(Circular) (Six-base) MAPSORT of: pFN21AB7671.seq Check: 5282 from: 1 to: 5719 pFN21AB7671 5719 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2518 2575 2667 2518 Size: 57 92 5570 Fragments arranged by size: 5570 92 57 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 5166 5434 1632 Size: 2150 1384 268 1917 Fragments arranged by size: 2150 1917 1384 268 AfeI AGC'GCT Cuts at: 2348 2501 2348 Size: 153 5566 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2550 4618 5595 1203 Size: 1347 2068 977 1327 Fragments arranged by size: 2068 1347 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 2819 1166 Size: 1653 4066 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 2362 2488 2659 2674 2866 3462 5034 2362 Size: 126 171 15 192 596 1572 3047 Fragments arranged by size: 3047 1572 596 192 171 126 15 ApaI G_GGCC'C Cuts at: 2768 2768 Size: 5719 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2117 3044 1870 Size: 73 174 927 4545 Fragments arranged by size: 4545 927 174 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2035 2846 3040 4495 Size: 325 217 627 233 15 811 194 1455 Cuts at: 4495 618 Size: 1842 Fragments arranged by size: 1842 1455 811 627 325 233 217 194 15 BanII G_rGCy'C Cuts at: 1391 2088 2768 3038 1391 Size: 697 680 270 4072 Fragments arranged by size: 4072 697 680 270 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2318 928 Size: 971 419 4329 Fragments arranged by size: 4329 971 419 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 2252 2363 3136 2252 Size: 111 773 4835 Fragments arranged by size: 4835 773 111 Bme1580I G_kGCm'C Cuts at: 1790 2025 2040 2768 2849 3780 4936 1790 Size: 235 15 728 81 931 1156 2573 Fragments arranged by size: 2573 1156 931 728 235 81 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3450 4408 442 Size: 416 53 83 399 24 2033 958 1753 Fragments arranged by size: 2033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2267 2267 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2321 2540 2561 2660 2888 4379 1239 Size: 1016 66 219 21 99 228 1491 2579 Fragments arranged by size: 2579 1491 1016 228 219 99 66 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 2327 2552 3844 4709 5007 2201 Size: 126 225 1292 865 298 2913 Fragments arranged by size: 2913 1292 865 298 225 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2970 3526 3908 4543 Size: 53 83 186 936 1437 556 382 635 Cuts at: 4543 275 Size: 1451 Fragments arranged by size: 1451 1437 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2279 2309 2927 2957 2981 Size: 30 165 30 876 30 618 30 24 Cuts at: 2981 3011 1178 Size: 30 3886 Fragments arranged by size: 3886 876 618 165 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 2718 2721 2761 2885 2888 3005 1186 Size: 1351 181 3 40 124 3 117 3900 Fragments arranged by size: 3900 1351 181 124 117 40 3 3 BseYI C'CCAG_C Cuts at: 2667 4922 5418 2667 Size: 2255 496 2968 Fragments arranged by size: 2968 2255 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 2843 1163 Size: 573 873 234 4039 Fragments arranged by size: 4039 873 573 234 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3780 3865 4936 3780 Size: 85 1071 4563 Fragments arranged by size: 4563 1071 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 BsmBI CGTCTCn'nnnn_ Cuts at: 2173 2173 Size: 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2040 2088 2768 2849 3038 3780 Size: 399 235 15 48 680 81 189 742 Cuts at: 3780 3865 4936 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 680 399 235 189 85 81 48 15 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2463 2518 2575 2667 3074 844 Size: 1619 55 57 92 407 3489 Fragments arranged by size: 3489 1619 407 92 57 55 BsrBI CCG'CTC Cuts at: 1394 1823 2140 2176 2367 2644 3606 1394 Size: 429 317 36 191 277 962 3507 Fragments arranged by size: 3507 962 429 317 277 191 36 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2587 2659 794 Size: 1793 72 3854 Fragments arranged by size: 3854 1793 72 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstYI r'GATC_y Cuts at: 1972 3049 3416 3801 3818 5270 5356 5368 5714 Size: 1077 367 385 17 1452 86 12 346 Cuts at: 5714 1972 Size: 1977 Fragments arranged by size: 1977 1452 1077 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2703 2760 2973 513 Size: 552 939 699 57 213 3259 Fragments arranged by size: 3259 939 699 552 213 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 3235 4029 4049 3235 Size: 794 20 4905 Fragments arranged by size: 4905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684 Size: 54 1425 50 275 18 1336 889 1629 Cuts at: 5684 8 Size: 43 Fragments arranged by size: 1629 1425 1336 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3649 1353 Size: 2296 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2158 2462 4306 4680 4826 387 Size: 1064 138 569 304 1844 374 146 1280 Fragments arranged by size: 1844 1280 1064 569 374 304 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2321 2540 2561 2660 2888 3782 Size: 393 623 66 219 21 99 228 894 Cuts at: 3782 4379 5166 5434 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 894 787 623 597 393 268 228 219 99 66 21 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2303 Size: 5719 EcoO109I rG'GnC_Cy Cuts at: 2452 2765 2923 2452 Size: 313 158 5248 Fragments arranged by size: 5248 313 158 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FspI TGC'GCA Cuts at: 2510 2580 4225 2510 Size: 70 1645 4004 Fragments arranged by size: 4004 1645 70 FspAI rTGC'GCAy Cuts at: 2510 2510 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2350 2503 4866 1678 Size: 672 153 2363 2531 Fragments arranged by size: 2531 2363 672 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2877 2909 2927 2959 2981 Size: 32 135 32 1305 32 18 32 22 Cuts at: 2981 3013 4360 4392 4434 4466 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 1305 135 42 32 32 32 32 32 32 32 22 18 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2970 4833 5017 1305 Size: 1665 1863 184 2007 Fragments arranged by size: 2007 1863 1665 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3897 Fragments arranged by size: 3897 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2748 2819 3677 4036 4195 518 Size: 648 1582 71 858 359 159 2042 Fragments arranged by size: 2042 1582 858 648 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2128 2488 2593 2881 2913 3135 3812 4960 Size: 522 360 105 288 32 222 677 1148 Cuts at: 4960 5205 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 522 360 288 245 222 105 32 NcoI C'CATG_G Cuts at: 513 1065 2004 2760 2973 513 Size: 552 939 756 213 3259 Fragments arranged by size: 3259 939 756 552 213 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2188 2554 3077 4622 1132 Size: 97 959 366 523 1545 2229 Fragments arranged by size: 2229 1545 959 523 366 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 2550 4618 2550 Size: 2068 3651 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PfoI T'CCnGG_A Cuts at: 2355 2355 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 5719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2764 2764 Size: 5719 PstI C_TGCA'G Cuts at: 830 2598 3071 830 Size: 1768 473 3478 Fragments arranged by size: 3478 1768 473 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2488 2593 2913 2488 Size: 105 320 5294 Fragments arranged by size: 5294 320 105 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 2089 2594 3067 4202 4883 5074 826 Size: 219 1044 505 473 1135 681 191 1471 Fragments arranged by size: 1471 1135 1044 681 505 473 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2342 2567 3823 4724 4986 Size: 197 926 273 126 225 1256 901 262 Cuts at: 4986 820 Size: 1553 Fragments arranged by size: 1553 1256 926 901 273 262 225 197 126 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 2188 3077 1132 Size: 1056 889 3774 Fragments arranged by size: 3774 1056 889 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 2957 1517 Size: 1440 4279 StyI C'CwwG_G Cuts at: 513 1065 2004 2760 2973 3158 513 Size: 552 939 756 213 185 3074 Fragments arranged by size: 3074 939 756 552 213 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 2764 2864 3746 3763 3916 4101 Size: 1255 1128 56 100 882 17 153 185 Cuts at: 4101 4546 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1128 882 445 185 153 100 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 3965 5677 Size: 274 80 33 51 1443 309 1679 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1679 1443 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI