Restriction map for
pFN21AB7230
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MluI MroI MspA1I MspI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 981 GTAC GT^AC
AflIII 925 ACATGT A^C[GA][CT]GT
AlwNI 594 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 971 GAGCAC G[AT]GC[AT]^C
BbsI 535 GTCTTC GAAGAC(2/6)
BciVI 703 GTATCC GTATCC(6/5)
BclI 302 TGATCA T^GATCA
BfaI 991 CTAG C^TAG
BlpI 375 GCTTAGC GC^T[ACGT]AGC
BmyI 971 GAGCAC G[AGT]GC[ACT]^C
BpmI 512 CTGGAG CTGGAG(16/14)
Bpu1102I 375 GCTTAGC GC^T[ACGT]AGC
BpuAI 535 GTCTTC GAAGAC(2/6)
BsiHKAI 971 GAGCAC G[AT]GC[AT]^C
BsmAI 7 GTCTC GTCTC(1/5)
Bsp1286I 971 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 925 ACATGT A^CATGT
BspMI 646 GCAGGT ACCTGC(4/8)
CelII 375 GCTTAGC GC^T[ACGT]AGC
DsaI 251 CCATGG C^C[GA][CT]GG
EcoT14I 251 CCATGG C^C[AT][AT]GG
FbaI 302 TGATCA T^GATCA
HinfI 278 GATTC G^A[ACGT]TC
HphI 281 TCACC GGTGA(8/7)
MaeI 991 CTAG C^TAG
MaeIII 437 GTTAC ^GT[ACGT]AC
MfeI 69 CAATTG C^AATTG
MunI 69 CAATTG C^AATTG
NcoI 251 CCATGG C^CATGG
RsaI 981 GTAC GT^AC
SfcI 878 CTGTAG C^T[GA][CT]AG
SspI 171 AATATT AAT^ATT
StuI 545 AGGCCT AGG^CCT
StyI 251 CCATGG C^C[AT][AT]GG
TfiI 278 GATTC G^A[AT]TC
XcmI 632 CCACCTTGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AvaII 507 GGTCC G^G[AT]CC
629 GGACC
BalI 254 TGGCCA TGG^CCA
410 TGGCCA
BamHI 154 GGATCC G^GATCC
852 GGATCC
BbvI 216 GCAGC GCAGC(8/12)
778 GCTGC
BglII 91 AGATCT A^GATCT
112 AGATCT
BsiYI 246 CCTCTCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
896 CCTTGACATGG
BslI 246 CCTCTCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
896 CCTTGACATGG
BsmFI 628 GGGAC GGGAC(10/14)
508 GTCCC
BstF5I 243 CATCC GGATG(2/0)
386 CATCC
Cfr13I 507 GGTCC G^G[ACGT]CC
629 GGACC
EaeI 254 TGGCCA [CT]^GGCC[GA]
410 TGGCCA
Fnu4HI 216 GCAGC GC^[ACGT]GC
778 GCTGC
FokI 243 CATCC GGATG(9/13)
386 CATCC
ItaI 216 GCAGC GC^[ACGT]GC
778 GCTGC
MluNI 254 TGGCCA TGG^CCA
410 TGGCCA
MscI 254 TGGCCA TGG^CCA
410 TGGCCA
MslI 282 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
715 CATGCTCATG
NspI 714 ACATGC [GA]CATG^[CT]
925 ACATGT
Sau96I 507 GGTCC G^G[ACGT]CC
629 GGACC
SfaNI 372 GATGC GCATC(5/9)
892 GATGC
TseI 216 GCAGC G^C[AT]GC
778 GCTGC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
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AcsI AluI AlwI ApoI BsaJI
BsrI BssKI BstNI BstYI DdeI
DpnII EcoRII HaeIII MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI Tru9I
Tsp509I TspRI XhoII