(Circular) (Six-base) MAPSORT of: pFN21AB6433.seq Check: 6955 from: 1 to: 5644 pFN21AB6433 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2270 2270 Size: 5644 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 3707 5091 5359 1632 Size: 580 1495 1384 268 1917 Fragments arranged by size: 1917 1495 1384 580 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2444 2615 1166 Size: 1278 171 4195 Fragments arranged by size: 4195 1278 171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2278 1184 Size: 1094 4550 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaI G_GGCC'C Cuts at: 2421 2780 2796 2421 Size: 359 16 5269 Fragments arranged by size: 5269 359 16 ApaLI G'TGCA_C Cuts at: 2916 3701 4857 2916 Size: 785 1156 3703 Fragments arranged by size: 3703 1156 785 ApoI r'AATT_y Cuts at: 1493 2318 2953 3179 3331 5440 1493 Size: 825 635 226 152 2109 1697 Fragments arranged by size: 2109 1697 825 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2599 2723 2741 2969 1870 Size: 73 656 124 18 228 4545 Fragments arranged by size: 4545 656 228 124 73 18 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2273 2717 2737 2965 4420 Size: 325 217 627 486 444 20 228 1455 Cuts at: 4420 618 Size: 1842 Fragments arranged by size: 1842 1455 627 486 444 325 228 217 20 BanII G_rGCy'C Cuts at: 1391 2079 2157 2368 2421 2780 2796 2963 1391 Size: 688 78 211 53 359 16 167 4072 Fragments arranged by size: 4072 688 359 211 167 78 53 16 BbeI G_GCGC'C Cuts at: 2721 2721 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2101 2278 928 Size: 971 202 177 4294 Fragments arranged by size: 4294 971 202 177 BbvCI CC'TCA_GC Cuts at: 2198 2198 Size: 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2191 2225 3835 3869 1903 Size: 34 50 34 170 34 1610 34 3678 Fragments arranged by size: 3678 1610 170 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 4255 136 Size: 107 122 71 631 605 599 1984 1525 Fragments arranged by size: 1984 1525 631 605 599 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 2162 3061 2162 Size: 899 4745 Bme1580I G_kGCm'C Cuts at: 1790 2276 2421 2780 2796 2920 3705 4861 1790 Size: 486 145 359 16 124 785 1156 2573 Fragments arranged by size: 2573 1156 785 486 359 145 124 16 BmgBI CAC'GTC Cuts at: 1318 2029 1318 Size: 711 4933 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 4304 1239 Size: 902 2163 2579 Fragments arranged by size: 2579 2163 902 Bpu10I CC'TnA_GC Cuts at: 2198 2198 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2580 4307 882 Size: 617 1081 1727 2219 Fragments arranged by size: 2219 1727 1081 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2718 2748 3451 3833 Size: 53 83 186 936 1185 30 703 382 Cuts at: 3833 4468 275 Size: 635 1451 Fragments arranged by size: 1451 1185 936 703 635 382 186 83 53 30 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 1186 Size: 1110 4534 BseYI C'CCAG_C Cuts at: 2048 2685 4847 5343 2048 Size: 637 2162 496 2349 Fragments arranged by size: 2349 2162 637 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 1163 Size: 573 483 4588 Fragments arranged by size: 4588 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2646 2920 3705 3790 4861 2157 Size: 489 274 785 85 1071 2940 Fragments arranged by size: 2940 1071 785 489 274 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2157 2276 2368 2421 2646 2780 Size: 399 289 78 119 92 53 225 134 Cuts at: 2780 2796 2920 2963 3705 3790 4861 1391 Size: 16 124 43 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 225 134 124 119 92 85 78 53 43 16 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2270 2999 844 Size: 1426 729 3489 Fragments arranged by size: 3489 1426 729 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2108 4139 4313 65 Size: 1183 860 2031 174 1396 Fragments arranged by size: 2031 1396 1183 860 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2734 2849 4288 4475 1700 Size: 110 924 115 1439 187 2869 Fragments arranged by size: 2869 1439 924 187 115 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 2704 2706 1925 Size: 779 2 4863 Fragments arranged by size: 4863 779 2 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2941 2941 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639 Size: 1002 367 385 17 1452 86 12 346 Cuts at: 5639 1972 Size: 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 2877 2814 Size: 63 5581 BtgI C'CryG_G Cuts at: 513 1065 2331 2358 2439 513 Size: 552 1266 27 81 3718 Fragments arranged by size: 3718 1266 552 81 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2772 487 Size: 683 56 1546 3359 Fragments arranged by size: 3359 1546 683 56 BtsI GCAGTG_nn' Cuts at: 2869 3160 3954 3974 2869 Size: 291 794 20 4539 Fragments arranged by size: 4539 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2242 2242 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2370 2436 Size: 54 1425 50 275 18 504 36 66 Cuts at: 2436 2511 3091 3980 5609 8 Size: 75 580 889 1629 43 Fragments arranged by size: 1629 1425 889 580 504 275 75 66 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 4231 4605 4751 387 Size: 1064 138 444 2198 374 146 1280 Fragments arranged by size: 2198 1280 1064 444 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 2155 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2212 3707 4304 5091 5359 1239 Size: 393 509 71 1495 597 787 268 1524 Fragments arranged by size: 1524 1495 787 597 509 393 268 71 EcoO109I rG'GnC_Cy Cuts at: 2417 2776 2777 2793 2417 Size: 359 1 16 5268 Fragments arranged by size: 5268 359 16 1 EcoRI G'AATT_C Cuts at: 2318 2953 3331 2318 Size: 635 378 4631 Fragments arranged by size: 4631 635 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2305 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2721 4791 1678 Size: 1043 2070 2531 Fragments arranged by size: 2531 2070 1043 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 4285 4317 4359 Size: 32 135 32 719 32 1962 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1962 719 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2843 2988 3234 669 Size: 988 281 905 145 246 3079 Fragments arranged by size: 3079 988 905 281 246 145 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2717 2717 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 2438 2513 10 Size: 54 1425 343 540 66 75 3141 Fragments arranged by size: 3141 1425 540 343 75 66 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2444 2479 2615 3602 3961 4120 Size: 648 861 417 35 136 987 359 159 Cuts at: 4120 518 Size: 2042 Fragments arranged by size: 2042 987 861 648 417 359 159 136 35 MspA1I CmG'CkG Cuts at: 1606 2041 2161 2856 3060 3737 4885 5130 1606 Size: 435 120 695 204 677 1148 245 2120 Fragments arranged by size: 2120 1148 695 677 435 245 204 120 NaeI GCC'GGC Cuts at: 2851 2851 Size: 5644 NarI GG'CG_CC Cuts at: 2718 2718 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2358 513 Size: 552 1293 3799 Fragments arranged by size: 3799 1293 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2849 2849 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2136 2574 3002 4547 1132 Size: 97 907 438 428 1545 2229 Fragments arranged by size: 2229 1545 907 438 428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2198 2230 3713 3745 5226 5258 2198 Size: 32 1483 32 1481 32 2584 Fragments arranged by size: 2584 1483 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2838 1613 Size: 1225 4419 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2417 2776 2792 2417 Size: 359 16 5269 Fragments arranged by size: 5269 359 16 PstI C_TGCA'G Cuts at: 830 2391 2996 830 Size: 1561 605 3478 Fragments arranged by size: 3478 1561 605 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2041 2161 2041 Size: 120 5524 SacI G_AGCT'C Cuts at: 2157 2157 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2637 2637 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2143 2387 2992 4127 4808 4999 826 Size: 219 1098 244 605 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 681 605 244 219 191 SfoI GGC'GCC Cuts at: 2719 2719 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2743 2971 1872 Size: 871 228 4545 Fragments arranged by size: 4545 871 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2280 2358 2487 3083 513 Size: 552 1215 78 129 596 3074 Fragments arranged by size: 3074 1215 596 552 129 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 2272 2935 3671 3688 3841 4026 Size: 1255 621 71 663 736 17 153 185 Cuts at: 4026 4471 325 Size: 445 1498 Fragments arranged by size: 1498 1255 736 663 621 445 185 153 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 3890 5602 Size: 274 80 33 51 1443 267 1646 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1646 1443 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2365 2834 2011 Size: 354 469 4821 Fragments arranged by size: 4821 469 354 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2741 2969 1870 Size: 871 228 4545 Fragments arranged by size: 4545 871 228 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI