Restriction map for
pFN21AB7778
(Linear) (Six-base) MAPSORT of: KIBB7778.seq Check: 9334 from: 1 to: 1524
KIBB7778.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1314 1524
Size: 1314 210
AatII G_ACGT'C
Cuts at: 0 1189 1524
Size: 1189 335
AccI GT'mk_AC
Cuts at: 0 583 1524
Size: 583 941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 465 808 988 1524
Size: 313 152 343 180 536
Fragments arranged by size:
536 343 313 180 152
AhdI GACnn_n'nnGTC
Cuts at: 0 249 1524
Size: 249 1275
AleI CACnn'nnGTG
Cuts at: 0 1204 1524
Size: 1204 320
AlwNI CAG_nnn'CTG
Cuts at: 0 549 919 1524
Size: 549 370 605
Fragments arranged by size:
605 549 370
ApaI G_GGCC'C
Cuts at: 0 158 222 341 1524
Size: 158 64 119 1183
Fragments arranged by size:
1183 158 119 64
ApaLI G'TGCA_C
Cuts at: 0 1084 1524
Size: 1084 440
AvaI C'yCGr_G
Cuts at: 0 133 151 340 419 1260 1524
Size: 133 18 189 79 841 264
Fragments arranged by size:
841 264 189 133 79 18
BanI G'GyrC_C
Cuts at: 0 204 274 909 1308 1524
Size: 204 70 635 399 216
Fragments arranged by size:
635 399 216 204 70
BanII G_rGCy'C
Cuts at: 0 46 152 158 222 341 1524
Size: 46 106 6 64 119 1183
Fragments arranged by size:
1183 119 106 64 46 6
BbeI G_GCGC'C
Cuts at: 0 208 278 1524
Size: 208 70 1246
Fragments arranged by size:
1246 208 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 499 1524
Size: 421 78 1025
Fragments arranged by size:
1025 421 78
BclI T'GATC_A
Cuts at: 0 614 1524
Size: 614 910
BglI GCCn_nnn'nGGC
Cuts at: 0 28 1524
Size: 28 1496
BglII A'GATC_T
Cuts at: 0 732 1524
Size: 732 792
Bme1580I G_kGCm'C
Cuts at: 0 158 222 341 1088 1199 1524
Size: 158 64 119 747 111 325
Fragments arranged by size:
747 325 158 119 111 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 929 1060 1524
Size: 929 131 464
Fragments arranged by size:
929 464 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 1524
Size: 261 1263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1134 1524
Size: 187 947 390
Fragments arranged by size:
947 390 187
Bpu10I CC'TnA_GC
Cuts at: 0 304 1524
Size: 304 1220
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 766 1524
Size: 619 147 758
Fragments arranged by size:
758 619 147
BsaBI GATnn'nnATC
Cuts at: 0 316 1524
Size: 316 1208
BsaHI Gr'CG_yC
Cuts at: 0 205 275 346 1186 1358 1524
Size: 205 70 71 840 172 166
Fragments arranged by size:
840 205 172 166 71 70
BsaWI w'CCGG_w
Cuts at: 0 68 1524
Size: 68 1456
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 277 280 984 1137 1524
Size: 274 3 3 704 153 387
Fragments arranged by size:
704 387 274 153 3 3
BseYI C'CCAG_C
Cuts at: 0 16 270 1055 1524
Size: 16 254 785 469
Fragments arranged by size:
785 469 254 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1417 1524
Size: 1090 327 107
Fragments arranged by size:
1090 327 107
BsiEI CG_ry'CG
Cuts at: 0 88 162 1524
Size: 88 74 1362
Fragments arranged by size:
1362 88 74
BsiHKAI G_wGCw'C
Cuts at: 0 1088 1524
Size: 1088 436
BsmI GAATG_Cn'
Cuts at: 0 1299 1524
Size: 1299 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1112 1524
Size: 1112 412
Bsp1286I G_dGCh'C
Cuts at: 0 46 152 158 222 341 1088 1199 1524
Size: 46 106 6 64 119 747 111 325
Fragments arranged by size:
747 325 119 111 106 64 46 6
BspHI T'CATG_A
Cuts at: 0 503 1181 1524
Size: 503 678 343
Fragments arranged by size:
678 503 343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 658 821 1050 1314 1524
Size: 658 163 229 264 210
Fragments arranged by size:
658 264 229 210 163
BsrBI CCG'CTC
Cuts at: 0 171 282 383 395 1524
Size: 171 111 101 12 1129
Fragments arranged by size:
1129 171 111 101 12
BsrFI r'CCGG_y
Cuts at: 0 375 628 792 1524
Size: 375 253 164 732
Fragments arranged by size:
732 375 253 164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 906 1206 1524
Size: 906 300 318
Fragments arranged by size:
906 318 300
BstYI r'GATC_y
Cuts at: 0 732 1400 1524
Size: 732 668 124
Fragments arranged by size:
732 668 124
BstZ17I GTA'TAC
Cuts at: 0 584 1524
Size: 584 940
BtgI C'CryG_G
Cuts at: 0 23 212 1524
Size: 23 189 1312
Fragments arranged by size:
1312 189 23
BtsI GCAGTG_nn'
Cuts at: 0 771 954 1055 1524
Size: 771 183 101 469
Fragments arranged by size:
771 469 183 101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 702 737 1524
Size: 702 35 787
Fragments arranged by size:
787 702 35
EaeI y'GGCC_r
Cuts at: 0 85 159 356 560 1212 1524
Size: 85 74 197 204 652 312
Fragments arranged by size:
652 312 204 197 85 74
EagI C'GGCC_G
Cuts at: 0 85 159 1524
Size: 85 74 1365
Fragments arranged by size:
1365 85 74
EarI CTCTTCn'nnn_
Cuts at: 0 719 964 1524
Size: 719 245 560
Fragments arranged by size:
719 560 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 313 465 808 988 1134 1524
Size: 187 126 152 343 180 146 390
Fragments arranged by size:
390 343 187 180 152 146 126
EcoO109I rG'GnC_Cy
Cuts at: 0 129 218 219 1002 1062 1092 1524
Size: 129 89 1 783 60 30 432
Fragments arranged by size:
783 432 129 89 60 30 1
HaeII r_GCGC'y
Cuts at: 0 208 278 471 1524
Size: 208 70 193 1053
Fragments arranged by size:
1053 208 193 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 1524
Size: 235 32 1257
Fragments arranged by size:
1257 235 32
KasI G'GCGC_C
Cuts at: 0 204 274 1524
Size: 204 70 1250
Fragments arranged by size:
1250 204 70
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 1524
Size: 731 793
MscI TGG'CCA
Cuts at: 0 562 1214 1524
Size: 562 652 310
Fragments arranged by size:
652 562 310
MslI CAynn'nnrTG
Cuts at: 0 1033 1081 1180 1204 1324 1524
Size: 1033 48 99 24 120 200
Fragments arranged by size:
1033 200 120 99 48 24
MspA1I CmG'CkG
Cuts at: 0 25 187 214 230 355 878 983 1014
Size: 25 162 27 16 125 523 105 31
Cuts at: 1014 1154 1524
Size: 140 370
Fragments arranged by size:
523 370 162 140 125 105 31 27
25 16
NaeI GCC'GGC
Cuts at: 0 794 1524
Size: 794 730
NarI GG'CG_CC
Cuts at: 0 205 275 1524
Size: 205 70 1249
Fragments arranged by size:
1249 205 70
NgoMIV G'CCGG_C
Cuts at: 0 792 1524
Size: 792 732
NspI r_CATG'y
Cuts at: 0 1240 1524
Size: 1240 284
PasI CC'CwG_GG
Cuts at: 0 51 1524
Size: 51 1473
PflMI CCAn_nnn'nTGG
Cuts at: 0 1431 1524
Size: 1431 93
PfoI T'CCnGG_A
Cuts at: 0 1343 1524
Size: 1343 181
PpuMI rG'GwC_Cy
Cuts at: 0 129 1062 1524
Size: 129 933 462
Fragments arranged by size:
933 462 129
PspOMI G'GGCC_C
Cuts at: 0 154 218 337 1524
Size: 154 64 119 1187
Fragments arranged by size:
1187 154 119 64
PstI C_TGCA'G
Cuts at: 0 608 1524
Size: 608 916
PvuII CAG'CTG
Cuts at: 0 1014 1524
Size: 1014 510
SacII CC_GC'GG
Cuts at: 0 26 215 1524
Size: 26 189 1309
Fragments arranged by size:
1309 189 26
SanDI GG'GwC_CC
Cuts at: 0 129 1524
Size: 129 1395
SexAI A'CCwGG_T
Cuts at: 0 743 1524
Size: 743 781
SfcI C'TryA_G
Cuts at: 0 225 604 1480 1524
Size: 225 379 876 44
Fragments arranged by size:
876 379 225 44
SfoI GGC'GCC
Cuts at: 0 206 276 1524
Size: 206 70 1248
Fragments arranged by size:
1248 206 70
SmaI CCC'GGG
Cuts at: 0 135 421 1524
Size: 135 286 1103
Fragments arranged by size:
1103 286 135
SphI G_CATG'C
Cuts at: 0 1240 1524
Size: 1240 284
StuI AGG'CCT
Cuts at: 0 514 1524
Size: 514 1010
StyI C'CwwG_G
Cuts at: 0 776 1524
Size: 776 748
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1198 1258 1524
Size: 1198 60 266
Fragments arranged by size:
1198 266 60
TatI w'GTAC_w
Cuts at: 0 973 1524
Size: 973 551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1263 1351 1524
Size: 1263 88 173
Fragments arranged by size:
1263 173 88
XmaI C'CCGG_G
Cuts at: 0 133 419 1524
Size: 133 286 1105
Fragments arranged by size:
1105 286 133
XmnI GAAnn'nnTTC
Cuts at: 0 1297 1524
Size: 1297 227
ZraI GAC'GTC
Cuts at: 0 1187 1524
Size: 1187 337
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BclI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstXI BstYI
BstZ17I BtgI BtsI CspCI EaeI EagI EarI Eco57MI
EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacII SanDI SexAI SfcI SfoI
SmaI SphI StuI StyI TaqII TatI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaAI
BsaXI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII Bsu36I BtgZI ClaI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SapI SbfI ScaI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI