(Circular) (Six-base) MAPSORT of: pFN21AB6334.seq Check: 3466 from: 1 to: 5572 pFN21AB6334 5572 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2309 3635 5019 5287 1632 Size: 416 261 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 416 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 2158 2643 4471 5448 1203 Size: 955 485 1828 977 1327 Fragments arranged by size: 1828 1327 977 955 485 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 3315 4887 3315 Size: 1572 4000 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2897 1870 Size: 73 954 4545 Fragments arranged by size: 4545 954 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2893 4348 618 Size: 325 217 627 1106 1455 1842 Fragments arranged by size: 1842 1455 1106 627 325 217 BanII G_rGCy'C Cuts at: 1391 2891 1391 Size: 1500 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4601 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2148 5567 2148 Size: 3419 2153 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 3633 4789 1790 Size: 1843 1156 2573 Fragments arranged by size: 2573 1843 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2519 2636 4232 1239 Size: 800 480 117 1596 2579 Fragments arranged by size: 2579 1596 800 480 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2400 3697 4562 4860 2400 Size: 1297 865 298 3112 Fragments arranged by size: 3112 1297 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2731 3363 493 Size: 711 1527 632 2702 Fragments arranged by size: 2702 1527 711 632 BsaBI GATnn'nnATC Cuts at: 2078 2801 5566 2078 Size: 723 2765 2084 Fragments arranged by size: 2765 2084 723 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 4005 4403 4677 4824 5235 2277 Size: 1728 398 274 147 411 2614 Fragments arranged by size: 2614 1728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4228 Fragments arranged by size: 4228 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2507 2516 1186 Size: 994 327 9 4242 Fragments arranged by size: 4242 994 327 9 BseYI C'CCAG_C Cuts at: 2358 2827 4775 5271 2358 Size: 469 1948 496 2659 Fragments arranged by size: 2659 1948 496 469 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 1163 Size: 573 448 4551 Fragments arranged by size: 4551 573 448 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 3783 4811 1540 Size: 275 186 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3633 3718 4789 3633 Size: 85 1071 4416 Fragments arranged by size: 4416 1071 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2891 3633 3718 4789 1391 Size: 399 1101 742 85 1071 2174 Fragments arranged by size: 2174 1101 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2277 5235 2277 Size: 2958 2614 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2927 844 Size: 2083 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4067 4241 65 Size: 1183 2819 174 1396 Fragments arranged by size: 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 2742 3632 4644 2742 Size: 890 1012 3670 Fragments arranged by size: 3670 1012 890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstXI CCAn_nnnn'nTGG Cuts at: 2668 2668 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2148 2902 3269 3654 3671 5123 5209 5221 Size: 176 754 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 754 385 367 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5020 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2641 487 Size: 683 56 1415 3418 Fragments arranged by size: 3418 1415 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2722 2722 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 3019 3908 Size: 54 1425 50 275 18 696 493 889 Cuts at: 3908 5537 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 696 493 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 3502 1353 Size: 932 1217 3423 Fragments arranged by size: 3423 1217 932 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2048 2309 2519 2636 3635 4232 Size: 393 407 9 261 210 117 999 597 Cuts at: 4232 5019 5287 1239 Size: 787 268 1524 Fragments arranged by size: 1524 999 787 597 407 393 268 261 210 117 9 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2312 2344 4213 Size: 32 135 32 563 32 145 32 1869 Cuts at: 4213 4245 4287 4319 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1869 563 145 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 4686 4870 1305 Size: 1185 2196 184 2007 Fragments arranged by size: 2196 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3750 Fragments arranged by size: 3750 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 2660 2666 3530 3889 4048 518 Size: 648 1482 12 6 864 359 159 2042 Fragments arranged by size: 2042 1482 864 648 359 159 12 6 MspA1I CmG'CkG Cuts at: 1606 2026 2041 2455 2988 3665 4813 5058 1606 Size: 420 15 414 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 533 420 414 245 15 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5020 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2162 2647 2930 4475 1132 Size: 97 933 485 283 1545 2229 Fragments arranged by size: 2229 1545 933 485 283 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5572 PciI A'CATG_T Cuts at: 2158 2643 4471 2158 Size: 485 1828 3259 Fragments arranged by size: 3259 1828 485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PmlI CAC'GTG Cuts at: 2731 2731 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2924 830 Size: 2094 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2026 2041 2026 Size: 15 5557 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SapI GCTCTTCn'nnn_ Cuts at: 2285 2285 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SfcI C'TryA_G Cuts at: 826 1045 2182 2920 4055 4736 4927 826 Size: 219 1137 738 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 738 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2415 3676 4577 4839 820 Size: 197 926 472 1261 901 262 1553 Fragments arranged by size: 1553 1261 926 901 472 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2223 3011 513 Size: 552 1158 788 3074 Fragments arranged by size: 3074 1158 788 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3599 3616 3769 3954 4399 325 Size: 1255 2019 17 153 185 445 1498 Fragments arranged by size: 2019 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI