(Circular) (Six-base) MAPSORT of: pFN21AB6531.seq Check: 3266 from: 1 to: 5803 pFN21AB6531 5803 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3866 5250 5518 1632 Size: 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AlwNI CAG_nnn'CTG Cuts at: 2393 2503 2980 3546 5118 2393 Size: 110 477 566 1572 3078 Fragments arranged by size: 3078 1572 566 477 110 ApaI G_GGCC'C Cuts at: 2237 2786 2879 2237 Size: 549 93 5161 Fragments arranged by size: 5161 549 93 ApaLI G'TGCA_C Cuts at: 2678 3860 5016 2678 Size: 1182 1156 3465 Fragments arranged by size: 3465 1182 1156 ApoI r'AATT_y Cuts at: 1493 2576 2812 3112 3338 3490 5599 1493 Size: 1083 236 300 226 152 2109 1697 Fragments arranged by size: 2109 1697 1083 300 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2700 3128 1870 Size: 73 757 428 4545 Fragments arranged by size: 4545 757 428 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2686 2867 3124 4579 618 Size: 325 217 627 899 181 257 1455 1842 Fragments arranged by size: 1842 1455 899 627 325 257 217 181 BanII G_rGCy'C Cuts at: 1391 2237 2432 2756 2786 2861 2879 2907 3122 Size: 846 195 324 30 75 18 28 215 Cuts at: 3122 1391 Size: 4072 Fragments arranged by size: 4072 846 324 215 195 75 30 28 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2141 2762 928 Size: 971 242 621 3969 Fragments arranged by size: 3969 971 621 242 BbvCI CC'TCA_GC Cuts at: 2268 2463 2268 Size: 195 5608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 2954 3220 2954 Size: 266 5537 Bme1580I G_kGCm'C Cuts at: 1790 2237 2682 2786 2872 2879 3070 3864 5020 Size: 447 445 104 86 7 191 794 1156 Cuts at: 5020 1790 Size: 2573 Fragments arranged by size: 2573 1156 794 447 445 191 104 86 7 BmgBI CAC'GTC Cuts at: 1318 2929 1318 Size: 1611 4192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2396 3534 4492 Size: 416 53 83 399 24 979 1138 958 Cuts at: 4492 442 Size: 1753 Fragments arranged by size: 1753 1138 979 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2339 4463 1239 Size: 1088 12 2124 2579 Fragments arranged by size: 2579 2124 1088 12 Bpu10I CC'TnA_GC Cuts at: 2268 2463 2268 Size: 195 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3065 3928 4793 5091 3065 Size: 863 865 298 3777 Fragments arranged by size: 3777 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2138 3610 3992 4627 Size: 53 83 186 936 605 1472 382 635 Cuts at: 4627 275 Size: 1451 Fragments arranged by size: 1472 1451 936 635 605 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2371 4236 4634 4908 5055 5466 2371 Size: 1865 398 274 147 411 2708 Fragments arranged by size: 2708 1865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 4228 Fragments arranged by size: 4228 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2927 2963 1186 Size: 844 897 36 4026 Fragments arranged by size: 4026 897 844 36 BseYI C'CCAG_C Cuts at: 2237 2997 5006 5502 2237 Size: 760 2009 496 2538 Fragments arranged by size: 2538 2009 760 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2206 2531 1163 Size: 573 470 325 4435 Fragments arranged by size: 4435 573 470 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2494 2682 2907 3864 3949 5020 2494 Size: 188 225 957 85 1071 3277 Fragments arranged by size: 3277 1071 957 225 188 85 BsmI GAATG_Cn' Cuts at: 2656 3314 3407 2656 Size: 658 93 5052 Fragments arranged by size: 5052 658 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2432 2494 2682 2756 2786 2861 Size: 399 447 195 62 188 74 30 75 Cuts at: 2861 2872 2879 2907 3070 3122 3864 3949 5020 Size: 11 7 28 163 52 742 85 1071 Cuts at: 5020 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 447 399 195 188 163 85 75 74 62 52 30 28 11 7 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 844 Size: 2314 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2716 4298 4472 65 Size: 1183 1468 1582 174 1396 Fragments arranged by size: 1582 1468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 3001 4447 4634 1700 Size: 110 501 690 1446 187 2869 Fragments arranged by size: 2869 1446 690 501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 2393 2686 794 Size: 1599 293 3911 Fragments arranged by size: 3911 1599 293 BstBI TT'CG_AA Cuts at: 2773 4607 2773 Size: 1834 3969 BstEII G'GTnAC_C Cuts at: 1184 2200 1184 Size: 1016 4787 BstXI CCAn_nnnn'nTGG Cuts at: 2555 2638 2749 2555 Size: 83 111 5609 Fragments arranged by size: 5609 111 83 BstYI r'GATC_y Cuts at: 1972 2829 3133 3500 3885 3902 5354 5440 5452 Size: 857 304 367 385 17 1452 86 12 Cuts at: 5452 5798 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 857 385 367 346 304 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2361 2361 Size: 5803 BtgI C'CryG_G Cuts at: 513 1065 2037 2436 3046 513 Size: 552 972 399 610 3270 Fragments arranged by size: 3270 972 610 552 399 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 2354 3319 4113 4133 2354 Size: 965 794 20 4024 Fragments arranged by size: 4024 965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DraIII CAC_nnn'GTG Cuts at: 2686 3012 2686 Size: 326 5477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2551 4810 809 Size: 507 1235 2259 1802 Fragments arranged by size: 2259 1802 1235 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2658 3003 Size: 54 1425 50 275 18 243 585 345 Cuts at: 3003 3250 4139 5768 8 Size: 247 889 1629 43 Fragments arranged by size: 1629 1425 889 585 345 275 247 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2356 3733 1353 Size: 654 349 1377 3423 Fragments arranged by size: 3423 1377 654 349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2905 2905 Size: 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2339 3866 4463 5250 5518 1239 Size: 393 695 12 1527 597 787 268 1524 Fragments arranged by size: 1527 1524 787 695 597 393 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2108 2108 Size: 5803 EcoO109I rG'GnC_Cy Cuts at: 2233 2234 2782 2876 2233 Size: 1 548 94 5160 Fragments arranged by size: 5160 548 94 1 EcoRI G'AATT_C Cuts at: 2576 3112 3490 2576 Size: 536 378 4889 Fragments arranged by size: 4889 536 378 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 2814 2846 4444 Size: 32 135 32 1151 32 59 32 1598 Cuts at: 4444 4476 4518 4550 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1598 1151 135 59 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 3761 4120 4279 518 Size: 648 1581 1014 359 159 2042 Fragments arranged by size: 2042 1581 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 2267 2393 3219 3896 5044 5289 1606 Size: 614 47 126 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 677 614 245 126 47 NaeI GCC'GGC Cuts at: 2313 3003 2313 Size: 690 5113 NcoI C'CATG_G Cuts at: 513 1065 2436 513 Size: 552 1371 3880 Fragments arranged by size: 3880 1371 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NgoMIV G'CCGG_C Cuts at: 2311 3001 2311 Size: 690 5113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2305 3161 4706 1132 Size: 97 1076 856 1545 2229 Fragments arranged by size: 2229 1545 1076 856 97 PasI CC'CwG_GG Cuts at: 1348 2854 2863 2872 1348 Size: 1506 9 9 4279 Fragments arranged by size: 4279 1506 9 9 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 2980 1108 Size: 1872 3931 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2233 2782 2875 2233 Size: 549 93 5161 Fragments arranged by size: 5161 549 93 PstI C_TGCA'G Cuts at: 830 2112 2389 2448 2499 3075 3155 830 Size: 1282 277 59 51 576 80 3478 Fragments arranged by size: 3478 1282 576 277 80 59 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2220 2267 2393 2220 Size: 47 126 5630 Fragments arranged by size: 5630 126 47 SacI G_AGCT'C Cuts at: 2907 2907 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2108 2385 2412 2444 2495 3071 3151 Size: 219 1063 277 27 32 51 576 80 Cuts at: 3151 4286 4967 5158 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1063 681 576 277 219 191 80 51 32 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 2702 3130 1872 Size: 830 428 4545 Fragments arranged by size: 4545 830 428 SmlI C'TyrA_G Cuts at: 820 1017 1943 3080 3907 4808 5070 820 Size: 197 926 1137 827 901 262 1553 Fragments arranged by size: 1553 1137 926 901 827 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 2305 3161 1132 Size: 1173 856 3774 Fragments arranged by size: 3774 1173 856 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 2921 1517 Size: 1404 4399 StyI C'CwwG_G Cuts at: 513 1065 2436 3242 513 Size: 552 1371 806 3074 Fragments arranged by size: 3074 1371 806 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2855 3011 3830 3847 4000 4185 4630 Size: 1255 1275 156 819 17 153 185 445 Cuts at: 4630 325 Size: 1498 Fragments arranged by size: 1498 1275 1255 819 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 4049 5761 Size: 274 80 33 51 1443 558 1514 1712 Cuts at: 5761 96 Size: 138 Fragments arranged by size: 1712 1514 1443 558 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5763 5795 5763 Size: 32 5771 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2741 2793 2741 Size: 52 5751 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 2700 3128 1870 Size: 830 428 4545 Fragments arranged by size: 4545 830 428 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI