(Circular) (Six-base) MAPSORT of: pFN21AB5977.seq Check: 6857 from: 1 to: 6262 pFN21AB5977 6262 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3605 1937 Size: 1668 4594 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3315 3564 4325 5709 5977 1632 Size: 1683 249 761 1384 268 1917 Fragments arranged by size: 1917 1683 1384 761 268 249 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2602 5161 6138 1203 Size: 1399 2559 977 1327 Fragments arranged by size: 2559 1399 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AlwNI CAG_nnn'CTG Cuts at: 2830 4005 5577 2830 Size: 1175 1572 3515 Fragments arranged by size: 3515 1572 1175 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2534 3571 3797 3949 6058 1493 Size: 1041 1037 226 152 2109 1697 Fragments arranged by size: 2109 1697 1041 1037 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 3587 1870 Size: 73 1644 4545 Fragments arranged by size: 4545 1644 73 BamHI G'GATC_C Cuts at: 2767 3592 3959 2767 Size: 825 367 5070 Fragments arranged by size: 5070 825 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3583 5038 618 Size: 325 217 627 1796 1455 1842 Fragments arranged by size: 1842 1796 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3581 1391 Size: 2190 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 2682 928 Size: 971 236 547 4508 Fragments arranged by size: 4508 971 547 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3222 3256 4453 4487 1903 Size: 34 1285 34 1197 34 3678 Fragments arranged by size: 3678 1285 1197 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BfrBI ATG'CAT Cuts at: 2915 2915 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2483 4873 Size: 107 122 71 631 605 337 474 2390 Cuts at: 4873 136 Size: 1525 Fragments arranged by size: 2390 1525 631 605 474 337 122 107 71 BglII A'GATC_T Cuts at: 2218 6257 2218 Size: 4039 2223 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 4323 5479 1790 Size: 2533 1156 2573 Fragments arranged by size: 2573 2533 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3535 3993 4951 Size: 416 53 83 399 24 2118 458 958 Cuts at: 4951 442 Size: 1753 Fragments arranged by size: 2118 1753 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2040 2733 2040 Size: 693 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2112 2613 4922 1239 Size: 848 25 501 2309 2579 Fragments arranged by size: 2579 2309 848 501 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4387 5252 5550 4387 Size: 865 298 5099 Fragments arranged by size: 5099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4925 882 Size: 617 3426 2219 Fragments arranged by size: 3426 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4069 4451 5086 275 Size: 53 83 186 936 2536 382 635 1451 Fragments arranged by size: 2536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4695 5093 5367 5514 5925 4695 Size: 398 274 147 411 5032 Fragments arranged by size: 5032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2016 3131 1186 Size: 830 1115 4317 Fragments arranged by size: 4317 1115 830 BseYI C'CCAG_C Cuts at: 2032 5465 5961 2032 Size: 3433 496 2333 Fragments arranged by size: 3433 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5689 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3712 4473 5501 1540 Size: 275 186 12 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 4323 4408 5479 4323 Size: 85 1071 5106 Fragments arranged by size: 5106 1071 85 BsmI GAATG_Cn' Cuts at: 2479 3533 3773 3866 2479 Size: 1054 240 93 4875 Fragments arranged by size: 4875 1054 240 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3581 4323 4408 5479 1391 Size: 399 1791 742 85 1071 2174 Fragments arranged by size: 2174 1791 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2328 3555 4757 4931 65 Size: 1183 1080 1227 1202 174 1396 Fragments arranged by size: 1396 1227 1202 1183 1080 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5093 1700 Size: 110 3096 187 2869 Fragments arranged by size: 3096 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 3418 5066 3418 Size: 1648 4614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstYI r'GATC_y Cuts at: 1972 2062 2218 2381 2446 2767 3592 3959 4344 Size: 90 156 163 65 321 825 367 385 Cuts at: 4344 4361 5813 5899 5911 6257 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 825 385 367 346 321 163 156 90 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 3216 3778 4572 4592 3216 Size: 562 794 20 4886 Fragments arranged by size: 4886 794 562 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2038 2073 2176 2211 2677 2712 529 Size: 35 1474 35 103 35 466 35 4079 Fragments arranged by size: 4079 1474 466 103 35 35 35 35 DraI TTT'AAA Cuts at: 3463 3566 3463 Size: 103 6159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3709 4598 Size: 54 1425 50 275 18 180 1699 889 Cuts at: 4598 6227 8 Size: 1629 43 Fragments arranged by size: 1699 1629 1425 889 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3709 1537 Size: 275 198 1699 4090 Fragments arranged by size: 4090 1699 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 4192 1353 Size: 1032 1807 3423 Fragments arranged by size: 3423 1807 1032 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2112 2613 3315 3564 4325 4922 Size: 393 455 25 501 702 249 761 597 Cuts at: 4922 5709 5977 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 761 702 597 501 455 393 268 249 25 EcoO109I rG'GnC_Cy Cuts at: 2080 2080 Size: 6262 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 EcoRV GAT'ATC Cuts at: 3405 3405 Size: 6262 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 5409 1678 Size: 3731 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 4903 4935 4977 Size: 32 135 32 1097 32 2202 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 2202 1097 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2881 3606 3852 669 Size: 988 281 943 725 246 3079 Fragments arranged by size: 3079 988 943 725 281 246 HindIII A'AGCT_T Cuts at: 748 3147 748 Size: 2399 3863 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2777 5376 5560 1305 Size: 1472 2599 184 2007 Fragments arranged by size: 2599 2007 1472 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2979 4220 4579 4738 518 Size: 648 1813 1241 359 159 2042 Fragments arranged by size: 2042 1813 1241 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 3029 3514 3678 4355 5503 5748 1606 Size: 426 997 485 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 997 677 485 426 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NsiI A_TGCA'T Cuts at: 2917 2917 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2606 3620 5165 1132 Size: 97 1377 1014 1545 2229 Fragments arranged by size: 2229 1545 1377 1014 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 2602 5161 2602 Size: 2559 3703 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2101 4331 4363 5844 5876 2069 Size: 32 2230 32 1481 32 2455 Fragments arranged by size: 2455 2230 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2080 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 2657 1613 Size: 1044 5218 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PstI C_TGCA'G Cuts at: 830 2152 2302 3211 3614 830 Size: 1322 150 909 403 3478 Fragments arranged by size: 3478 1322 909 403 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 3029 3514 3029 Size: 485 5777 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 3482 4510 6222 3482 Size: 1028 1712 3522 Fragments arranged by size: 3522 1712 1028 SfcI C'TryA_G Cuts at: 826 1045 2148 2298 2762 3207 3610 4745 5426 Size: 219 1103 150 464 445 403 1135 681 Cuts at: 5426 5617 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1103 681 464 445 403 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4366 5267 5529 820 Size: 197 926 2423 901 262 1553 Fragments arranged by size: 2423 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 3493 152 Size: 3341 2921 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 2597 2847 4186 5 Size: 47 2545 250 1339 2081 Fragments arranged by size: 2545 2081 1339 250 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 2991 3701 513 Size: 552 939 651 336 710 3074 Fragments arranged by size: 3074 939 710 651 552 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4289 4306 4459 4644 5089 325 Size: 1255 2709 17 153 185 445 1498 Fragments arranged by size: 2709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3480 4508 6220 Size: 274 80 33 51 1443 1503 1028 1712 Cuts at: 6220 96 Size: 138 Fragments arranged by size: 1712 1503 1443 1028 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6222 6254 6222 Size: 32 6230 Tth111I GACn'n_nGTC Cuts at: 1316 2876 1316 Size: 1560 4702 XbaI T'CTAG_A Cuts at: 3086 3598 3086 Size: 512 5750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2662 2662 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI