 294 174
BlpI GC'TnA_GC
Cuts at: 0 910 927
Size: 910 17
Bme1580I G_kGCm'C
Cuts at: 0 398 541 552 840 855 927
Size: 398 143 11 288 15 72
Fragments arranged by size:
398 288 143 72 15 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 927
Size: 544 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 303 927
Size: 303 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 927
Size: 520 407
Bpu10I CC'TnA_GC
Cuts at: 0 459 511 633 927
Size: 459 52 122 294
Fragments arranged by size:
459 294 122 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 927
Size: 175 30 722
Fragments arranged by size:
722 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 927
Size: 220 707
BseYI C'CCAG_C
Cuts at: 0 142 486 490 578 761 927
Size: 142 344 4 88 183 166
Fragments arranged by size:
344 183 166 142 88 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 910 927
Size: 15 895 17
Fragments arranged by size:
895 17 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 43 927
Size: 43 884
Bsp1286I G_dGCh'C
Cuts at: 0 398 541 552 840 855 927
Size: 398 143 11 288 15 72
Fragments arranged by size:
398 288 143 72 15 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 445 474 927
Size: 432 13 29 453
Fragments arranged by size:
453 432 29 13
BsrBI CCG'CTC
Cuts at: 0 594 834 927
Size: 594 240 93
Fragments arranged by size:
594 240 93
BsrFI r'CCGG_y
Cuts at: 0 22 353 477 927
Size: 22 331 124 450
Fragments arranged by size:
450 331 124 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 801 927
Size: 801 126
BtgI C'CryG_G
Cuts at: 0 60 616 927
Size: 60 556 311
Fragments arranged by size:
556 311 60
BtsI GCAGTG_nn'
Cuts at: 0 9 927
Size: 9 918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 258 293 927
Size: 258 35 634
Fragments arranged by size:
634 258 35
EaeI y'GGCC_r
Cuts at: 0 119 147 619 927
Size: 119 28 472 308
Fragments arranged by size:
472 308 119 28
EarI CTCTTCn'nnn_
Cuts at: 0 446 927
Size: 446 481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6 927
Size: 6 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 520 898 927
Size: 365 155 378 29
Fragments arranged by size:
378 365 155 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 927
Size: 754 173
EcoO109I rG'GnC_Cy
Cuts at: 0 153 162 394 538 927
Size: 153 9 232 144 389
Fragments arranged by size:
389 232 153 144 9
EcoRV GAT'ATC
Cuts at: 0 241 927
Size: 241 686
HaeII r_GCGC'y
Cuts at: 0 378 578 710 768 927
Size: 378 200 132 58 159
Fragments arranged by size:
378 200 159 132 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 659 691 927
Size: 154 32 473 32 236
Fragments arranged by size:
473 236 154 32 32
MscI TGG'CCA
Cuts at: 0 121 927
Size: 121 806
MspA1I CmG'CkG
Cuts at: 0 62 132 146 490 771 798 909 927
Size: 62 70 14 344 281 27 111 18
Fragments arranged by size:
344 281 111 70 62 27 18 14
NaeI GCC'GGC
Cuts at: 0 24 355 479 927
Size: 24 331 124 448
Fragments arranged by size:
448 331 124 24
NgoMIV G'CCGG_C
Cuts at: 0 22 353 477 927
Size: 22 331 124 450
Fragments arranged by size:
450 331 124 22
NspI r_CATG'y
Cuts at: 0 889 927
Size: 889 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 927
Size: 135 792
PpuMI rG'GwC_Cy
Cuts at: 0 162 927
Size: 162 765
PspOMI G'GGCC_C
Cuts at: 0 394 537 927
Size: 394 143 390
Fragments arranged by size:
394 390 143
PstI C_TGCA'G
Cuts at: 0 574 776 827 927
Size: 574 202 51 100
Fragments arranged by size:
574 202 100 51
PvuII CAG'CTG
Cuts at: 0 490 771 909 927
Size: 490 281 138 18
Fragments arranged by size:
490 281 138 18
SacII CC_GC'GG
Cuts at: 0 63 927
Size: 63 864
SanDI GG'GwC_CC
Cuts at: 0 162 927
Size: 162 765
SexAI A'CCwGG_T
Cuts at: 0 695 927
Size: 695 232
SfcI C'TryA_G
Cuts at: 0 570 772 823 927
Size: 570 202 51 104
Fragments arranged by size:
570 202 104 51
StuI AGG'CCT
Cuts at: 0 457 778 927
Size: 457 321 149
Fragments arranged by size:
457 321 149
StyI C'CwwG_G
Cuts at: 0 542 927
Size: 542 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 927
Size: 535 392
TatI w'GTAC_w
Cuts at: 0 176 927
Size: 176 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 927
Size: 426 32 469
Fragments arranged by size:
469 426 32
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI ApaI AvaI BanI
BanII BbvCI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI
BsrFI BstAPI BtgI BtsI CspCI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I MscI MspA1I
NaeI NgoMIV NspI PflMI PpuMI PspOMI PstI PvuII
SacII SanDI SexAI SfcI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI