(Circular) (Six-base) MAPSORT of: pFN21AB6374.seq Check: 3792 from: 1 to: 5533 pFN21AB6374 5533 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2268 2291 3596 4980 5248 1632 Size: 421 215 23 1305 1384 268 1917 Fragments arranged by size: 1917 1384 1305 421 268 215 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 2025 4432 5409 1203 Size: 822 2407 977 1327 Fragments arranged by size: 2407 1327 977 822 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 3276 4848 3276 Size: 1572 3961 ApaI G_GGCC'C Cuts at: 2046 2055 2046 Size: 9 5524 ApaLI G'TGCA_C Cuts at: 2687 3590 4746 2687 Size: 903 1156 3474 Fragments arranged by size: 3474 1156 903 ApoI r'AATT_y Cuts at: 1493 2137 2842 3068 3220 5329 1493 Size: 644 705 226 152 2109 1697 Fragments arranged by size: 2109 1697 705 644 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2047 2858 1870 Size: 73 95 9 811 4545 Fragments arranged by size: 4545 811 95 73 9 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 4309 618 Size: 325 217 627 1067 1455 1842 Fragments arranged by size: 1842 1455 1067 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2055 2852 1391 Size: 655 9 797 4072 Fragments arranged by size: 4072 797 655 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2771 3425 4635 2771 Size: 654 1210 3669 Fragments arranged by size: 3669 1210 654 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 4144 136 Size: 107 122 71 631 605 378 2094 1525 Fragments arranged by size: 2094 1525 631 605 378 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2950 2950 Size: 5533 Bme1580I G_kGCm'C Cuts at: 1790 2046 2055 2691 3594 4750 1790 Size: 256 9 636 903 1156 2573 Fragments arranged by size: 2573 1156 903 636 256 9 BmgBI CAC'GTC Cuts at: 1318 2088 2692 1318 Size: 770 604 4159 Fragments arranged by size: 4159 770 604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 4222 442 Size: 416 53 83 399 24 1847 958 1753 Fragments arranged by size: 1847 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2597 4193 1239 Size: 1358 1596 2579 Fragments arranged by size: 2579 1596 1358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2155 3658 4523 4821 2155 Size: 1503 865 298 2867 Fragments arranged by size: 2867 1503 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2467 4196 882 Size: 617 968 1729 2219 Fragments arranged by size: 2219 1729 968 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 2598 2759 5527 2598 Size: 161 2768 2604 Fragments arranged by size: 2768 2604 161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3340 3722 4357 275 Size: 53 83 186 936 1807 382 635 1451 Fragments arranged by size: 1807 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 3966 4364 4638 4785 5196 2031 Size: 1935 398 274 147 411 2368 Fragments arranged by size: 2368 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2495 1186 Size: 1279 30 4224 Fragments arranged by size: 4224 1279 30 BseYI C'CCAG_C Cuts at: 2055 4736 5232 2055 Size: 2681 496 2356 Fragments arranged by size: 2681 2356 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2110 2384 1163 Size: 573 374 274 4312 Fragments arranged by size: 4312 573 374 274 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 3594 3679 4750 2691 Size: 903 85 1071 3474 Fragments arranged by size: 3474 1071 903 85 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2055 2691 2852 3594 3679 4750 Size: 399 256 9 636 161 742 85 1071 Cuts at: 4750 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 636 399 256 161 85 9 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 2322 3422 5152 2322 Size: 1100 1730 2703 Fragments arranged by size: 2703 1730 1100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2888 844 Size: 2044 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2417 2863 3230 3615 3632 5084 5170 5182 Size: 445 446 367 385 17 1452 86 12 Cuts at: 5182 5528 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 446 445 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4981 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 2121 3049 3843 3863 2121 Size: 928 794 20 3791 Fragments arranged by size: 3791 928 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2611 2837 2611 Size: 226 5307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 2619 2980 Size: 54 1425 50 275 18 687 102 361 Cuts at: 2980 3869 5498 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 687 361 275 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2430 3463 1353 Size: 1077 1033 3423 Fragments arranged by size: 3423 1077 1033 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4120 4494 4640 387 Size: 1064 138 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2268 2291 2597 3596 4193 4980 Size: 393 421 215 23 306 999 597 787 Cuts at: 4980 5248 1239 Size: 268 1524 Fragments arranged by size: 1524 999 787 597 421 393 306 268 215 23 EcoO109I rG'GnC_Cy Cuts at: 2042 2043 2051 2052 2259 2042 Size: 1 8 1 207 5316 Fragments arranged by size: 5316 207 8 1 1 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2296 4680 1678 Size: 618 2384 2531 Fragments arranged by size: 2531 2384 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2793 2825 4174 4206 4248 Size: 32 135 32 1221 32 1349 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 1349 1221 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 2621 10 Size: 54 1425 343 687 102 2922 Fragments arranged by size: 2922 1425 687 343 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3850 4009 518 Size: 648 2325 359 159 2042 Fragments arranged by size: 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2107 2458 2575 2949 3626 4774 5019 Size: 488 13 351 117 374 677 1148 245 Cuts at: 5019 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 488 374 351 245 117 13 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4981 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2029 2891 4436 1132 Size: 97 800 862 1545 2229 Fragments arranged by size: 2229 1545 862 800 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 2025 4432 2025 Size: 2407 3126 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2061 2061 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2259 2259 Size: 5533 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 2823 3103 2823 Size: 280 5253 PspOMI G'GGCC_C Cuts at: 2042 2051 2042 Size: 9 5524 PstI C_TGCA'G Cuts at: 830 2341 2434 2885 830 Size: 1511 93 451 3478 Fragments arranged by size: 3478 1511 451 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2458 2575 2458 Size: 117 5416 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2180 2337 2430 2881 4016 4697 4888 Size: 219 1135 157 93 451 1135 681 191 Cuts at: 4888 826 Size: 1471 Fragments arranged by size: 1471 1135 1135 681 451 219 191 157 93 SfiI GGCCn_nnn'nGGCC Cuts at: 2050 2050 Size: 5533 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2040 2860 1872 Size: 168 820 4545 Fragments arranged by size: 4545 820 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 2170 3637 4538 4800 820 Size: 197 926 227 1467 901 262 1553 Fragments arranged by size: 1553 1467 926 901 262 227 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2226 2972 513 Size: 552 1161 746 3074 Fragments arranged by size: 3074 1161 746 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3560 3577 3730 3915 4360 325 Size: 1255 1980 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2038 2858 1870 Size: 168 820 4545 Fragments arranged by size: 4545 820 168 XmnI GAAnn'nnTTC Cuts at: 2330 3662 2330 Size: 1332 4201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI