(Circular) (Six-base) MAPSORT of: pFN21AB7244.seq Check: 8794 from: 1 to: 5797 pFN21AB7244 5797 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2265 3607 278 Size: 53 83 186 936 729 1342 2468 Fragments arranged by size: 2468 1342 936 729 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 2555 3118 2555 Size: 563 5234 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 5244 5512 1632 Size: 2228 1384 268 1917 Fragments arranged by size: 2228 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2621 1166 Size: 1455 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2534 1184 Size: 1350 4447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2033 2065 2294 2326 2000 Size: 32 1 32 229 32 5471 Fragments arranged by size: 5471 229 32 32 32 1 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2119 2119 Size: 5797 ApaLI G'TGCA_C Cuts at: 2429 3854 5010 2429 Size: 1425 1156 3216 Fragments arranged by size: 3216 1425 1156 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2118 3122 1870 Size: 73 110 65 1004 4545 Fragments arranged by size: 4545 1004 110 73 65 BamHI G'GATC_C Cuts at: 3021 3127 3494 3021 Size: 106 367 5324 Fragments arranged by size: 5324 367 106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2131 2454 2555 2597 Size: 325 217 627 314 30 323 101 42 Cuts at: 2597 2981 3118 4573 618 Size: 384 137 1455 1842 Fragments arranged by size: 1842 1455 627 384 325 323 314 217 137 101 42 30 BanII G_rGCy'C Cuts at: 1391 2013 2119 2444 2583 2844 2978 3116 1391 Size: 622 106 325 139 261 134 138 4072 Fragments arranged by size: 4072 622 325 261 139 138 134 106 BbeI G_GCGC'C Cuts at: 2105 2458 2601 2105 Size: 353 143 5301 Fragments arranged by size: 5301 353 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 2377 2596 928 Size: 971 326 152 219 4129 Fragments arranged by size: 4129 971 326 219 152 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2477 2587 4408 Size: 107 122 71 631 605 805 110 1821 Cuts at: 4408 136 Size: 1525 Fragments arranged by size: 1821 1525 805 631 605 122 110 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2438 3214 2438 Size: 776 5021 Bme1580I G_kGCm'C Cuts at: 1790 2119 2136 2433 3858 5014 1790 Size: 329 17 297 1425 1156 2573 Fragments arranged by size: 2573 1425 1156 329 297 17 BmgBI CAC'GTC Cuts at: 1318 2542 1318 Size: 1224 4573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 4457 1239 Size: 849 2369 2579 Fragments arranged by size: 2579 2369 849 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2194 3588 493 Size: 711 990 1394 2702 Fragments arranged by size: 2702 1394 990 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 2262 2364 2455 Size: 53 83 186 936 569 160 102 91 Cuts at: 2455 2598 2997 3604 3986 4621 275 Size: 143 399 607 382 635 1451 Fragments arranged by size: 1451 936 635 607 569 399 382 186 160 143 102 91 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2033 2063 1178 Size: 30 165 30 597 30 3 30 4912 Fragments arranged by size: 4912 597 165 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 1186 Size: 852 4945 BseYI C'CCAG_C Cuts at: 2020 2033 2167 2275 2626 5000 5496 2020 Size: 13 134 108 351 2374 496 2321 Fragments arranged by size: 2374 2321 496 351 134 108 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 1163 Size: 573 828 4396 Fragments arranged by size: 4396 828 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2163 2622 3247 4008 5036 1540 Size: 275 186 162 459 625 761 1028 2301 Fragments arranged by size: 2301 1028 761 625 459 275 186 162 BsiHKAI G_wGCw'C Cuts at: 2433 2444 3858 3943 5014 2433 Size: 11 1414 85 1071 3216 Fragments arranged by size: 3216 1414 1071 85 11 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2119 2136 2433 2444 2583 2844 Size: 399 223 106 17 297 11 139 261 Cuts at: 2844 2978 3116 3858 3943 5014 1391 Size: 134 138 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 261 223 139 138 134 106 85 17 11 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 2349 2400 3686 5416 2349 Size: 51 1286 1730 2730 Fragments arranged by size: 2730 1730 1286 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2567 2676 3152 844 Size: 1310 413 109 476 3489 Fragments arranged by size: 3489 1310 476 413 109 BsrBI CCG'CTC Cuts at: 1394 1823 2524 2734 3684 1394 Size: 429 701 210 950 3507 Fragments arranged by size: 3507 950 701 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2852 4441 4628 1700 Size: 110 288 754 1589 187 2869 Fragments arranged by size: 2869 1589 754 288 187 110 BsrGI T'GTAC_A Cuts at: 96 2268 96 Size: 2172 3625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 3094 3094 Size: 5797 BstYI r'GATC_y Cuts at: 1972 3021 3127 3494 3879 3896 5348 5434 5446 Size: 1049 106 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1049 385 367 346 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 2583 2823 3000 513 Size: 552 939 483 96 240 177 3310 Fragments arranged by size: 3310 939 552 483 240 177 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2259 2430 3313 4107 4127 2259 Size: 171 883 794 20 3929 Fragments arranged by size: 3929 883 794 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2256 2292 Size: 54 1425 50 275 18 330 96 36 Cuts at: 2292 2469 2573 2619 2820 3244 4133 5762 8 Size: 177 104 46 201 424 889 1629 43 Fragments arranged by size: 1629 1425 889 424 330 275 201 177 104 96 54 50 46 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2160 2619 3244 1537 Size: 275 348 459 625 4090 Fragments arranged by size: 4090 625 459 348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2839 2927 4384 4758 4904 387 Size: 1064 138 1250 88 1457 374 146 1280 Fragments arranged by size: 1457 1280 1250 1064 374 146 138 88 EcoICRI GAG'CTC Cuts at: 2442 2442 Size: 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 3860 4457 5244 5512 1239 Size: 393 456 1772 597 787 268 1524 Fragments arranged by size: 1772 1524 787 597 456 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2969 2969 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2115 2497 2115 Size: 382 5415 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2105 2458 2487 2601 2728 2831 4944 1678 Size: 427 353 29 114 127 103 2113 2531 Fragments arranged by size: 2531 2113 427 353 127 114 103 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373 Size: 32 135 32 2866 32 42 32 2626 Fragments arranged by size: 2866 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2368 2806 2815 3141 3387 669 Size: 988 281 430 438 9 326 246 3079 Fragments arranged by size: 3079 988 438 430 326 281 246 9 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2101 2454 2597 2101 Size: 353 143 5301 Fragments arranged by size: 5301 353 143 KpnI G_GTAC'C Cuts at: 2559 3122 2559 Size: 563 5234 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 2471 10 Size: 54 1425 343 462 177 3336 Fragments arranged by size: 3336 1425 462 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2405 2621 2678 3755 4114 4273 Size: 648 1026 213 216 57 1077 359 159 Cuts at: 4273 518 Size: 2042 Fragments arranged by size: 2042 1077 1026 648 359 216 213 159 57 MspA1I CmG'CkG Cuts at: 1606 2037 2437 2451 2489 2571 2644 2895 3213 Size: 431 400 14 38 82 73 251 318 Cuts at: 3213 3890 5038 5283 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 431 400 318 251 245 82 73 38 14 NaeI GCC'GGC Cuts at: 2100 2854 2100 Size: 754 5043 NarI GG'CG_CC Cuts at: 2102 2455 2598 2102 Size: 353 143 5301 Fragments arranged by size: 5301 353 143 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4306 Fragments arranged by size: 4306 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NgoMIV G'CCGG_C Cuts at: 2098 2852 2098 Size: 754 5043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2275 2515 3155 4700 1132 Size: 97 1046 240 640 1545 2229 Fragments arranged by size: 2229 1545 1046 640 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2000 2032 2033 2065 3866 3898 5379 5411 2000 Size: 32 1 32 1801 32 1481 32 2386 Fragments arranged by size: 2386 1801 1481 32 32 32 32 1 PpuMI rG'GwC_Cy Cuts at: 2497 2497 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2115 2115 Size: 5797 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2037 2571 2895 2037 Size: 534 324 4939 Fragments arranged by size: 4939 534 324 SacI G_AGCT'C Cuts at: 2444 2444 Size: 5797 SacII CC_GC'GG Cuts at: 2490 2490 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 3145 4280 4961 5152 826 Size: 219 2100 1135 681 191 1471 Fragments arranged by size: 2100 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2103 2456 2599 2103 Size: 353 143 5301 Fragments arranged by size: 5301 353 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 2055 2120 3124 1872 Size: 183 65 1004 4545 Fragments arranged by size: 4545 1004 183 65 SmlI C'TyrA_G Cuts at: 820 1017 1943 3901 4802 5064 820 Size: 197 926 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SrfI GCCC'GGGC Cuts at: 2120 2120 Size: 5797 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2004 2985 3236 513 Size: 552 939 981 251 3074 Fragments arranged by size: 3074 981 939 552 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 4043 5755 Size: 274 80 33 51 1443 291 1775 1712 Cuts at: 5755 96 Size: 138 Fragments arranged by size: 1775 1712 1443 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 2363 1316 Size: 1047 4750 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 2053 2118 3122 1870 Size: 183 65 1004 4545 Fragments arranged by size: 4545 1004 183 65 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2263 3605 276 Size: 53 83 186 936 729 1342 2468 Fragments arranged by size: 2468 1342 936 729 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SwaI XcmI