Restriction map for
pFN21AB7319
(Circular) (Six-base) MAPSORT of: pFN21AB7319.seq Check: 4208 from: 1 to: 7222
pFN21AB7319 7222 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2158 3239 3899 2158
Size: 1081 660 5481
Fragments arranged by size:
5481 1081 660
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2756 5032 278
Size: 53 83 186 936 1220 2276 2468
Fragments arranged by size:
2468 2276 1220 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3159 3249 4565 1937
Size: 1222 90 1316 4594
Fragments arranged by size:
4594 1316 1222 90
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 5349 5722 1563
Size: 3786 373 3063
Fragments arranged by size:
3786 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2992 3449 3612 5285 6669 6937 1632
Size: 1360 457 163 1673 1384 268 1917
Fragments arranged by size:
1917 1673 1384 1360 457 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7025
AflIII A'CryG_T
Cuts at: 1203 6121 7098 1203
Size: 4918 977 1327
Fragments arranged by size:
4918 1327 977
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 4373 1166
Size: 3207 4015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2285 4202 4274 1184
Size: 1101 1917 72 4132
Fragments arranged by size:
4132 1917 1101 72
AlwNI CAG_nnn'CTG
Cuts at: 2773 4017 4965 6537 2773
Size: 1244 948 1572 3458
Fragments arranged by size:
3458 1572 1244 948
ApaI G_GGCC'C
Cuts at: 2013 3032 3809 4038 2013
Size: 1019 777 229 5197
Fragments arranged by size:
5197 1019 777 229
ApaLI G'TGCA_C
Cuts at: 5279 6435 5279
Size: 1156 6066
ApoI r'AATT_y
Cuts at: 1493 4531 4757 4909 7018 1493
Size: 3038 226 152 2109 1697
Fragments arranged by size:
3038 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 3001 4547 1870
Size: 73 1058 1546 4545
Fragments arranged by size:
4545 1546 1058 73
BamHI G'GATC_C
Cuts at: 4552 4919 4552
Size: 367 6855
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2888 2963 3498 3572
Size: 325 217 627 383 718 75 535 74
Cuts at: 3572 3694 4543 5998 618
Size: 122 849 1455 1842
Fragments arranged by size:
1842 1455 849 718 627 535 383 325
217 122 75 74
BanII G_rGCy'C
Cuts at: 1391 2013 3032 3809 4038 4541 1391
Size: 622 1019 777 229 503 4072
Fragments arranged by size:
4072 1019 777 622 503 229
BbeI G_GCGC'C
Cuts at: 2892 2892
Size: 7222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3667 928
Size: 971 1768 4483
Fragments arranged by size:
4483 1768 971
BbvCI CC'TCA_GC
Cuts at: 3053 3737 4295 3053
Size: 684 558 5980
Fragments arranged by size:
5980 684 558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5114 6324 5114
Size: 1210 6012
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7222
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2961 3213 3570
Size: 107 122 71 631 605 1289 252 357
Cuts at: 3570 3719 5833 136
Size: 149 2114 1525
Fragments arranged by size:
2114 1525 1289 631 605 357 252 149
122 107 71
BglII A'GATC_T
Cuts at: 3273 7217 3273
Size: 3944 3278
BlpI GC'TnA_GC
Cuts at: 2061 4639 2061
Size: 2578 4644
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2175 2966 2997 3032 3809 4038 5283
Size: 223 162 791 31 35 777 229 1245
Cuts at: 5283 6439 1790
Size: 1156 2573
Fragments arranged by size:
2573 1245 1156 791 777 229 223 162
35 31
BmgBI CAC'GTC
Cuts at: 1318 3334 3538 1318
Size: 2016 204 5002
Fragments arranged by size:
5002 2016 204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4027 4953 5911
Size: 416 53 83 399 24 2610 926 958
Cuts at: 5911 442
Size: 1753
Fragments arranged by size:
2610 1753 958 926 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3787 5882 1239
Size: 2548 2095 2579
Fragments arranged by size:
2579 2548 2095
Bpu10I CC'TnA_GC
Cuts at: 3053 3737 4295 3053
Size: 684 558 5980
Fragments arranged by size:
5980 684 558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3287 5347 6212 6510 3287
Size: 2060 865 298 3999
Fragments arranged by size:
3999 2060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3641 3889 5885 882
Size: 617 2142 248 1996 2219
Fragments arranged by size:
2219 2142 1996 617 248
BsaAI yAC'GTr
Cuts at: 493 1204 3868 5013 493
Size: 711 2664 1145 2702
Fragments arranged by size:
2702 2664 1145 711
BsaBI GATnn'nnATC
Cuts at: 7216 7216
Size: 7222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2753 2889 5029 5411
Size: 53 83 186 936 1220 136 2140 382
Cuts at: 5411 6046 275
Size: 635 1451
Fragments arranged by size:
2140 1451 1220 936 635 382 186 136
83 53
BsaWI w'CCGG_w
Cuts at: 3372 5655 6053 6327 6474 6885 3372
Size: 2283 398 274 147 411 3709
Fragments arranged by size:
3709 2283 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 1178
Size: 30 165 30 1047 30 5920
Fragments arranged by size:
5920 1047 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2258 2774 3200 3284 4528 1186
Size: 834 238 516 426 84 1244 3880
Fragments arranged by size:
3880 1244 834 516 426 238 84
BseYI C'CCAG_C
Cuts at: 2284 2558 3038 3800 6425 6921 2284
Size: 274 480 762 2625 496 2585
Fragments arranged by size:
2625 2585 762 496 480 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2585 2609 2918 3185 3217 3326 4318
Size: 573 849 24 309 267 32 109 992
Cuts at: 4318 1163
Size: 4067
Fragments arranged by size:
4067 992 849 573 309 267 109 32
24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4672 5433 6461 1540
Size: 275 186 2671 761 1028 2301
Fragments arranged by size:
2671 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2807 4398 5283 5368 6439 2340
Size: 467 1591 885 85 1071 3123
Fragments arranged by size:
3123 1591 1071 885 467 85
BsmI GAATG_Cn'
Cuts at: 3611 4733 4826 3611
Size: 1122 93 6007
Fragments arranged by size:
6007 1122 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 4048 4330 2711
Size: 1337 282 5603
Fragments arranged by size:
5603 1337 282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2175 2340 2807 2966 2997 3032
Size: 399 223 162 165 467 159 31 35
Cuts at: 3032 3809 4038 4398 4541 5283 5368 6439 1391
Size: 777 229 360 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 777 742 467 399 360 229
223 165 162 159 143 85 35 31
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 2445 5111 6841 2445
Size: 2666 1730 2826
Fragments arranged by size:
2826 2666 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2158 3086 3239 3899 4218 4577 844
Size: 1253 61 928 153 660 319 359 3489
Fragments arranged by size:
3489 1253 928 660 359 319 153 61
BsrBI CCG'CTC
Cuts at: 1394 1823 5109 1394
Size: 429 3286 3507
Fragments arranged by size:
3507 3286 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3371 5717 5891 65
Size: 1183 2123 2346 174 1396
Fragments arranged by size:
2346 2123 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2332 5866 6053 1700
Size: 110 255 267 3534 187 2869
Fragments arranged by size:
3534 2869 267 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7222
BssHII G'CGCG_C
Cuts at: 1925 2810 1925
Size: 885 6337
BssSI C'ACGA_G
Cuts at: 2354 5282 6294 2354
Size: 2928 1012 3282
Fragments arranged by size:
3282 2928 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 2596 3561 794
Size: 1433 369 965 4455
Fragments arranged by size:
4455 1433 965 369
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 2776 4366 1184
Size: 1592 1590 4040
Fragments arranged by size:
4040 1592 1590
BstXI CCAn_nnnn'nTGG
Cuts at: 2672 3244 3709 4417 2672
Size: 572 465 708 5477
Fragments arranged by size:
5477 708 572 465
BstYI r'GATC_y
Cuts at: 1972 3273 4552 4919 5304 5321 6773 6859 6871
Size: 1301 1279 367 385 17 1452 86 12
Cuts at: 6871 7217 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1301 1279 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2583 4101 513
Size: 552 939 579 1518 3634
Fragments arranged by size:
3634 1518 939 579 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2523 2689 3688 487
Size: 683 56 1297 166 999 4021
Fragments arranged by size:
4021 1297 999 683 166 56
BtsI GCAGTG_nn'
Cuts at: 2729 3830 4738 5532 5552 2729
Size: 1101 908 794 20 4399
Fragments arranged by size:
4399 1101 908 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7187
DraI TTT'AAA
Cuts at: 4526 4526
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4385 6229 809
Size: 507 3069 1844 1802
Fragments arranged by size:
3069 1844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 2650 2662
Size: 54 1425 50 275 18 624 196 12
Cuts at: 2662 3720 3747 3987 4098 4407 4669 5558 7187
Size: 1058 27 240 111 309 262 889 1629
Cuts at: 7187 8
Size: 43
Fragments arranged by size:
1629 1425 1058 889 624 309 275 262
240 196 111 54 50 43 27 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 4669 1537
Size: 275 2857 4090
Fragments arranged by size:
4090 2857 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 4457 5152 1353
Size: 770 2334 695 3423
Fragments arranged by size:
3423 2334 770 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5809 6183 6329 387
Size: 1064 138 4220 374 146 1280
Fragments arranged by size:
4220 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2992 3449 3612 3787 5285 5882 6669
Size: 393 1360 457 163 175 1498 597 787
Cuts at: 6669 6937 1239
Size: 268 1524
Fragments arranged by size:
1524 1498 1360 787 597 457 393 268
175 163
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 2219
Size: 7222
EcoO109I rG'GnC_Cy
Cuts at: 2009 2053 3028 3805 3806 3907 4034 4035 2009
Size: 44 975 777 1 101 127 1 5196
Fragments arranged by size:
5196 975 777 127 101 44 1 1
EcoRI G'AATT_C
Cuts at: 4531 4909 4531
Size: 378 6844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 3304 2548
Size: 756 6466
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 2502 2565 2767 2773 2892 6369 1678
Size: 824 63 202 6 119 3477 2531
Fragments arranged by size:
3477 2531 824 202 119 63 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3367 3399 5863 5895 5937
Size: 32 135 32 1795 32 2464 32 42
Cuts at: 5937 5969 1373
Size: 32 2626
Fragments arranged by size:
2626 2464 1795 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3286 4566 4812 669
Size: 988 281 1348 1280 246 3079
Fragments arranged by size:
3079 1348 1280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7222
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KasI G'GCGC_C
Cuts at: 2888 2888
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 4152 4821 4152
Size: 669 6553
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 6336 6520 1305
Size: 1038 3993 184 2007
Fragments arranged by size:
3993 2007 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 2664 3749 3989 4100
Size: 54 1425 343 624 208 1085 240 111
Cuts at: 4100 4409 10
Size: 309 2823
Fragments arranged by size:
2823 1425 1085 624 343 309 240 208
111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 3707 3755 4340 4373 5180 5539
Size: 648 1284 1257 48 585 33 807 359
Cuts at: 5539 5698 518
Size: 159 2042
Fragments arranged by size:
2042 1284 1257 807 648 585 359 159
48 33
MspA1I CmG'CkG
Cuts at: 1606 2593 2610 2668 3262 3427 3670 4356 4638
Size: 987 17 58 594 165 243 686 282
Cuts at: 4638 5315 6463 6708 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 987 686 677 594 282 245
243 165 58 17
NaeI GCC'GGC
Cuts at: 2067 2067
Size: 7222
NarI GG'CG_CC
Cuts at: 2889 2889
Size: 7222
NcoI C'CATG_G
Cuts at: 513 1065 2004 2583 513
Size: 552 939 579 5152
Fragments arranged by size:
5152 939 579 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7222
NgoMIV G'CCGG_C
Cuts at: 2065 2065
Size: 7222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 4096 4580 6125 1132
Size: 97 2867 484 1545 2229
Fragments arranged by size:
2867 2229 1545 484 97
PasI CC'CwG_GG
Cuts at: 1348 2013 3727 1348
Size: 665 1714 4843
Fragments arranged by size:
4843 1714 665
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 3038 1108
Size: 1665 265 5292
Fragments arranged by size:
5292 1665 265
PfoI T'CCnGG_A
Cuts at: 2712 3297 3678 2712
Size: 585 381 6256
Fragments arranged by size:
6256 585 381
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5291 5323 6804 6836 4268
Size: 32 991 32 1481 32 4654
Fragments arranged by size:
4654 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2053
Size: 7222
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 4792 4792
Size: 7222
PspOMI G'GGCC_C
Cuts at: 2009 3028 3805 4034 2009
Size: 1019 777 229 5197
Fragments arranged by size:
5197 1019 777 229
PstI C_TGCA'G
Cuts at: 830 2169 2736 2799 3420 4574 830
Size: 1339 567 63 621 1154 3478
Fragments arranged by size:
3478 1339 1154 621 567 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
PvuII CAG'CTG
Cuts at: 2593 2668 3262 3427 3670 2593
Size: 75 594 165 243 6145
Fragments arranged by size:
6145 594 243 165 75
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SbfI CC_TGCA'GG
Cuts at: 2169 4574 2169
Size: 2405 4817
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SfcI C'TryA_G
Cuts at: 826 1045 2165 2386 2494 2732 2795 3160 3416
Size: 219 1120 221 108 238 63 365 256
Cuts at: 3416 4009 4351 4570 5705 6386 6577 826
Size: 593 342 219 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 593 365 342 256
238 221 219 219 191 108 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 3719 3719
Size: 7222
SfoI GGC'GCC
Cuts at: 2890 2890
Size: 7222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 3003 4549 1872
Size: 1131 1546 4545
Fragments arranged by size:
4545 1546 1131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3266 5326 6227 6489 820
Size: 197 926 1323 2060 901 262 1553
Fragments arranged by size:
2060 1553 1323 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4096 4580 1132
Size: 2964 484 3774
Fragments arranged by size:
3774 2964 484
SspI AAT'ATT
Cuts at: 5 52 5146 5
Size: 47 5094 2081
Fragments arranged by size:
5094 2081 47
StuI AGG'CCT
Cuts at: 1517 3614 1517
Size: 2097 5125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 3879 4661 513
Size: 552 939 579 1296 782 3074
Fragments arranged by size:
3074 1296 939 782 579 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2572 3712 3799 5249 5266 5419
Size: 1255 519 473 1140 87 1450 17 153
Cuts at: 5419 5604 6049 325
Size: 185 445 1498
Fragments arranged by size:
1498 1450 1255 1140 519 473 445 185
153 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3168 5468 7180
Size: 274 80 33 51 1443 1191 2300 1712
Cuts at: 7180 96
Size: 138
Fragments arranged by size:
2300 1712 1443 1191 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7182 7214 7182
Size: 32 7190
Tth111I GACn'n_nGTC
Cuts at: 1316 2927 1316
Size: 1611 5611
XbaI T'CTAG_A
Cuts at: 4558 4558
Size: 7222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2882 3706 2882
Size: 824 6398
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 3001 4547 1870
Size: 1131 1546 4545
Fragments arranged by size:
4545 1546 1131
XmnI GAAnn'nnTTC
Cuts at: 3386 5351 3386
Size: 1965 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2754 5030 276
Size: 53 83 186 936 1220 2276 2468
Fragments arranged by size:
2468 2276 1220 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SrfI SwaI