Restriction map for
pFN21AB6667
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I FauI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflII 486 CTTAAG C^TTAAG
Alw44I 614 GTGCAC G^TGCAC
AlwI 429 GGATC GGATC(4/5)
AlwNI 506 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 586 CCTCAGG CC^T[ACGT]AGG
Aor51HI 573 AGCGCT AGC^GCT
ApaLI 614 GTGCAC G^TGCAC
BcgI 1170 GCATATACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 273 TGATCA T^GATCA
BfrI 486 CTTAAG C^TTAAG
BsaAI 122 TACGTG [CT]AC^GT[GA]
BsgI 651 GTGCAG GTGCAG(16/14)
BstYI 429 GGATCT [GA]^GATC[CT]
Bsu36I 586 CCTCAGG CC^T[ACGT]AGG
CfoI 574 GCGC GCG^C
Eco47III 573 AGCGCT AGC^GCT
Eco81I 586 CCTCAGG CC^T[ACGT]AGG
FbaI 273 TGATCA T^GATCA
HaeII 573 AGCGCT [GA]GCGC^[CT]
HhaI 574 GCGC GCG^C
HinP1I 574 GCGC G^CGC
MflI 429 GGATCT [GA]^GATC[CT]
MslI 1258 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 139 CAGCTG C[AC]G^C[GT]G
NheI 462 GCTAGC G^CTAGC
NlaIV 1035 GGACCC GG[ACGT]^[ACGT]CC
PaeR7I 1178 CTCGAG C^TCGAG
PstI 74 CTGCAG CTGCA^G
PvuII 139 CAGCTG CAG^CTG
SfcI 74 CTGCAG C^T[GA][CT]AG
SmaI 1071 CCCGGG CCC^GGG
XhoI 1178 CTCGAG C^TCGAG
XhoII 429 GGATCT [GA]^GATC[CT]
XmaCI 1071 CCCGGG C^CCGGG
XmaI 1071 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 548 GTAC GT^AC
1144 GTAC
Asp700I 417 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
439 GAAGAATTTC
AspHI 528 GTGCTC G[AT]GC[AT]^C
614 GTGCAC
AvaI 1071 CCCGGG C^[CT]CG[GA]G
1178 CTCGAG
AvaII 1035 GGACC G^G[AT]CC
1090 GGACC
BbvI 1098 GCAGC GCAGC(8/12)
976 GCTGC
BfaI 353 CTAG C^TAG
463 CTAG
BmyI 528 GTGCTC G[AGT]GC[ACT]^C
614 GTGCAC
BpmI 746 CTCCAG CTGGAG(16/14)
992 CTGGAG
BsaI 753 GAGACC GGTCTC(1/5)
995 GAGACC
BsiHKAI 528 GTGCTC G[AT]GC[AT]^C
614 GTGCAC
BsiYI 396 CCATGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1026 CCCACTTCTGG
BslI 396 CCATGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1026 CCCACTTCTGG
BsoBI 1071 CCCGGG C^[CT]CG[GA]G
1178 CTCGAG
Bsp1286I 528 GTGCTC G[AGT]GC[ACT]^C
614 GTGCAC
Cfr13I 1035 GGACC G^G[ACGT]CC
1090 GGACC
DpnII 274 GATC ^GATC
430 GATC
EcoRI 195 GAATTC G^AATTC
1189 GAATTC
Fnu4HI 976 GCTGC GC^[ACGT]GC
1098 GCAGC
ItaI 976 GCTGC GC^[ACGT]GC
1098 GCAGC
MaeI 353 CTAG C^TAG
463 CTAG
MaeII 123 ACGT A^CGT
778 ACGT
MboI 274 GATC ^GATC
430 GATC
MwoI 34 GCGTATGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
576 GCTGTTTAAGC
NdeII 274 GATC ^GATC
430 GATC
NspI 55 GCATGC [GA]CATG^[CT]
458 GCATGC
RsaI 548 GTAC GT^AC
1144 GTAC
Sau3AI 274 GATC ^GATC
430 GATC
Sau96I 1035 GGACC G^G[ACGT]CC
1090 GGACC
SphI 55 GCATGC GCATG^C
458 GCATGC
TaqI 1064 TCGA T^CGA
1179 TCGA
TseI 976 GCTGC G^C[AT]GC
1098 GCAGC
TthHB8I 1064 TCGA T^CGA
1179 TCGA
XmnI 417 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
439 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI BbsI BcnI
BpuAI BsaJI BsmAI BssKI BstF5I
BstNI Cac8I DdeI EcoRII EcoT22I
FokI HapII HinfI HpaII MaeIII
MboII MnlI MseI MspI MvaI
NciI NlaIII NsiI PleI ScrFI
SfaNI SmlI TfiI Tru9I Tsp45I
Tsp509I TspRI