Restriction map for
pFN21AB6326
##################################
# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 405 GAATTT [GA]^AATT[CT]
AlwI 847 GGATC GGATC(4/5)
ApoI 405 GAATTT [GA]^AATT[CT]
Asp700I 277 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 484 GAGCCC G[GA]GC[CT]^C
BbsI 565 GTCTTC GAAGAC(2/6)
BglI 71 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 99 AGATCT A^GATCT
BpuAI 565 GTCTTC GAAGAC(2/6)
BsaI 705 GAGACC GGTCTC(1/5)
BseRI 7 CTCCTC GAGGAG(10/8)
BsgI 245 GTGCAG GTGCAG(16/14)
BsrDI 545 GCAATG GCAATG(2/0)
BsrI 390 CCAGT ACTGG(1/-1)
BssSI 37 CTCGTG CACGAG(-5/-1)
BstXI 182 CCACAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 17 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 70 GGCCC G^G[ACGT]CC
DraIII 239 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 488 CCATGG C^C[GA][CT]GG
EcoT14I 488 CCATGG C^C[AT][AT]GG
FauI 14 CCCGC CCCGC(4/6)
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
MaeIII 580 GTTAC ^GT[ACGT]AC
MslI 429 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 71 GCCCCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 488 CCATGG C^CATGG
NlaIV 70 GGCCCC GG[ACGT]^[ACGT]CC
PleI 5 GACTC GAGTC(4/5)
PstI 479 CTGCAG CTGCA^G
SapI 415 GCTCTTC GCTCTTC(1/4)
Sau96I 70 GGCCC G^G[ACGT]CC
ScaI 464 AGTACT AGT^ACT
StyI 488 CCATGG C^C[AT][AT]GG
XbaI 691 TCTAGA T^CTAGA
XmnI 277 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 379 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
BbvI 21 GCTGC GCAGC(8/12)
24 GCTGC
BfaI 685 CTAG C^TAG
692 CTAG
BmyI 484 GAGCCC G[AGT]GC[ACT]^C
827 GGGCAC
Bsp1286I 484 GAGCCC G[AGT]GC[ACT]^C
827 GGGCAC
BssKI 309 CCTGG ^CC[ACGT]GG
818 CCAGG
BstNI 309 CCTGG CC^[AT]GG
818 CCAGG
BstYI 99 AGATCT [GA]^GATC[CT]
847 GGATCT
Bsu36I 379 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
EarI 416 CTCTTC CTCTTC(1/4)
834 GAAGAG
Eco81I 379 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
EcoRII 309 CCTGG ^CC[AT]GG
818 CCAGG
HaeIII 70 GGCC GG^CC
670 GGCC
HindIII 233 AAGCTT A^AGCTT
797 AAGCTT
Ksp632I 416 CTCTTC CTCTTC(1/4)
834 GAAGAG
MaeI 685 CTAG C^TAG
692 CTAG
MflI 99 AGATCT [GA]^GATC[CT]
847 GGATCT
MvaI 309 CCTGG CC^[AT]GG
818 CCAGG
NlaIII 489 CATG CATG^
756 CATG
ScrFI 309 CCTGG CC^[ACGT]GG
818 CCAGG
SfaNI 361 GATGC GCATC(5/9)
559 GATGC
SfcI 431 CTGTAG C^T[GA][CT]AG
479 CTGCAG
TseI 21 GCTGC G^C[AT]GC
24 GCTGC
Tsp509I 138 AATT ^AATT
406 AATT
XhoII 99 AGATCT [GA]^GATC[CT]
847 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI AluI BsaJI BsiYI
BslI BsmAI BstF5I DdeI DpnII
Fnu4HI FokI HinfI HphI ItaI
MboI MboII MnlI NdeII RsaI
Sau3AI TfiI TspRI