(Circular) (Six-base) MAPSORT of: pFN21AB8653.seq Check: 7037 from: 1 to: 5407 pFN21AB8653 5407 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2255 2255 Size: 5407 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2517 2728 2517 Size: 211 5196 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4854 5122 1632 Size: 1838 1384 268 1917 Fragments arranged by size: 1917 1838 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 2271 4306 5283 1203 Size: 1068 2035 977 1327 Fragments arranged by size: 2035 1327 1068 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5407 AlwNI CAG_nnn'CTG Cuts at: 3150 4722 3150 Size: 1572 3835 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2445 2732 1870 Size: 73 502 287 4545 Fragments arranged by size: 4545 502 287 73 BamHI G'GATC_C Cuts at: 2623 2737 3104 2623 Size: 114 367 4926 Fragments arranged by size: 4926 367 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2517 2528 2642 2728 Size: 325 217 627 255 475 11 114 86 Cuts at: 2728 4183 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 475 325 255 217 114 86 11 BanII G_rGCy'C Cuts at: 1391 2610 2682 2726 1391 Size: 1219 72 44 4072 Fragments arranged by size: 4072 1219 72 44 BbeI G_GCGC'C Cuts at: 2046 2532 2046 Size: 486 4921 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2348 2537 4018 Size: 107 122 71 631 605 676 189 1481 Cuts at: 4018 136 Size: 1525 Fragments arranged by size: 1525 1481 676 631 605 189 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 3468 4624 1790 Size: 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 BmgBI CAC'GTC Cuts at: 1318 2272 1318 Size: 954 4453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2625 3138 4096 Size: 416 53 83 399 24 1208 513 958 Cuts at: 4096 442 Size: 1753 Fragments arranged by size: 1753 1208 958 513 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2563 4067 1239 Size: 1324 1504 2579 Fragments arranged by size: 2579 1504 1324 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3532 4397 4695 3532 Size: 865 298 4244 Fragments arranged by size: 4244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2404 4070 882 Size: 617 905 1666 2219 Fragments arranged by size: 2219 1666 905 617 BsaAI yAC'GTr Cuts at: 493 1204 2602 3198 493 Size: 711 1398 596 2702 Fragments arranged by size: 2702 1398 711 596 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2529 3214 3596 Size: 53 83 186 936 510 486 685 382 Cuts at: 3596 4231 275 Size: 635 1451 Fragments arranged by size: 1451 936 685 635 510 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 1178 Size: 30 165 30 741 30 4411 Fragments arranged by size: 4411 741 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 1186 Size: 1000 4407 BseYI C'CCAG_C Cuts at: 2567 4610 5106 2567 Size: 2043 496 2868 Fragments arranged by size: 2868 2043 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4834 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2235 3468 3553 4624 2235 Size: 1233 85 1071 3018 Fragments arranged by size: 3018 1233 1071 85 BsiWI C'GTAC_G Cuts at: 2019 2019 Size: 5407 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2610 2682 2726 3468 3553 4624 Size: 399 445 375 72 44 742 85 1071 Cuts at: 4624 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 445 399 375 85 72 44 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2255 2386 2762 844 Size: 1411 131 376 3489 Fragments arranged by size: 3489 1411 376 131 BsrBI CCG'CTC Cuts at: 1394 1823 2027 3294 1394 Size: 429 204 1267 3507 Fragments arranged by size: 3507 1267 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2630 2656 4051 4238 1700 Size: 110 820 26 1395 187 2869 Fragments arranged by size: 2869 1395 820 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2623 2737 3104 3489 3506 4958 5044 5056 Size: 651 114 367 385 17 1452 86 12 Cuts at: 5056 5402 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 651 385 367 346 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2223 513 Size: 552 939 219 3697 Fragments arranged by size: 3697 939 552 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2440 487 Size: 683 56 1214 3454 Fragments arranged by size: 3454 1214 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2333 4414 809 Size: 507 1017 2081 1802 Fragments arranged by size: 2081 1802 1017 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2382 2658 Size: 54 1425 50 275 18 390 162 276 Cuts at: 2658 2854 3743 5372 8 Size: 196 889 1629 43 Fragments arranged by size: 1629 1425 889 390 276 275 196 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2549 3337 1353 Size: 1196 788 3423 Fragments arranged by size: 3423 1196 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 4368 4514 387 Size: 1064 138 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2563 3470 4067 4854 5122 1239 Size: 393 931 907 597 787 268 1524 Fragments arranged by size: 1524 931 907 787 597 393 268 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2046 2160 2532 4554 1678 Size: 368 114 372 2022 2531 Fragments arranged by size: 2531 2022 372 368 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2042 2528 2042 Size: 486 4921 KpnI G_GTAC'C Cuts at: 2521 2732 2521 Size: 211 5196 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 4521 4705 1305 Size: 1044 2172 184 2007 Fragments arranged by size: 2172 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 10 Size: 54 1425 343 552 3033 Fragments arranged by size: 3033 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 3365 3724 3883 518 Size: 648 951 1248 359 159 2042 Fragments arranged by size: 2042 1248 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2515 2823 3500 4648 4893 1606 Size: 909 308 677 1148 245 2120 Fragments arranged by size: 2120 1148 909 677 308 245 NaeI GCC'GGC Cuts at: 2658 2658 Size: 5407 NarI GG'CG_CC Cuts at: 2043 2529 2043 Size: 486 4921 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3916 Fragments arranged by size: 3916 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NgoMIV G'CCGG_C Cuts at: 2656 2656 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2587 2765 4310 1132 Size: 97 1358 178 1545 2229 Fragments arranged by size: 2229 1545 1358 178 97 PasI CC'CwG_GG Cuts at: 1348 2683 1348 Size: 1335 4072 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 1108 Size: 1283 4124 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 5375 PstI C_TGCA'G Cuts at: 830 2210 2759 830 Size: 1380 549 3478 Fragments arranged by size: 3478 1380 549 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2206 2755 3890 4571 4762 826 Size: 219 1161 549 1135 681 191 1471 Fragments arranged by size: 1471 1161 1135 681 549 219 191 SfoI GGC'GCC Cuts at: 2044 2530 2044 Size: 486 4921 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2447 2734 1872 Size: 575 287 4545 Fragments arranged by size: 4545 575 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3511 4412 4674 820 Size: 197 926 1568 901 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2587 2765 1132 Size: 1455 178 3774 Fragments arranged by size: 3774 1455 178 SrfI GCCC'GGGC Cuts at: 2447 2447 Size: 5407 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2004 2409 2846 513 Size: 552 939 405 437 3074 Fragments arranged by size: 3074 939 552 437 405 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 3653 5365 Size: 274 80 33 51 1443 297 1379 1712 Cuts at: 5365 96 Size: 138 Fragments arranged by size: 1712 1443 1379 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2445 2732 1870 Size: 575 287 4545 Fragments arranged by size: 4545 575 287 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI