Restriction map for
pFN21AB6667
(Linear) (Six-base) MAPSORT of: KIBB6667.seq Check: 4563 from: 1 to: 1275
KIBB6667.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 875 1275
Size: 350 525 400
Fragments arranged by size:
525 400 350
AfeI AGC'GCT
Cuts at: 0 575 1275
Size: 575 700
AflII C'TTAA_G
Cuts at: 0 486 1275
Size: 486 789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 1275
Size: 406 869
AlwNI CAG_nnn'CTG
Cuts at: 0 511 1275
Size: 511 764
ApaLI G'TGCA_C
Cuts at: 0 614 1275
Size: 614 661
ApoI r'AATT_y
Cuts at: 0 195 442 691 802 866 1189 1275
Size: 195 247 249 111 64 323 86
Fragments arranged by size:
323 249 247 195 111 86 64
AvaI C'yCGr_G
Cuts at: 0 1071 1178 1275
Size: 1071 107 97
Fragments arranged by size:
1071 107 97
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 936 969 1275
Size: 936 33 306
Fragments arranged by size:
936 306 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 339 424 671 1275
Size: 245 94 85 247 604
Fragments arranged by size:
604 247 245 94 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1193 1275
Size: 1159 34 82
Fragments arranged by size:
1159 82 34
BclI T'GATC_A
Cuts at: 0 273 1275
Size: 273 1002
BfrBI ATG'CAT
Cuts at: 0 59 458 1006 1275
Size: 59 399 548 269
Fragments arranged by size:
548 399 269 59
Bme1580I G_kGCm'C
Cuts at: 0 618 1275
Size: 618 657
BmgBI CAC'GTC
Cuts at: 0 779 1275
Size: 779 496
BmtI G_CTAG'C
Cuts at: 0 466 1275
Size: 466 809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 1013 1275
Size: 731 282 262
Fragments arranged by size:
731 282 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1275
Size: 188 1087
BsaI GGTCTCn'nnnn_
Cuts at: 0 747 989 1275
Size: 747 242 286
Fragments arranged by size:
747 286 242
BsaAI yAC'GTr
Cuts at: 0 124 1275
Size: 124 1151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 1275
Size: 672 603
BsiHKAI G_wGCw'C
Cuts at: 0 532 618 1275
Size: 532 86 657
Fragments arranged by size:
657 532 86
Bsp1286I G_dGCh'C
Cuts at: 0 532 618 1275
Size: 532 86 657
Fragments arranged by size:
657 532 86
BstYI r'GATC_y
Cuts at: 0 429 1275
Size: 429 846
Bsu36I CC'TnA_GG
Cuts at: 0 587 1275
Size: 587 688
BtsI GCAGTG_nn'
Cuts at: 0 509 794 1275
Size: 509 285 481
Fragments arranged by size:
509 481 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 731 875 1013 1275
Size: 350 381 144 138 262
Fragments arranged by size:
381 350 262 144 138
EcoRI G'AATT_C
Cuts at: 0 195 1189 1275
Size: 195 994 86
Fragments arranged by size:
994 195 86
HaeII r_GCGC'y
Cuts at: 0 577 1275
Size: 577 698
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 258 290 709 741 746 778 1120 1152
Size: 258 32 419 32 5 32 342 32
Cuts at: 1152 1275
Size: 123
Fragments arranged by size:
419 342 258 123 32 32 32 32
5
MslI CAynn'nnrTG
Cuts at: 0 1262 1275
Size: 1262 13
MspA1I CmG'CkG
Cuts at: 0 141 1275
Size: 141 1134
NheI G'CTAG_C
Cuts at: 0 462 1275
Size: 462 813
NsiI A_TGCA'T
Cuts at: 0 61 460 1008 1275
Size: 61 399 548 267
Fragments arranged by size:
548 399 267 61
NspI r_CATG'y
Cuts at: 0 59 462 1275
Size: 59 403 813
Fragments arranged by size:
813 403 59
PasI CC'CwG_GG
Cuts at: 0 829 1275
Size: 829 446
PspXI vC'TCGA_Gb
Cuts at: 0 1178 1275
Size: 1178 97
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1157 1189 1275
Size: 1157 32 86
Fragments arranged by size:
1157 86 32
PstI C_TGCA'G
Cuts at: 0 78 1275
Size: 78 1197
PvuII CAG'CTG
Cuts at: 0 141 1275
Size: 141 1134
SfcI C'TryA_G
Cuts at: 0 74 1275
Size: 74 1201
SmaI CCC'GGG
Cuts at: 0 1073 1275
Size: 1073 202
SmlI C'TyrA_G
Cuts at: 0 203 486 1178 1275
Size: 203 283 692 97
Fragments arranged by size:
692 283 203 97
SphI G_CATG'C
Cuts at: 0 59 462 1275
Size: 59 403 813
Fragments arranged by size:
813 403 59
XhoI C'TCGA_G
Cuts at: 0 1178 1275
Size: 1178 97
XmaI C'CCGG_G
Cuts at: 0 1071 1275
Size: 1071 204
XmnI GAAnn'nnTTC
Cuts at: 0 421 443 1275
Size: 421 22 832
Fragments arranged by size:
832 421 22
Enzymes that do cut:
AcuI AfeI AflII AlfI AlwNI ApaLI ApoI AvaI
BaeI BbsI BcgI BclI BfrBI Bme1580I BmgBI BmtI
BpmI BpuEI BsaI BsaAI BsgI BsiHKAI Bsp1286I BstYI
Bsu36I BtsI Eco57MI EcoRI HaeII Hin4I MslI MspA1I
NheI NsiI NspI PasI PspXI PsrI PstI PvuII
SfcI SmaI SmlI SphI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BglI BglII BlpI
BmrI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI ZraI